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VitroGel® RGD High Concentration
RGD modified – tunable, xeno-free hydrogel – high concentration (3 mL kit)
$585.00
Overview
VitroGel® RGD High Concentration is a tunable, xeno-free hydrogel system modified with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. VitroGel RGD High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.
Mix & Match – 3D Cell Culture Your WAY!
Unique to VitroGel High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel® RGD High Concentration can be “mix & matched” with other VitroGel High Concentration hydrogels such as VitroGel® IKVAV, VitroGel® YIGSR, VitroGel® MMP, and VitroGel® COL to create a customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists customize their 3D culture micro-environment for different applications.
Specifications
| Contents | VitroGel® RGD High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
| Hydrogel Formulation | Xeno-free tunable hydrogel modified with RGD peptide. HIGH CONCENTRATION |
| Use | Good for adhesion cells or cells requiring stronger cell-matrix interactions. |
| Mix & Match | Can be blended with other versions of VitroGel to create a multi-functional hydrogel |
| Operation | Room temperature |
| Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
| pH | Neutral |
| Color | Transparent |
| Cell Harvesting | 20 min cell recovery using VitroGel Cell Recovery Solution |
| Injectable | Injectable hydrogel |
| Storage | Store at 2-8°C. Ships at ambient temperature |
3D cell culture process in 20 min
VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.
Tunable Hydrogel Strength
Simply diluting the hydrogel controls the gel strength
Handbooks and Resources
Product Documentation
DOCUMENT
Technical Data Sheet
Frequently Asked Questions
View FAQ (Expand)
To see a full list of FAQ, click here. FAQ LIST
- How to prepare the cell suspension to mix the hydrogel? Shall I add serum?
If cells cultured in a complete cell culture medium, which is supplement with 10% FBS or other critical supplements, please prepare the cell suspension using the following methods before mixing it with hydrogel solution.
- Prepare the cell suspension with 2X concentration (e.g. 100K), and mix with 100% FBS at 1:1 (v/v) ratio to get 1X cell suspension (50K) with 50% FBS.
- Mix the diluted hydrogel solution with the cell suspension from above at 4:1 (v/v) ratio to get the final cells in the hydrogel at 10K with 10% FBS supplement.
If serum plan is an important role in your traditional cell culture, it is also important for 2D coating and 3D culture. Adding serum supplements in the hydrogel and adjusting the final serum concentration to the target level would support cell growth in the hydrogel system.
- How do I adjust the hydrogel formation time?
– If VitroGel needs to be diluted more than 1:3 ratio, a longer waiting time (20-30 min) may be needed for soft gel formation. Using a higher volume of cell culture medium for mixing would help to accelerate the process of hydrogel formation.- If the hydrogel solidifies too fast after mixing with culture medium (showing as small solid gel chunk), adjust the mixing ratio by using less cell culture medium. For example, if mixing 4 mL diluted hydrogel solution with 1 mL cell culture medium lead to the solid gel chuck (particles), then mixing 4 mL diluted hydrogel solution with 0.5-0.8 mL cell culture medium would help to solve the issue.- On the other hand, if the hydrogel formation is too slow, which may happen when using low hydrogel concentration at 1:3 or 1:4 dilution or using a cell culture medium with very low ionic concentration, adjust the mixing ratio by using more cell culture medium. For example, if mixing 4 mL diluted hydrogel solution with 1 mL cell culture medium lead to a slow hydrogel formation, then mixing 4 mL diluted hydrogel solution with 1.5-4 mL cell culture medium would help to solve the issue. - How do I adjust the stiffness of the final hydrogel?
The stiffness of the final hydrogel can be adjusted by diluting the hydrogel solution before mixing with cell culture media. Our VitroGel Dilution Solution can help to adjust the hydrogel concentration. Please read the “First-time User Note” to learn how to prepare different VitroGel dilutions. If you need a higher hydrogel stiffness than the original product, please contact us at support@thewellbio.com.
