VitroGel® MMP

Matrix metalloproteinases (MMP) sensitive biodegradable hydrogel – xeno-free, tunable, high concentration

  • 2 mL
  • 10 mL
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Overview

VitroGel® MMP is a ready-to-use tunable hydrogel system modified with matrix metalloproteinases sensitive peptide. The enzyme sensitive hydrogel can be degraded by several MMPs (MMP1, MMP2, MMP3, MMP9 and MMP13) and support different biological activities such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, etc.

Mix & Match – 3D Cell Culture Your WAY!

VitroGel® MMP is one of the families of the xeno-free tunable VitroGel system. This hydrogel can mix with other versions of VitroGel such as VitroGel® RGD, VitroGel® IKVAV, VitroGel® YIGSR, and VitroGel® COL to create the customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists get full control to manipulate the micro-environment for 3D cell culture and more!

VitroGel closely mimics the natural extracellular matrix (ECM) environment, creating a functional and optimized environment to make cells feel like at home. The hydrogel system is ready-to-use at room temperature, has a neutral pH, transparent, permeable and compatible with different imaging systems. The hydrogel strength can be adjusted with VitroGel® Dilution Solution. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.

  • Cannot find the functional hydrogel you want? Contact us for a customized hydrogel system.
  • Need rheology data of VitroGel with your medium and mixture for publication? Check our data support service.

Specifications

  • Xeno-free tunable hydrogel modified with matrix metalloproteinases sensitive peptide
  • MMP sensitive biodegradable hydrogel
  • Ready-to-use at room temperature
  • Tunable hydrogel:  Dilute with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different concentrations
  • Neutral pH
  • Transparent
  • Compatible with VitroGel® Cell Recovery Solution for easy cell harvesting
  • Injectable hydrogel (Check user handbook for preparation details)
  • Mix and match with other versions of VitroGel hydrogel for multifunctional hydrogel
  • 10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa
  • Ships room temperature. Store at 2-8°C
  • Size: 2 mL and 10 mL

Handbook and Resources

Product Documentation

Frequently Asked Questions

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To see a full list of FAQ, click here.  FAQ LIST

  • How to prepare the cell suspension to mix the hydrogel? Shall I add serum?
    If cells cultured in a complete cell culture medium, which is supplement with 10% FBS or other critical supplements, please prepare the cell suspension using the following methods before mixing it with the hydrogel solution.

    1. Prepare the cell suspension with 2X concentration (e.g. 100K), and mix with 100% FBS at 1:1 (v/v) ratio to get 1X cell suspension (50K) with 50% FBS.
    2. Mix the diluted hydrogel solution with the cell suspension from above at 4:1 (v/v) ratio to get the final cells in the hydrogel at 10K with 10% FBS supplement.

    If serum plan is an important role in your traditional cell culture, it is also important for 2D coating and 3D culture. Adding serum supplements in the hydrogel and adjusting the final serum concentration to the target level would support cell growth in the hydrogel system.

  • How do I adjust the hydrogel formation time?

    –  If VitroGel needs to be diluted more than 1:3 ratio, a longer waiting time (20-30 min) may be needed for soft gel formation. Using a higher volume of cell culture medium for mixing would help to accelerate the process of hydrogel formation.-  If the hydrogel solidifies too fast after mixing with culture medium (showing as small solid gel chunk), adjust the mixing ratio by using less cell culture medium. For example, if mixing 4 mL diluted hydrogel solution with 1 mL cell culture medium lead to the solid gel chuck (particles), then mixing 4 mL diluted hydrogel solution with 0.5-0.8 mL cell culture medium would help to solve the issue.-  On the other hand, if the hydrogel formation is too slow, which may happen when using low hydrogel concentration at 1:3 or 1:4 dilution or using cell culture medium with very low ionic concentration, adjust the mixing ratio by using more cell culture medium. For example, if mixing 4 mL diluted hydrogel solution with 1 mL cell culture medium lead to a slow hydrogel formation, then mixing 4 mL diluted hydrogel solution with 1.5-4 mL cell culture medium would help to solve the issue.
  •  How do I adjust the stiffness of the final hydrogel?
    The stiffness of the final hydrogel can be adjusted by diluting the hydrogel solution before mixing it with cell culture media. Our VitroGel® Dilution Solution can help to adjust the hydrogel concentration. Please read the “First-time User Note” to learn how to prepare different VitroGel dilutions. If you need a higher hydrogel stiffness than the original product, please contact us at support@thewellbio.com.

  • Can I harvest cells from the hydrogel after 3D culture?
    Yes, the cells can be harvested after 2D coating or 3D culture by using the VitroGel Cell Recovery Solution. VitroGel® Cell Recovery Solution is a ready-to-use, enzyme-free solution to harvest 2D or 3D cultured cells from hydrogel fast and safely. The solution is compatible with the VitroGel hydrogel system and can recover cells from VitroGel in 15 minutes. VitroGel Cell Recovery Solution is room temperature stable, has a neutral pH and works at 37 °C operating temperature. The solution can maintain high cell viability during the recovery process. Cells can be sub-culture in both 2D and 3D culture after recovery.

SEE MORE FAQ (Frequently Asked Questions)

Video Resources

Data and References

Figure 1.  Rheological properties of VitroGel MMP with RPMI medium. A) The gel formation curve after mixing with RPMI medium. VitroGel MMP was diluted at 1:1 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with RPMI at 4:1 (v/v) ratio; B) The gel strength after 24 hrs incubation. The hydrogel was prepared according to the user handbook at 1:0, 1:1 and 1:3 dilution ratios, and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa)

Figure 2.  Degradation of VitroGel MMP gel with MMP2 enzyme. A) The hydrogel was prepared without dilution (1:0 dilution) and incubated for 24 hrs before adding MMP2 (1 µg/mL) on the top of the hydrogel. The plate was incubated in a 5% CO2 incubator at 37°C and was shaken using an orbital shaker at 40 rpm. The hydrogel appearance starts getting chunky after 24 hrs and then by Day 6, the entire middle section has degraded; B) The 1:3 diluted hydrogel was prepared with VitroGel Dilution Solution and incubated as 1:0 diluted hydrogel. The hydrogel appearance shows high degradation after 6 hrs after exposed to MMP2 enzyme. C). The rheological data shows a rapid decline in the elastic modulus of the 1:3 diluted hydrogel overtime after adding MMP2 enzyme. The gel was almost complete degraded by the enzyme after 200 mins. The test result demonstrates that VitroGel MMP undergoes an MMP-sensitive degradation. The higher the dilution of the gel, the faster the degradation process.

Figure 3. 3D culture of glioblastoma cells (SNB 75) in VitroGel MMP. Cells were cultured with 1:3 diluted VitroGel IKVAV according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).

Mix & Match Hydrogel System – 3D Cell Culture Your Way
Mix VitroGel RGD with VitroGel MMP

Figure 4. 3D culture of mouse bone marrow stromal cells (op9, mesenchyme) in the mixture of VitroGel RGD and VitroGel MMP. The mixing ratios of VitroGel RGD and VitroGel MMP is 3:1 (V/V). Cells were cultured with 1:3 diluted VitroGel mixture according to the user handbook (50% FBS was used to prepare cell suspension to achieve hydrogel with a final 10% FBS concentration).

References/Publications

Size

2 mL, 10 mL