VitroGel® LDP1 is a ready-to-use tunable hydrogel system modified with integrin-binding peptide (RGD) and laminin-derived functional peptides (IKVAV and YIGSR) at 1:1:1 ratio. The hydrogel promotes cell attachment and cell-matrix interactions and enhances cell adhesion, proliferation, motility/migration and differentiation in different applications.
Mix & Match – 3D Cell Culture Your WAY!
VitroGel® LDP1 is one of the families of the xeno-free tunable VitroGel system. This hydrogel can mix with other versions of VitroGel such as VitroGel MMP, VitroGel COL, VitroGel RGD, VitroGel IKVAV, and VitroGel YIGSR to create the customized multi-functional hydrogel with different ratio of functional ligands. Using this flexible and powerful hydrogel system, scientists get full control to manipulate the microenvironment for 3D cell culture and more!
VitroGel closely mimics the natural extracellular matrix (ECM) environment, creating a functional and optimized environment to make cells feel like at home. The hydrogel system is ready-to-use at room temperature, has a neutral pH, transparent, permeable and compatible with different imaging systems. The hydrogel strength can be adjusted with VitroGel® Dilution Solution. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.
- Cannot find the functional hydrogel you want? Contact us for a customized hydrogel system.
- Need rheology data of VitroGel with your medium and mixture for publication? Check our data support service.
- Xeno-free tunable hydrogel modified with RGD, IKVAV, and YIGSR peptides
- Support strong cell-cell communication; Good for adhesion cells or cells requiring stronger cell-matrix interactions
- Ready-to-use at room temperature
- Tunable hydrogel: Dilute with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different concentrations
- Neutral pH
- Compatible with VitroGel® Cell Recovery Solution for easy cell harvesting
- Injectable hydrogel (Check user handbook for preparation details)
- 10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa
- Ships room temperature. Store at 2-8°C
- Size: 2 mL and 10 mL
Handbook and Resources
Frequently Asked Questions
To see a full list of FAQ, click here. FAQ LIST
- How to prepare the cell suspension to mix the hydrogel? Shall I add serum?
If cells cultured in a complete cell culture medium, which is supplement with 10% FBS or other critical supplements, please prepare the cell suspension using the following methods before mixing it with the hydrogel solution.
- Prepare the cell suspension with 2X concentration (e.g. 100K), and mix with 100% FBS at 1:1 (v/v) ratio to get 1X cell suspension (50K) with 50% FBS.
- Mix the diluted hydrogel solution with the cell suspension from above at 4:1 (v/v) ratio to get the final cells in the hydrogel at 10K with 10% FBS supplement.
If serum plan is an important role in your traditional cell culture, it is also important for 2D coating and 3D culture. Adding serum supplements in the hydrogel and adjusting the final serum concentration to the target level would support cell growth in the hydrogel system.
- How do I adjust the hydrogel formation time?
– If VitroGel needs to be diluted more than 1:3 ratio, a longer waiting time (20-30 min) may be needed for soft gel formation. Using a higher volume of cell culture medium for mixing would help to accelerate the process of hydrogel formation.- If the hydrogel solidifies too fast after mixing with culture medium (showing as small solid gel chunk), adjust the mixing ratio by using less cell culture medium. For example, if mixing 4 mL diluted hydrogel solution with 1 mL cell culture medium lead to the solid gel chuck (particles), then mixing 4 mL diluted hydrogel solution with 0.5-0.8 mL cell culture medium would help to solve the issue.- On the other hand, if the hydrogel formation is too slow, which may happen when using low hydrogel concentration at 1:3 or 1:4 dilution or using cell culture medium with very low ionic concentration, adjust the mixing ratio by using more cell culture medium. For example, if mixing 4 mL diluted hydrogel solution with 1 mL cell culture medium lead to a slow hydrogel formation, then mixing 4 mL diluted hydrogel solution with 1.5-4 mL cell culture medium would help to solve the issue.
- How do I adjust the stiffness of the final hydrogel?
The stiffness of the final hydrogel can be adjusted by diluting the hydrogel solution before mixing it with cell culture media. Our VitroGel® Dilution Solution can help to adjust the hydrogel concentration. Please read the “First-time User Note” to learn how to prepare different VitroGel dilutions. If you need a higher hydrogel stiffness than the original product, please contact us at firstname.lastname@example.org.
- Can I harvest cells from the hydrogel after 3D culture?
Yes, the cells can be harvested after 2D coating or 3D culture by using the VitroGel Cell Recovery Solution. VitroGel® Cell Recovery Solution is a ready-to-use, enzyme-free solution to harvest 2D or 3D cultured cells from hydrogel fast and safely. The solution is compatible with the VitroGel hydrogel system and can recover cells from VitroGel in 15 minutes. VitroGel Cell Recovery Solution is room temperature stable, has a neutral pH and works at 37 °C operating temperature. The solution can maintain high cell viability during the recovery process. Cells can be sub-culture in both 2D and 3D culture after recovery.
Data and References
Figure 1. Rheological properties of VitroGel LDP1 with DMEM medium. A) The gel formation curve after mixing with DMEM medium. VitroGel LDP1 was diluted at 1:0,1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with DMEM at 4:1 (v/v) ratio; B) The gel strength after 24 hrs incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa)
Figure 2. 3D culture of mouse bone marrow stromal cells (op9, mesenchyme) in the mixture of VitroGel LDP1. Cells was cultured with 1:3 diluted VitroGel LDP1 following the user handbook (50% FBS was used to prepared cell suspension to get hydrogel with final 10% FBS concentration).
