Xeno-free and biofunctional
100% synthetic. Animal & human origin-free hydrogel. No growth factors or proteins in the gel solution.
VitroGel® NEURON
Ready-to-use, xeno-free hydrogel system for neural stem cell and neuron cultures.
VitroGel® NEURON for 2D and 3D neuronal growth
Room temperature operation
Stable at room temperature. Easy handling. Gelation is not temperature dependent. Learn how gelation works >
Supports 3D and 2D neuronal cell culture
Suitable for 3D and 2D culture of immortalized neuronal neuroblasts, iPSC-derived NSCs, neuron, and culturing of neural stem cells (NSCs) as single cells or neurospheres.
Injectable hydrogel for cell therapy
VitroGel® NEURON’s unique injectable properties make it an injectable carrier for neuron-based cell therapy, spinal cord injury repair, and other regenerative medicine applications.
VitroGel® NEURON hydrogel is a synthetic matrix with functional ligands that support the culture of neuronal neuroblasts, mature neurons, and iPSC-derived neural stem cell (NSC) maintenance and differentiation. The hydrogel can be used for 2D and 3D cell culture applications.
VitroGel® NEURON hydrogel is a ready-to-use, xeno-free, transparent, and room temperature stable system, compatible with imaging systems and suitable for laboratory automation and clinical applications. With its user-friendly, ready-to-use formulation, scientists can seamlessly transition between 2D and 3D cultures without the need for animal-derived components, significantly improving experimental consistency and translational relevance.
VitroGel® NEURON hydrogel polymerizes once the solution is combined with the medium. Growth factors can be mixed with the matrix or added on top of the gel to support NSC cultures.
3D neuron culture process in 30 min – “Just add cells”
VitroGel® NEURON is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.
Specifications
| Formulation | Xeno-free, biofunctional hydrogel |
| Use | 3D and 2D neuron and neural stem cell culture |
| Operation | Ready-to-use at room temperature |
| Injection | Injectable hydrogel for in vivo studies and lab automation |
| pH | Neutral |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Sizes | 10 mL and 2 mL |
Recommended Product
Premium quality cell culture plate with unique surface treatment for superior hydrogel spreading, adherence, and uniform surface.
Protocols and Resources
Material Safety Data Sheet (MSDS)Data and References
2D thin coating method
Advantages of using VitroGel® NEURON for 2D Thin Coating
4X more usage compared to animal-based ECM
VitroGel® NEURON enables thin, even coating at just 1:200 dilution, outperforming typical 1:50–1:100 dilution of animal-based ECMs.
30 minutes.
Easy and Fast Operation
VitroGel® NEURON allows a simple and quick workflow. No need to remove the coating solution, just seed the cells directly. This shortens operating time and minimizes operating errors.
Over 40% savings on consumables. No PDL-coated plates required.
For best results, use with VitroPrime™ Spread-Attach Plate to achieve uniform coating and rapid cell growth. No need for expensive PDL-coated plates, reducing consumable costs.
2D NSC Maintenance
Figure 1: Time-Lapse Growth of NSCs over 6 days on VitroPrime™ Spread-Attach Plates.
Incucyte S3 Time-Lapse Growth of NSCs over 6 days on VitroPrime™ Spread-Attach Plates. NSCs plated on 24-well, VitroPrime™ Spread-Attach Plates were seeded with 50,000 cells per well and placed into an Incucyte S3 for observation every 8 hours over the course of 6 days. Images were collected and analyzed for confluence.
Figure 2. VitroGel® NEURON used as a thin 2D coating on VitroPrime™ Spread-Attach Plate for Growth of IPSC-derived NSCs. CD34-eIPSC-NSC cells were plated onto (50,000 cells per well) a 24-well, VitroPrime™ Spread-Attach Plate coated with VitroGel® NEURON 1:200, or Geltrex, and grown for 6 days while being analyzed using an Incucyte system (S3). Pictures captured using Incucyte system, and Brightfield images were enhanced using HDR and level function on Photoshop in order to highlight the contrast of the cells.
Figure 3: Growth Curve of NSCs on 24-well VitroPrime™ Spread-Attach Plates.
Percent Confluence of NSCs over 6 days on VitroPrime™ Spread-Attach Plates. NSCs plated on 24-well, VitroPrime™ Spread-Attach Plates were seeded with 50,000 cells per well and placed into an Incucyte S3 for observation every
8 hours over the course of 6 days. Images were collected, analyzed for confluence by comparing to initial image atTime = 0 (+4hrs).
2D hydrogel covering “blanket” method
2D Neuronal Differentiation of B35 cell line
Figure 4: 2D “Blanket” method using VitroGel® NEURON hydrogel sustains in vitro neuronal differentiation. Immunofluorescence staining of neuronal cultures was performed to evaluate the presence of the neuron-associated marker beta-III-tubulin on days 7, 14, 21, and 30 post-differentiation induction. A. Light microscopy image showing neuronal cultures. B. Green-fluorescent image illustrating the presence of beta-III-tubulin. C. The nuclei were observed using DAPI (blue) staining. D. Combination of B and C images. The cultures were visualized using Molecular Devices Image Xpress Nano system at a 20X magnification.
3D Cell Culture of B35 Cell Lines
Day
7
Day
14
Day
21
Figure 5. VitroGel® NEURON sustains long-term 3D neuronal differentiation.
Immunofluorescence staining of neuronal cultures for the neuron-specific marker beta-III-tubulin on days 7,14, and 21 post-differentiation induction. Images represent the following: A. Light microscopy image of neuronal cultures. B. Beta-III-tubulin presence, indicative of positive neuronal differentiation. C. Nuclei staining using DAPI (blue). D. Merged images of B and C, and A for Day 14. Images were obtained using Image Xpress Nano Imaging System from Molecular Devices at a 20X magnification.
Enhance your neuron cell culture with these products:
New
Cell Culture Plates
Unique surface treated for superior hydrogel spreading, adherence, and uniform surface.
CytoGrow - Growth Factors
Premium CytoGrow™ growth factor suitable for cell culture supplementation, wound healing research, epithelial biology, and cancer signaling studies.
Cell Harvesting Solution
Non-enzymatic cell harvesting solution to recover cells/organoids from hydrogel or an animal-based ECM within 15 minutes. Improved formulation over VitroGel® Cell Recovery Solution.
Biomarkers
$238.00
Versatile, live/dead cell viability analysis for 3D and 2D cell culture. IN STOCK
| Size | 2 mL, 10 mL |
|---|
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