---
title: Does adding serum and other cytokines at a high concentration to the cell suspension before mixing with VitroGel® lead to bubble formation?
description: No, there is no issue with bubble formation due to the low viscosity of VitroGel®. There is an option to warm the medium and hydrogel solution before mixing them, as this can help reduce the viscosity.
url: "https://www.thewellbio.com/docs/does-adding-serum-and-other-cytokines-at-a-high-concentration-to-the-cell-suspension-before-mixing-with-vitrogel-lead-to-bubble-formation/"
updated: "2024-08-22"
category: Injectable Hydrogel / In Vivo
tags:
  - Delivery System
  - Injection
  - Supplement
  - Xenograft
---

# Does adding serum and other cytokines at a high concentration to the cell suspension before mixing with VitroGel® lead to bubble formation?

No, there is no issue with bubble formation due to the low viscosity of VitroGel®. There is an option to warm the medium and hydrogel solution before mixing them, as this can help reduce the viscosity.