Figure 3. 3D culture of glioblastoma cells (U-87 MG) in VitroGel LDP1. Cells was cultured with 1:3 diluted VitroGel LDP1 follow the user handbook (50% FBS was used to prepared cell suspension to get hydrogel with final 10% FBS concentration) .
- Shen, S., Dean, D. C., Yu, Z., Hornicek, F., Kan, Q., & Duan, Z. (2020). Aberrant CDK9 expression within chordoma tissues and the therapeutic potential of a selective CDK9 inhibitor LDC000067. Journal of Cancer, 11(1), 132–141. https://doi.org/10.7150/jca.35426
- Shamloo, B., Kumar, N., Owen, R. H., Reemmer, J., Ost, J., Perkins, R. S., & Shen, H.-Y. (2019). Dysregulation of adenosine kinase isoforms in breast cancer. Oncotarget, 10(68). https://doi.org/10.18632/oncotarget.27364
- Wang, F., Nan, L., Zhou, S., Liu, Y., Wang, Z., Wang, J., Feng, X., & Zhang, L. (2019). Injectable Hydrogel Combined with Nucleus Pulposus-Derived Mesenchymal Stem Cells for the Treatment of Degenerative Intervertebral Disc in Rats. Stem Cells International, 2019, 1–17. https://doi.org/10.1155/2019/8496025
- Borzi, C., Calzolari, L., Ferretti, A. M., Caleca, L., Pastorino, U., Sozzi, G., & Fortunato, O. (2019).c-Myc shuttled by tumour-derived extracellular vesicles promotes lung bronchial cell proliferation through miR-19b and miR-92a. Cell Death & Disease, 10(10). https://doi.org/10.1038/s41419-019-2003-5
- Kim, E. J., Yang, C., Lee, J., Youm, H. W., Lee, J. R., Suh, C. S., & Kim, S. H. (2019). The new biocompatible material for mouse ovarian follicle development in three-dimensional in vitro culture systems. Theriogenology. https://doi.org/10.1016/j.theriogenology.2019.12.009
- Di Donato, M., Cernera, G., Migliaccio, A., & Castoria, G. (2019). Nerve Growth Factor Induces Proliferation and Aggressiveness in Prostate Cancer Cells. Cancers, 11(6), 784. https://doi.org/10.3390/cancers11060784
- Xiao, M., Qiu, J., Kuang, R., Zhang, B., Wang, W., & Yu, Q. (2019). Synergistic effects of stromal cell-derived factor-1α and bone morphogenetic protein-2 treatment on odontogenic differentiation of human stem cells from apical papilla cultured in the VitroGel 3D system. Cell and Tissue Research, 378(2), 207–220. https://doi.org/10.1007/s00441-019-03045-3
- Thanindratarn, P., Li, X., Dean, D. C., Nelson, S. D., Hornicek, F. J., & Duan, Z. (2019). Establishment and Characterization of a Recurrent Osteosarcoma Cell Line: OSA 1777. Journal of Orthopaedic Research. https://doi.org/10.1002/jor.24528
- Li X, Seebacher NA, Xiao T, Hornicek FJ, Duan Z. Targeting regulation of cyclin dependent kinase 9 as a novel therapeutic strategy in synovial sarcoma. Journal of Orthopaedic Research®, 37(2), 510–521. https://doi.org/10.1002/jor.24189
- Hangzhan M, Nicole AS, Francis JH, Zhenfeng D. Cyclin-dependent kinase 9 (CDK9) is a novel prognostic marker and therapeutic target in osteosarcoma. EBioMedicine, 39, 182–193. https://doi.org/10.1016/j.ebiom.2018.12.022
- Akamandisa MP, Nie K, Nahta R, Hambardzumyan D, Castellino RC. Inhibition of mutant PPM1D enhances DNA damage response and growth suppressive effects of ionizing radiation in diffuse intrinsic pontine glioma. Neuro-Oncology, 21(6), 786–799. https://doi.org/10.1093
- Huang J. 3D Cell Culture on VitroGel System. HSOA Journal of Cytology and Tissue Biology. https://doi.org/10.24966/CTB-9107/S1001
- Mahauad-Fernandez WD, Okeoma CM. B49, a BST-2-based peptide, inhibits adhesion and growth of breast cancer cells. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-22364-z
- Mahauad-Fernandez WD, Naushad W, Panzner TD, Bashir A, Lal G, Okeoma CM. BST-2 promotes survival in circulation and pulmonary metastatic seeding of breast cancer cells. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-35710-y
- Seungwoo S, Jihye K, Je-Ryung L, Eun-chae J, Tae-Jin J, Wonhee L, YongKeun P. Enhancement of optical resolution in three-dimensional refractive-index tomograms of biological samples by employing micromirror-embedded coverslips. Lab on a Chip, 18(22), 3484–3491. https://doi.org/10.1039/c8lc00880a
- Powell K. Adding depth to cell culture. Science, 356(6333), 96–98. https://doi.org/10.1126/science.356.6333.96