- Can I harvest cells from the hydrogel after 3D culture?
Yes, the cells can be harvested after 2D coating or 3D culture by using the VitroGel Cell Recovery Solution. VitroGel™ Cell Recovery Solution is a ready-to-use, enzyme-free solution to harvest 2D or 3D cultured cells from hydrogel fast and safely. The solution is compatible with the VitroGel hydrogel system and can recover cells from VitroGel in 15 minutes. VitroGel Cell Recovery Solution is room temperature stable, has a neutral pH and works at 37 °C operating temperature. The solution can maintain high cell viability during the recovery process. Cells can be sub-culture in both 2D and 3D culture after recovery.
Resources
Application Notes
Data and References
Cell Type Behavior Reference Table for VitroGel RGD
Multiple studies have made use of RGD hydrogel in different tissue and cell types. RGD is commonly used as an immobilized, adhesive ligand in 3D hydrogels that allows researcher to study many different cellular processes and behaviors in normal physiological and pathological contexts.
Bone
| Cell Type | Behavior |
|---|---|
| Goat bone marrow stromal cells | Promoted osteogenic differentiation |
| Rat bone marrow stromal cells | Promoted osteogenic differentiation |
| Rat osteoblasts | Increased cell attachment and spreading |
Cancer/Tumor
| Cell Type | Behavior |
|---|---|
| Biphasic synovial sarcoma SYO-1 | Cell proliferation and cell matrix interactions |
| Bone OSA 1777 | Cell proliferation and cell matrix interactions |
| Breast 4T1 | Cell proliferation, division, migration, and invasion |
| Breast AU-565 | Cell proliferation and cell matrix interactions |
| Breast Cancer MCF-7 | Cell proliferation, intercellular connections |
| Breast E0771 | Cell proliferation and cell matrix interactions |
| Breast MDA-MB-231 | Cell proliferation, division, migration, and invasion |
| Breast T47D | Cell proliferation, division, migration, and invasion |
| Colorectal adenocarcinoma DLD-1 cells | Cell proliferation and cell matrix interactions |
| Epithelial ovarian OV-MZ-6 | Promoted spheroid formation and proliferation |
| Epithelial ovarian SKOV-3 | Promoted spheroid formation and proliferation |
| Fuji Cells | Cell proliferation and cell matrix interactions |
| Glioblastoma SF 268 | Cell proliferation and cell matirx interaction |
| Glioblastoma SF 295 | Cell proliferation and cell matirx interaction |
| Glioblastoma SNB75 | Cell proliferation and cell matirx interaction |
| Glioblastoma U-251 MG | Cell proliferation and cell matirx interaction |
| Glioma U87-MG | Increased cell spreading and actin stress fiber assembly |
| Glioma U87-MG | Cell proliferation and cell matirx interaction |
| Glioma U373-MG | Increased cell adhesion duration and migration (on higher stiffness) |
| HEK 293 | Cell proliferation and cell matrix interactions |
| Huaman colon carcinoma HCT-8 | Cell proliferation and cell matirx interaction |
| Human colorectal carcinoma HCT 116 | cell proliferation, cell survival, and intercelluar networking |
| Human pancreatic cancer PANC-1 | cell proliferation and cellular interactions |
| Insulinoma ins-1 (Rat) | Cell proliferation and cell matrix interactions |
| Liver carcinoma HepG2 | Cell proliferation and cell matirx interaction |
| Melanoma Cells | Cell proliferation and cell matrix interactions |
| Ovarian carcinoma OVCAR-3 | Cell proliferation and invasion |
| Primary glioblastom U87 | cell proliferation and cellular interactions |
| Prostate adenocarcinoma LNCaP | Increased cell attachment |
| Prostate CRPC | Cell proliferatin and invasion |
| Prostate DU145 | Cell proliferation and invasion |
| Prostate PC3 | Cell proliferation and invasion |
Cartilage
| Cell Type | Behavior |
|---|---|
| Bovine chondrocytes | Increased cell viability and proliferation |
| Bovine chondrocytes | Promoted cell attachment, viability, and stress fiber formation |
