Room Temperature Operation
Room temperature protocol/operation. No ice bucket required.
VitroGel® COL High Concentration
collagen-mimetic functional hydrogel – tunable, xeno-free (3 mL)
VitroGel® COL High Concentration
Tunable, xeno-free, collagen-mimetic hydrogel for promoting osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo in tissue engineering applications.
Xeno-free
100% synthetic. Animal & human origin-free, biofunctional hydrogel.
Tunable Hydrogel Strength
Adjust the hydrogel strength from 10 Pa to over 20,000 Pa to create the optimal cell environment.
Mix & Match
Build and create a customized multifunctional hydrogel by blending different types of VitroGel® together.
Easy-to-use No cross-linking agent required. Adjust hydrogel with Dilution Solution, mix with cells, add medium and incubate.
Easy cell harvesting Simple and efficient cell harvesting by the non-enzymatic VitroGel® Organoid Recovery Solution.
VitroGel® COL High Concentration
VitroGel® COL High Concentration is a tunable, xeno-free hydrogel system that mimics the functions of native collagen. The hydrogel can promote osteoblastic differentiation in vitro and enhance osteoblastic activity in vivo, which shows great potential for tissue engineering and regenerative medicine applications. VitroGel® COL High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, is transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture to 2D cell coating and animal injection, VitroGel® makes it possible to bridge in vitro and in vivo studies with the same platform system.
Mix & Match – 3D Cell Culture Your WAY!
Unique to VitroGel® High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel® COL can be “mix & matched” with other versions of VitroGel®, such as VitroGel® RGD, VitroGel® IKVAV, VitroGel® YIGSR, and VitroGel® MMP to create a customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists can customize their 3D culture micro-environment for different applications.
Specifications
| Contents | VitroGel® COL High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
| Hydrogel Formulation | Xeno-free tunable collagen-mimetic functional hydrogel. |
| Use | Support integrin binding to promote osteoblastic differentiation in vitro and enhancing osteoblastic activity in vivo |
| Mix & Match | Can be blended with other versions of VitroGel concentrated hydrogels to create a custom multi-functional matrix. |
| Operation | Room temperature |
| Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
| pH | Neutral |
| Color | Transparent |
| Cell Harvesting | VitroGel Organoid Recovery Solution 5-15 min cell recovery |
| Injectable | Injectable hydrogel |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Number of Uses | Dilution ratio: 1:2 = 225 uses at 50 µL per well 1:3 = 300 uses at 50 µL per well 1:5 = 450 uses at 50 µL per well |
3D Cell Culture Process in 20 Minutes
VitroGel® High Concentration hydrogels are easy to use. There is no cross-linking agent required. Work confidently at room temperature.
Tunable Hydrogel Strength
Simply diluting the hydrogel controls the gel strength.
Video Protocols & Demonstrations
Data and References
Cell Type Behavior Reference Table – VitroGel® COL
Studies have been performed using VitroGel® COL in different tissues and cell types.
Bone
| Cell Type | Behavior |
|---|---|
| Bovine bone marrow stromal cells | Increased cell spreading and osteocalcin expression |
| Human bone | Increased cell spreading, proliferation, and collagen II production |
| marrow mesenchymal stem cells | |
| Rat bone marrow stromal cells | Increased cell adhesion and osteoblast differentiation |
| Human bone marrow-derived mesenchymal stem cells | Promoted calcium deposition and chondrogenic/ |
| osteogenic differentiation | |
| Mouse bone marrow stromal cells | Supports osteogenesis |
Breast
| Cell Type | Behavior |
|---|---|
| Mouse mammary epithelium | Promoted transient cell invasion and dissemination |
Cancer/Tumor
| Cell Type | Behavior |
|---|---|
| Breast MDA-MB-231 | Increased cell cluster size and spreading |
| Breast T47D | Increased cell cluster size |
| Breast T47D | Promoted force dependent tubule formation |
| Breast MCF-7 | Increased cell proliferation, morphological changes, MMP expression, and angiogenesis |
| Co-culture of liver carcinoma HepG2 and stromal fibroblasts 3T3-J2 | Increased cell viability, growth, and drug resistance |
| Fibrosarcoma HT1080 | Support cell infiltration and growth |
| Fibrosarcoma HT1080 | Promoted integrin dependent cell adhesion |
| Fibrosarcoma HT1080 | Promoted cell adhesion |
| Glioma RuGli | Promoted integrin dependent cell adhesion |
