---
title: Shall I change the full amount of the cover medium or just partially?
description: "Changing 100% of the cover medium might disrupt the hydrogel. We recommend adding additional fresh medium without removing the top medium for the first medium change. Afterward, change 50-80% of the cover medium. (Please check the recommended volume of additional…"
url: "https://www.thewellbio.com/docs/shall-i-change-the-full-amount-of-the-cover-medium-or-just-partially/"
updated: "2024-08-19"
category: Hydrogel / Cell Preparation
tags:
  - 2D Coating
  - 3D Culture
---

# Shall I change the full amount of the cover medium or just partially?

Changing 100% of the cover medium might disrupt the hydrogel. We recommend adding additional fresh medium without removing the top medium for the first medium change. Afterward, change 50-80% of the cover medium. (Please check the recommended volume of additional cover medium in the Table below.)

![](https://cdn.thewellbio.com/wp-content/uploads/2020/06/FAQ-30-Table.png)