| Human chondrocytes | Promoted cell viability and proliferation |
Connect Tissues
| Cell Type | Behavior |
|---|---|
| Fibroblast NIH3T3 | Promoted cell spreading |
| Fibroblasts NIH3T3 | Increased directional cell migration toward gradient |
| Human dermal fibroblasts | Promoted cell survival and spreading |
| Human dermal fibroblasts | Increased cell adhesion and proliferation |
| Human foreskin fibroblasts | Promoted cell spreading |
Epithelial Cells
| Cell Type | Behavior |
|---|---|
| A549 cells | Cell proliferation and invasion |
| MCF-12A | Cell proliferation and invasion |
| Mouse ovarian follicle cells | Cell proliferation and invasion |
Immune Cells
| Cell Type | Behavior |
|---|---|
| Beta TC3 Cells | Cell proliferation and cellular interactions |
Kidney
| Cell Type | Behavior |
|---|---|
| Human embryonic kidney HEK293 | Promoted spheroid formation |
| Madin-Darby Canine Kidney | Promoted formation of structured epithelial cysts |
Liver
| Cell Type | Behavior |
|---|---|
| Human hepatocytes | Increased number of filopodia and synthesis of albumin |
| Mouse hepatocytes | Promoted cell viability |
Lung
| Cell Type | Behavior |
|---|---|
| Alveolar epithelial A549 | Inhibited cell detachment |
| Alveolar epithelial RLE-6TN | Increased cell attachment and mesenchymal differentiation |
Muscle
| Cell Type | Behavior |
|---|---|
| Mouse skeletal myoblasts | Promoted cell attachment, proliferation, and myofibril formation |
| Myoblasts C2C12 | Promoted cell proliferation and differentiation |
Neural
| Cell Type | Behavior |
|---|---|
| Chick dorsal root ganglion cells | Increased neurite length, neurite outgrowth, and neurite number |
| In vivo lesioned rat cortex | Supported angiogenesis and inhibited glial scars |
| In vivo lesioned rat spinal cord | Supported angiogenesis and axon regeneration |
Stem Cells
| Cell Type | Behavior |
|---|---|
| Human embryonic stem cells | Increased retinal pigmented epithelium and optic vesicle development |
| Human iPSC | Cell proliferation, and cell matrix interactions |
| Human mesenchymal stem cells | Increased cell viability |
| Mouse embryonic stem cells | Promoted endothelial cell differentiation |
| Mouse mesenchymal stem cells | Promoted cell spreading and migration |
| Rat mesenchymal stem cells | Increased cell adhesion and spreading |
| Rat mesenchymal stem cells | Promoted cell attachment and differentiation |
Vascular/Cardiac
| Cell Type | Behavior |
|---|---|
| Human aortic smooth muscle cells | Promoted cell attachment |
| Human umbilical vein endothelial cells | Increased cell adhesion, proliferation, migration, and angiogenesis |
| Human umbilical vein endothelial cells | Increased cell adhesion and proliferation |
| Rat neonatal cardiac | Promoted cell attachment and tissue regeneration and prevented apoptosis |
View All Cell Type Behaviors for All VitroGel Products
| Tissue/Organ type | Cell Type | Relate product | Behavior |
|---|---|---|---|
| Beta cell | BL5 human beta cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Beta TC3 cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cellular interations | |
| Bone | Bone marrow stromal cells (rat) | VitroGel Hydrogel Matrix, VitroGel RGD | Osteogenesic differentiation |
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell viability, and cellular networking | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment and osteoblast differentiation | ||
| Bone marrow mesenchymal stem cells (human) | VitroGel Hydrogel Matrix, VitroGel COL | Chondrogenic/osteogenic differentiation | |
| VitroGel Hydrogel Matrix, VitroGel IKVAV | Angiogenesis | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading, proliferation, and collagen II production | ||
| Bone marrow mesenchymal stem cells (goat) | VitroGel Hydrogel Matrix, VitroGel RGD | Osteogenesic differentiation | |
| Osteoblasts (rat) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment and spreading | |
