Xeno-free and biofunctional
100% synthetic. Animal & human origin-free hydrogel. No growth factors or proteins in the gel solution.
VitroGel® NEURON
Ready-to-use, xeno-free hydrogel system for neural stem cell and neuron cultures.
VitroGel® NEURON for 2D and 3D neuronal growth
Room temperature operation
Stable at room temperature. Easy handling. Gelation by mixing; not temperature dependent.
Supports NSC and neuron cultures
Suitable for 3D and 2D culture of immortalized neuronal neuroblasts and iPSC-derived NSCs and neurons.
Efficient for single cell and neurosphere cultures
Supports culturing of neural stem cells (NSCs) as single cells or neurospheres.
VitroGel® NEURON hydrogel is a synthetic matrix with functional ligands that support the culture of neuronal neuroblasts, mature neurons, and iPSC-derived neural stem cell (NSC) maintenance and differentiation. The hydrogel can be used for 2D and 3D cell culture applications.
VitroGel® NEURON hydrogel is a ready-to-use, xeno-free, transparent, and room temperature stable system, compatible with imaging systems and suitable for laboratory automation and clinical applications. VitroGel® NEURON hydrogel polymerizes once the solution is combined with the medium. Growth factors can be mixed with the matrix or added on top of the gel to support NSC cultures.
3D neuron culture process in 30 min – “Just add cells”
VitroGel® NEURON is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.
Specifications
| Formulation | Xeno-free, biofunctional hydrogel |
| Use | 3D and 2D neuron and neural stem cell culture |
| Operation | Ready-to-use at room temperature |
| Injection | Injectable hydrogel for in vivo studies and lab automation |
| pH | Neutral |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Sizes | 10 mL and 2 mL |
| Complementary Products | Cell Harvesting VitroGel® Organoid Recovery Solution 10-15 min cell recovery Cell Viability Cyto3D® Live-Dead Assay Kit Fast, sensitive |
Protocols and Resources
Product Data Sheet
Product Data Sheet
Data and References
2D thin coating method
2D NSC Maintenance
Figure 1. VitroGel® NEURON used as a thin 2D coating on VitroPrime™ Spread-Attach Plate for Growth of IPSC-derived NSCs. CD34-eIPSC-NSC cells were plated onto (50,000 cells per well) a 24-well, VitroPrime™ Spread-Attach Plate coated with VitroGel® NEURON 1:200, or Geltrex, and grown for 6 days while being analyzed using an Incucyte system (S3). Pictures captured using Incucyte system, and Brightfield images were enhanced using HDR and level function on Photoshop in order to to highlight the contrast of the cells.
2D hydrogel covering “blanket” method
2D Neuronal Differentiation of B35 cell line
Figure 2: 2D “Blanket” method using VitroGel® NEURON hydrogel sustains in vitro neuronal differentiation. Immunofluorescence staining of neuronal cultures was performed to evaluate the presence of the neuron-associated marker beta-III-tubulin on days 7, 14, 21, and 30 post-differentiation induction. A. Light microscopy image showing neuronal cultures. B. Green-fluorescent image illustrating the presence of beta-III-tubulin. C. The nuclei were observed using DAPI (blue) staining. D. Combination of B and C images. The cultures were visualized using Molecular Devices Image Xpress Nano system at a 20X magnification.
3D Cell Culture
3D Cell Culture of B35 Cell Lines
Day
7
Day
14
Day
21
Figure 3. VitroGel® NEURON sustains long-term 3D neuronal differentiation.
Immunofluorescence staining of neuronal cultures for the neuron-specific marker beta-III-tubulin on days 7,14, and 21 post-differentiation induction. Images represent the following: A. Light microscopy image of neuronal cultures. B. Beta-III-tubulin presence, indicative of positive neuronal differentiation. C. Nuclei staining using DAPI (blue). D. Merged images of B and C. E. Merged images of A, B, and C. Images were obtained using Image Xpress Nano Imaging System from Molecular Devices at a 20X magnification.
| Size | 2 mL, 10 mL |
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