VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs). The Bead-Droplet method can be used to make hydrogel cell beads for MSC scale-up and long-term culture. This novel culture method generates high-quality MSCs and eliminates the usage of microcarriers for MSC scale-up.