| Bone marrow stromal cells (bovine) | VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading and osteocalcin expression | |
| Breast | Mammary gland MCF10A | VitroGel Hydrogel Matrix, VitroGel MMP | MMP activity in response to TGF-ß1 |
| Mammary epithelium (mouse) | VitroGel Hydrogel Matrix, VitroGel COL | Cell invasion and dissemination | |
| Cancer/tumor | Human colorectal carcinoma HCT 116 | VitroGel Hydrogel Matrix, VitroGel RGD | cell proliferation, cell survival, and intercelluar networking |
| Huaman colon carcinoma HCT-8 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioma U87-MG | VitroGel Hydrogel Matrix, VitroGel RGD | Cell spreading and actin stress fiber assembly | |
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell migration dependent on mechanical force | ||
| VitroGel Hydrogel Matrix, VitroGel MMP | cell proliferation, spreading, and migration | ||
| Primary glioblastom U87 | VitroGel Hydrogel Matrix, VitroGel RGD | cell proliferation and cellular interations | |
| Glioblastoma SF 268 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioblastoma SF 295 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioblastoma SNB75 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioblastoma U-251 MG | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Prostate PC3 | VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and reduced MMP release | |
| VitroGel Hydrogel Matrix, VitroGel IKVAV | cell proliferation and invasion | ||
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and invasion | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell invasion, migration, and spheroid metabolic activity | ||
| Prostate LNCaP | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and prostate specific antigen release | ||
| Prostate CRPC | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferatin and invasion | |
| Prostate DU145 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and invasion | |
| Melanoma B16F10 | VitroGel Hydrogel Matrix, VitroGel COL | Cell migration, invasion, and MMP release | |
| VitroGel Hydrogel Matrix, VitroGel YIGSR | Cell attachment and spreading | ||
| Breast MDA-MB-231 | VitroGel Hydrogel Matrix, VitroGel MMP | Cell invasion | |
| VitroGel Hydrogel Matrix | Cell spreading | ||
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, division, migration, and invasion | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading and cluster growth | ||
| Fibrosarcoma HT1080 | VitroGel Hydrogel Matrix, VitroGel COL | Cell infiltration | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment | ||
| Breast T47D | VitroGel Hydrogel Matrix, VitroGel COL | Force dependent tubule formation | |
| VitroGel Hydrogel Matrix | Cell cluster growth | ||
| VitroGel Hydrogel Matrix, VitroGel 3D | Spheroid formation and proliferation | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Cell cluster growth | ||
| Breast 4T1 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation | |
| Breast CTC | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Breast E0771 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, spheroid formation | |
| Breast AU-565 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell matrix interations | |
| Epithelial ovarian OV-MZ-6 | VitroGel Hydrogel Matrix, VitroGel RGD | Spheroid formation and proliferation | |
| Epithelial ovarian SKOV-3 | VitroGel Hydrogel Matrix, VitroGel RGD | Spheroid formation and proliferation | |
| Glioma U373-MG | VitroGel Hydrogel Matrix, VitroGel RGD | Cell adhesion and migration | |
| Rhabdomyosarcoma (human) | VitroGel Hydrogel Matrix, VitroGel YIGSR | Cell attachment and spreading | |
| Melanoma SK-MEL-28 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell adhesion and proliferation | |
| Melanoma K-1735 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell invasion | |
