Category Archives: Application Notes

Using the VitroGel Hydrogel System as a Versatile Platform for Invasion Assay

Application Note Nana-Fatima Haruna, Atsuo Ochi, John Huang TheWell Bioscience, North Brunswick, NJ 08902 Introduction Invasion studies have been crucial in identifying key intracellular signaling pathways and membrane-bound and soluble enzymes that influence cellular movement through a three dimensional (3D) extracellular matrix (ECM)1,2. Such information has been used to investigate mechanisms governing the infiltration of […]

3D Printed Tumor Spheroids for Disease Modeling and Chemotherapeutic Drug Screening

Poster Presentation TheWell Bioscience Inc. Aragaw Gebeyehu1, Sunil Surapaneni1, John Huang2, Arindam Mondal1, Vivian Wang2, Nana-Fatima Haruna2, Peggy Arthur1, Shallu Kutlehria1, Nil Patel1, Mandip Sachdeva1 1College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL3230; 2TheWell Bioscience, Inc., North Brunswick NJ 08902 Abstract 3D bioprinting is intended for regenerative medicine and disease model development […]

Characterizing the Biophysical Properties of a Tunable Hydrogel System for 3D Cell Culture

Poster Presentation TheWell Bioscience Inc. Nana Fatima Haruna, Ziwen Vivian Wang, John Huang TheWell Bioscience, Inc., North Brunswick NJ 08902 Abstract The hydrogel-based 3D cell culture model more accurately depicts the microenvironment of cells in vivo than the traditional 2D cell culture system, which enables cells to grow, develop, operate, and produce feedback as if […]

3D Cell Culture of Glioblastoma Cell (U-87 MG) in VitroGel® Hydrogel System

Application Note TheWell Bioscience Inc. Introduction Glioblastoma is an aggressive type of cancer that occurs in the brain or spinal cord. Over the years, various models of glioblastoma have been developed to help understand the mechanism initiation and progression1,2. However, this has been complicated by the diverse and complex genetic and environmental factors that promote […]

3D Cell Culture of Bone Marrow Stroma Cells (OP9) on VitroGel® Hydrogel System

Application Note TheWell Bioscience Inc. Introduction Mesenchymal stem cells are a type of adult multipotent stem cell that gives rise to bone, cartilage, adipose, and connective tissue. Over the last two decades, they have become a wildly popular potential alternative to embryonic stem cells for regenerative medicine because of their broad plasticity, immunomodulatory, and anti-inflammatory […]

Long-Term Neuron Culture Maturation in 3D Hydrogel Constructs

A comparison study of VitroGel® and Matrigel® for long-term neuron maturation and survival in vitro Comparison Study Martin L. Tomov Stanley Center; Broad Institute of MIT and Harvard Introduction Three dimensional (3D) tissue cultures have numerous advantages compared to traditional two dimensional (2D) cultures [1; 5]. Minimizing exposure to hard surfaces can improve cell maturation […]

3D Cell Culture of Human Colon Cancer Cells (HCT116) on VitroGel® System

Application Note TheWell Bioscience Inc. Introduction In vitro assays have become increasingly popular in drug discovery applications because of their high throughput functionality and cost-effectiveness in conducting optimization and preclinical validation. Three-dimensional (3D) culture assays have become especially popular because compared to traditional two-dimensional (2D) cell culture, 3D culture provides a more physiologically relevant environment […]

3D Cell Culture of Human Pancreatic Cancer Cells (PANC-1) on VitroGel® System

Application Note TheWell Bioscience Inc. Introduction 3D culture has become the standard for studying complex systems, such as the tumor microenvironment (TME), in an in vitro setting. These systems allow for insight into the cell-cell interactions and communications that occur in vivo. Use of 3D matrices mimic the complex TME (2) and are even beginning […]