---
title: "What is the best technique to score the viability in intact 3D spheres?  Can Cyto3D® Live-Dead Assay Kit be used in fixed cells too or it has to be live spheroids only?"
description: "Cyto3D® Live-Dead Assay Kit is designed for cell viability testing. Software (ImageJ/Fiji) can quantify the GFP and TxRed intensity to quantify viability in culture, giving the percent of viability. The automatic cell counter or plate reader can also be used…"
url: "https://www.thewellbio.com/docs/what-is-the-best-technique-to-score-the-viability-in-intact-3d-spheres-can-cyto3d-live-dead-assay-be-used-in-fixed-cells-too-or-it-has-to-be-live-spheroids-only/"
updated: "2024-09-16"
category: Analysis (Image, DNA/RNA, Protein, etc)
tags:
  - Imaging
  - Spheroid
  - Viability
---

# What is the best technique to score the viability in intact 3D spheres?  Can Cyto3D® Live-Dead Assay Kit be used in fixed cells too or it has to be live spheroids only?

Cyto3D® Live-Dead Assay Kit is designed for cell viability testing. Software (ImageJ/Fiji) can quantify the GFP and TxRed intensity to quantify viability in culture, giving the percent of viability. The automatic cell counter or plate reader can also be used with Cyto3D® Live-Dead Assay Kit for cell suspension or 2D cell culture.