| Melanoma A2058 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Collagenolytic activity | |
| Brainstem glioma DIPG | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation and survival | |
| Hela Cells | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Colorectal adenocarcinoma DLD-1 cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Glioma LRM55 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell attachment | |
| Melanoma WM239A | VitroGel Hydrogel Matrix, VitroGel MMP | Cell invasion | |
| Melanoma Cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Insulinoma ins-1 (Rat) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| HEK 293 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Biphasic synovial sarcoma SYO-1 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell matirx interaction, and cell survival | |
| Fuji Cells | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | |
| Chordoma Cells | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Bone OSA 1777 | VitroGel Hydrogel Matrix, VitroGel RGD | spheroid and cluster formation | |
| Glioma RuGli | VitroGel Hydrogel Matrix, VitroGel COL | Integrin dependent cell adhesion | |
| Breast Cancer MCF-7 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, intercellular connections | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation, morphological changes, MMP expression, and angiogenesis | ||
| Liver carcinoma HepG2 | VitroGel Hydrogel Matrix, VitroGel COL | Cell viability, growth, and drug resistance | |
| VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation and cell matirx interaction | ||
| Human pancreatic cancer PANC-1 | VitroGel Hydrogel Matrix, VitroGel RGD | cell proliferation and cellular interations | |
| Primary breast (human) | VitroGel Hydrogel Matrix, VitroGel COL | Cell invasion, migration, and dissemination | |
| Ovarian carcinoma OVCAR-3 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell proliferation, cell matrix interations | |
| Ovarian OVCA429 | VitroGel Hydrogel Matrix, VitroGel COL | MMP dependent cell invasion | |
| Human osteosarcoma KHOS | VitroGel Hydrogel Matrix, VitroGel 3D | cell proliferation and spheroids formation | |
| Human osteosarcoma U2OS | VitroGel Hydrogel Matrix, VitroGel 3D | cell proliferation and spheroids formation | |
| Priess human lymphoblastoid cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| Cartilage | Chondrocytes (bovine) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability and proliferation |
| Chondrocytes (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability and proliferation | |
| Connective tissue | Dermal fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability and spreading |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell viability | ||
| Fibroblasts NIH3T3 | VitroGel Hydrogel Matrix, VitroGel RGD | Directional cell migration toward gradient | |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading dependent on substrata rigidity | ||
| Foreskin fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell spreading | |
| VitroGel Hydrogel Matrix, VitroGel YIGSR | Cell spreading | ||
| VitroGel Hydrogel Matrix, VitroGel MMP | Substrata degradation and cell invasion | ||
| Skin fibroblasts (skin) | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell adhesion | |
| Epidermal keratinocytes | VitroGel Hydrogel Matrix, VitroGel COL | Cell viability | |
| Epithelial Cells | Mouse ovarian follicle cells | VitroGel Hydrogel Matrix, VitroGel RGD | 3D cell culture using ES-hydrogel can enhance vitro follicle culture by considering the permeability and stiffness of the gel. |
| Human Nthy-ori 3-1 cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| A549 cells | VitroGel Hydrogel Matrix, VitroGel RGD | Enhance cell proliferation and cell matrix interactions. | |
| MCF-12A | VitroGel Hydrogel Matrix, VitroGel RGD | Enhance cell proliferation and cell matrix interactions. | |
