Category Archives: Application Notes

3D Cell Culture of Glioblastoma Cell (U-87 MG) in VitroGel® Hydrogel System

Application Note TheWell Bioscience Inc. Introduction Glioblastoma is an aggressive type of cancer that occurs in the brain or spinal cord. Over the years, various models of glioblastoma have been developed to help understand the mechanism initiation and progression1,2. However, this has been complicated by the diverse and complex genetic and environmental factors that promote […]

3D Cell Culture of Bone Marrow Stroma Cells (OP9) on VitroGel® Hydrogel System

Application Note TheWell Bioscience Inc. Introduction Mesenchymal stem cells are a type of adult multipotent stem cell that gives rise to bone, cartilage, adipose, and connective tissue. Over the last two decades, they have become a wildly popular potential alternative to embryonic stem cells for regenerative medicine because of their broad plasticity, immunomodulatory, and anti-inflammatory […]

Long-Term Neuron Culture Maturation in 3D Hydrogel Constructs

A comparison study of VitroGel™ and Matrigel® for long-term neuron maturation and survival in vitro Comparison Study Martin L. TomovStanley Center; Broad Institute of MIT and Harvard Introduction Three dimensional (3D) tissue cultures have numerous advantages compared to traditional two dimensional (2D) cultures [1; 5]. Minimizing exposure to hard surfaces can improve cell maturation and […]

3D Cell Culture of Human Colon Cancer Cells (HCT116) on VitroGel™ System

Introduction In vitro assays have become increasingly popular in drug discovery applications because of their high throughput functionality and cost-effectiveness in conducting optimization and preclinical validation. Three-dimensional (3D) culture assays have become especially popular because compared to traditional two-dimensional (2D) cell culture, 3D culture provides a more physiologically relevant environment (1-4). Comparative studies have shown […]

3D Cell Culture of Human Pancreatic Cancer Cells (PANC-1) on VitroGel™ System

Introduction 3D culture has become the standard for studying complex systems, such as the tumor microenvironment (TME), in an in vitro setting. These systems allow for insight into the cell-cell interactions and communications that occur in vivo. Use of 3D matrices mimic the complex TME (2) and are even beginning to mimic metastases (3), by […]