VitroINK™ 3D is a ready-to-use tunable bioink for 3D bioprinting. The pure hydrogel matrix structure is good for cell spheroid formation and supports the growth of cells requiring low cell-matrix interactions.
VitroINK is a family of the ready-to-use, xeno-free tunable bioink system that requires no UV, no temperature/pH curing, or chemical cross-linking. The system is ready-to-use at room temperature, neutral in pH, transparent, and has excellent visibility after printing and cell culture. Due to the unique shear-thinning and rapid recovery mechanical property, VitroINK can maintain the printed structure without UV or other special curing methods. Adding cell culture medium after printing can further stabilize the printed structure and support cell growth. Cells can be pre-mix with VitroINK using our VitroINK Mixing Kit for mixing ratios at 3:1 or 10:1. Different versions of VItroINK may incorporate multiple biological functional ligands to promote cell attachment, cell-matrix interactions, cell proliferation, motility/migration and differentiation for many different applications.
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- Need rheology data of VitroINK with your medium and mixture for publication? Check our data support service.
- Xeno-free tunable bioink for 3D bioprinting
- Ready-to-use at room temperature
- No UV, temperature/pH curing or chemical cross-linking required
- Neutral pH
- Transparent. Excellent visibility after printing and cell culture
- Pre-mix with cells by using our VitroINK Mixing Kit
- Ships room temperature. Store at 2-8°C
- Sizes: 3 mL and 10 mL
Handbook and Resources
Data and References
Figure 1. Rheological properties of VitroINK 3D under the time sweep and temperature sweep tests. A) The time sweep test of VitroINK 3D without or with 3:1 and 10:1 (v/v) mixing with DMEM medium. The elastic modulus was tested at room temperature and showed a feasible range for extrusion printing before incubation with cell culture medium. B) the elastic modulus and viscosity properties of VitroINK 3D under the temperature sweep testing (20 to 40°C). The elastic modulus and viscosity are relatively stable at 20 to 36°C. When temperatures higher than 36, the elastic modulus and viscosity decrease. The data indicate the VitroINK has a wide range of working temperatures and can be easily used at room temperature or physiological temperature.
Figure 2. Shear-thinning and rapid recovery property of VitroINK 3D. A) The shear-thinning property of VitroINK under the shear rate from 0.1 to 100 HZ. B) the amplitude sweep test shows the shear thinning and rapid recovery property of VitroINK under the shear strain change from 0.1 to 500% in two circles. With this unique shear-thinning and rapid recovery mechanical property, VitroINK can well maintain the printed structure after extrusion.
Figure 3. Gel strength of VitroINK 3D after adding cell culture medium and overnight incubation. The VitroINK 3D was mixed with the cell culture medium at different ratio and submerged to the medium for overnight incubation, which can further stabilize the printed structure and support cell growth.
Figure 4. The printed line width of VitroINK 3D with 22G, 25G, 27G nozzle at different pressures and print speeds. The line width decreases as the print speed increases. At lower printing pressures, lines can only be printed continuously under low print speed. (VitroINK was printed by using Cellink INKCREDIBLE+ 3D bioprinter)
Figure 5. POI value of VitroINK 3D with 22G, 25G, 27G nozzle at different pressures and print speeds. For all three nozzle sides, the print speed at 3 mm/s gives the highest POI value at low pressure. Under a fixed pressure, the faster print speed gives a higher POI value.
Figure 6. Adjusted printing conditions after mixing VitroINK with Cell culture medium. Mixing at 3:1 (A) or 10:1 (B) ratios by using VitroINK mixing kit. The line width increase after mixing. Adjust the printing speed from 3 to 7 or 5 mm/s can bring the line width down.
Figure 7. Glioblastoma cell line (SNB 75) cultured in VitroINK 3D (3:1 bioink: cells mixing) at day 4.