| Immortalized bronchial epithelial cells HBEC-KRAS | VitroGel Hydrogel Matrix, VitroGel 3D | Cell proliferation | |
| Eye | Corneal endothelial B4G12 | VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment and spreading |
| Retinal ganglion cells (xenopus) | VitroGel Hydrogel Matrix, VitroGel COL | Neurite outgrowth | |
| Immune Cells | CD8 + T cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Kidney | Human embryonic kidney HEK293 | VitroGel Hydrogel Matrix, VitroGel RGD | 3D spheroids formation |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and cluster growth | ||
| Madin-Darby Canine Kidney | VitroGel Hydrogel Matrix, VitroGel RGD | Epithelial cysts formation | |
| podocytes (human) | VitroGel Hydrogel Matrix, VitroGel COL | Glomerular capillary formation | |
| glomerular endothelial cells (human) | VitroGel Hydrogel Matrix, VitroGel COL | Glomerular capillary formation | |
| Liver | Hepatocytes (human) | VitroGel Hydrogel Matrix, VitroGel RGD | Filopodia formation and synthesis of albumin |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment | ||
| Hepatocytes (mouse) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell viability | |
| Hepatocytes (rat) | VitroGel Hydrogel Matrix, VitroGel COL | Albumin secretion | |
| Hepatocytes (swine) | VitroGel Hydrogel Matrix, VitroGel COL | Cell spreading and albumin section | |
| Lung | Alveolar basal epithelial A549 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment |
| Alveolar epithelial RLE-6TN | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment and mesenchymal differentiation | |
| Pulmonary fibroblasts LL2 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Cell adhesion | |
| HFL1 lung fibroblasts CCL153 | VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation and spindle morphology | |
| Lung cancer associated fibroblasts (human) | VitroGel Hydrogel Matrix, VitroGel COL | Substrata contractility | |
| Lung fibroblasts MCR-5 | VitroGel Hydrogel Matrix, VitroGel COL | NGF-mediated substrata contraction | |
| Muscle | Myoblasts C2C12 | VitroGel Hydrogel Matrix, VitroGel RGD | Cell Proliferation and differentiation |
| VitroGel Hydrogel Matrix, VitroGel COL | Cell attachment, proliferation, and myofibril formation | ||
| VitroGel Hydrogel Matrix | Myotube formation | ||
| VitroGel Hydrogel Matrix, VitroGel COL | Integrin dependent cell adhesion | ||
| Skeletal myoblasts (mouse) | VitroGel Hydrogel Matrix, VitroGel RGD | Cell attachment, proliferation, and myofibril formation | |
| Myoblasts (human) | VitroGel Hydrogel Matrix, VitroGel COL | Cell adhesion, alignment along fiber, and myotube formation | |
| Myoblasts C25Cl48 | VitroGel Hydrogel Matrix, VitroGel COL | Cell proliferation, differentiation and myotube formation | |
| Neural | Dorsal root ganglion (chick) | VitroGel RGD | Neurite formation and outgrowth |
| VitroGel COL | Force dependent neurite outgrowth | ||
| Neural PC12 | VitroGel COL | Neurite outgrowth | |
| VitroGel IKVAV | Neurite outgrowth | ||
| Neural stem cell/progenitor cell (rat) | VitroGel YIGSR | Cell viability | |
| VitroGel IKVAV | Cell attachment and differentiation | ||
| Neural stem cell/progenitor cell (human) | VitroGel IKVAV | Cell viability and differentiation | |
| VitroGel LDP1 | Cell viability and differentiation | ||
| VitroGel LDP1 | Cell viability | ||
| VitroGel COL | Cell attachment | ||
| Schwann cells (rat) | VitroGel YIGSR | Cell attachment and migration | |
| Neural stem cell/progenitor cell (mouse) | VitroGel IKVAV | Cell adhesion and differentiation | |
| Cortical astrocytes (rat) | VitroGel IKVAV | Cell adhesion | |
| Spiral ganglion neurons (mouse) | VitroGel IKVAV | Neurite outgrowth | |
| Motor neurons (human) | VitroGel COL | Force dependent neurite outgrowth | |
| Forebrain neurons (human) | VitroGel COL | Force dependent neurite outgrowth | |
