---
title: "Will Cyto3D® Live-Dead Assay Kit affect cell growth if cells are repeatedly labeled multiple times?"
description: "Cyto3D® Live-Dead Assay Kit may affect cell growth as the dye binds to the cells' nuclei. For multiple staining and long-term testing, we recommend washing the cells with PBS to remove the extra dyes after each test.    "
url: "https://www.thewellbio.com/docs/will-cyto3d-live-dead-assay-affect-cell-growth-if-repeatedly-labeling-cells-for-multiple-times/"
updated: "2024-09-17"
category: Analysis (Image, DNA/RNA, Protein, etc)
tags:
  - Imaging
---

# Will Cyto3D® Live-Dead Assay Kit affect cell growth if cells are repeatedly labeled multiple times?

Cyto3D® Live-Dead Assay Kit may affect cell growth as the dye binds to the cells' nuclei. For multiple staining and long-term testing, we recommend washing the cells with PBS to remove the extra dyes after each test.