| Cortical neurons (rat) | VitroGel COL | Neuronal viability and neurite outgrowth | |
| Dorsal root ganglion (rat) | VitroGel COL | Neurite outgrowth | |
| Red Blood Cells | Red Blood cells | VitroGel Hydrogel Matrix, VitroGel 3D | Enhance spheroids and cluster formation and promote cell viability. |
| Pancreas | B-cells MIN6 | VitroGel Hydrogel Matrix, VitroGel IKVAV | Reduced apoptosis and increased insulin release |
| Stem cells | Mesenchymal stem cells (human) | VitroGel RGD | Cell viability |
| VitroGel RGD | Cell Proliferation and differentiation | ||
| VitroGel COL | Cell proliferation | ||
| VitroGel IKVAV | Neuronal differentiation | ||
| VitroGel MMP | Neuronal differentiation and neurite outgrowth | ||
| VitroGel COL | Cell attachment, spreading, viability, and osteoblast differentiation | ||
| Mesenchymal stem cells (mouse) | VitroGel RGD | Cell spreading and migration | |
| VitroGel MMP | Cell spreading and migration | ||
| Mesenchymal stem cells (rat) | VitroGel RGD | Cell adhesion and spreading | |
| Embryonic stem cells (mouse) | VitroGel RGD | Endothelial cell differentiation | |
| VitroGel COL | Neuronal differentiation and neurite outgrowth | ||
| VitroGel YIGSR | Neuronal differentiation | ||
| Induced pluripotent stem cells (human) | VitroGel YIGSR | Cell viability | |
| VitroGel IKVAV | Cell viability | ||
| VitroGel LDP1 | Cell viability | ||
| Human Ipsc | VitroGel RGD | Cell proliferation, and cell matrix interactions | |
| Human stem cells from apical papilla SCAP | VitroGel 3D | Cell viability | |
| Adipose derived stem cells (human) | VitroGel IKVAV | Cell attachment | |
| Vascular/cardiac | Umbilical vein endothelial cells (human) | VitroGel RGD | Cell attachment, proliferation, migration, and angiogenesis |
| VitroGel YIGSR | Upregulation in gene expression | ||
| VitroGel IKVAV | Migratory cell infiltration | ||
| VitroGel MMP | Cell attachment, migration, and survival | ||
| VitroGel COL | Cell attachment, spreading, and VEGF dependent migration | ||
| Neonatal cardiac (rat) | VitroGel RGD | Cell attachment and tissue regeneration | |
| VitroGel YIGSR | Cell attachment similar to laminin | ||
| Aortic smooth muscle cells (human) | VitroGel RGD | Cell attachment | |
| Endothelial (human) | VitroGel YIGSR | Cell differentiation | |
| Endotheliocytes | VitroGel YIGSR | Cell migration | |
| Microvascular endothelial cells (human) | VitroGel YIGSR | Cell mobility | |
| Aortic endothelial cells (bovine) | VitroGel COL | Force dependent cell spreading | |
| Capillary endothelial cells (bovine) | VitroGel COL | Capillary like network formation |
Data
Figure 1. Rheological properties of VitroGel RGD with DMEM medium.
A) – C) The gel formation curve after mixing with DMEM (A), DMEM/F-12 (B), and RPMI (C) media. VitroGel RGD was diluted at 1:0,1:1, 1:2 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with media at 4:1 (v/v) ratio; D) – F) The gel strength after 24 hrs incubation in DMEM (D), DMEM/F-12 (E), and RPMI (F) media. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa)
Figure 2. 3D culture of OP9 cells in VitroGel RGD.
Hydrogel was prepared at 1:3 dilution with VitroGel Dilution Solution (Type 1). The images were taken on days 2 and 7. VitroGel RGD shows support for OP9 cell proliferation and cell-cell communication. The stronger cell-matrix interactions help the cells to form the cell-networking structure.
Figure 3. 3D view of OP9 cells growth in VitroGel RGD.
Cell networking structure formed in VitroGel RGD.
Figure 4. 3D culture of U87-MG cells in VitroGel RGD.
Cells can grow in 3D hydrogel at 1:1 and 1:3 dilution of VitroGel RGD. U87-MG cells shows cell networking structure and the cell morphology in VitroGel RGD indicating a cell-cell and cell-matrix interaction.
References/Publications
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| Size | 3 mL Kit |
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