| Glioma U87-MG | Cell migration dependent on mechanical force |
| Prostate PC3 | Increased cell invasion, migration, and spheroid metabolic activity |
| Human primary breast | Promoted cell invasion, migration, and dissemination |
| Melanoma B16F10 | Increased cell migration, invasion, and MMP release |
| Ovarian OVCA429 | MMP dependent cell invasion |
| Prostate LNCaP | Supported cell proliferation and increased prostate-specific antigen release |
| Prostate PC3 | Supported cell proliferation and reduced MMP release |
Connect Tissues
| Cell Type | Behavior |
|---|---|
| Co-culture human dermal fibroblasts and epidermal keratinocytes | Promoted cell viability |
| Fibroblast NIH3T3 | Increased cell spreading on rigid |
Eye
| Cell Type | Behavior |
|---|---|
| Corneal endothelial B4G12 | Increased cell attachment and spreading |
| Xenopus retinal ganglion cells | Promoted neurite outgrowth |
Liver
| Cell Type | Behavior |
|---|---|
| Human Hep3B | Promoted cell attachment |
| Rat hepatocytes | Promote albumin secretion |
| Swine hepatocytes | Promoted cell spreading and albumin section |
Lung
| Cell Type | Behavior |
|---|---|
| Lung fibroblasts HFL1 (CCL153) | Promoted cell proliferation and spindle morphology |
| Human lung cancer associated fibroblasts | Increased smooth muscle actin and substrata contractility |
| Lung fibroblasts MCR-5 | Promoted NGF-mediated substrata contraction |
Muscle
| Cell Type | Behavior |
|---|---|
| Human myoblasts | Promoted cell adhesion, alignment along fiber, and myotube formation |
| Mouse myoblast C2C12 | Promote integrin dependent cell adhesion |
| Myoblasts C2C12 | Promoted formation of myotubes and myotendinous |
| Myoblasts C2C12 | Promote cell proliferation, differentiation, and myotube formation |
| Myoblasts C25Cl48 | Promote cell proliferation, differentiation, and myotube formation |
Neural
| Cell Type | Behavior |
|---|---|
| Human neural stem/progenitor cells | Promoted cell attachment |
| Chick dorsal root ganglion | *Increased neurite length on soft |
| Chick dorsal root ganglion | *Increased neurite outgrowth toward soft |
| Human motor neurons | *Increased neurite length on rigid |
| Human forebrain neurons | *Increased neurite length on soft |
| Neural PC12 | Increased neurite length |
| Rat cortical neurons | Increased neuronal viability and neurite length |
| Rat dorsal root ganglion | Promoted axon outgrowth |
| Rat dorsal root ganglion | Promoted neurite outgrowth |
| Rat spinal cord section | Promoted neurite outgrowth |
Stem Cells
| Cell Type | Behavior |
|---|---|
| Human mesenchymal stem cells | Promoted cell adhesion, spreading, viability, and osteoblast differentiation |
| Human mesenchymal stem cells | Promoted chondrogenic differentiation |
| Human mesenchymal stem cells | Increased cell migration, proliferation, and osteogenic differentiation |
| Human mesenchymal stem cells | Promoted cell attachment and tenogenic differentiation |
| Human mesenchymal stem cells | Promoted cell proliferation |
| Mouse embryonic stem cells | Supported neuronal differentiation and neurite outgrowth |
Vascular
| Cell Type | Behavior |
|---|---|
| Bovine aortic endothelial cells | Promoted cell spreading along fiber |
| Bovine aortic endothelial cells | Increased cell spreading on rigid |
| Bovine capillary endothelial cells | Formation of capillary like networks |
| Human umbilical vein endothelial cells | Increased VEGF dependent vascularization |
| Human umbilical vein endothelial cells | Promoted cell adhesion, spreading and supported increased VEGF dependent migration |
View All Cell Type Behaviors for All VitroGel® Products
| TISSUE/ORGAN TYPE | CELL TYPE | READY TO USE | HIGH CONCENTRATION | BEHAVIOR | |
|---|---|---|---|---|---|
| Beta Cell | BL5 human beta cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D VitroGel® MMP | Enhance spheroids formation | |
| Beta TC3 cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cellular interactions | ||
| Bone | Bone marrow stromal cells (rat) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Osteogensic differentiation Cell attachment and osteoblast differentiation Cell proliferation cell viability and cellular networking | |
| Osteoblasts (rat) | VitroGel® MSC VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell attachment and spreading | ||
| Bone marrow stromal cells (bovine) | VitroGel® MSC VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell spreading and osteocalcin expression | ||
| Breast | Mammary gland MCF10A | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitrolGel RGD VitroGel® COL VitroGel® MMP | Spheroid formation MMP activity in response to TGF-B1 | |
| Mammary epithelium (mouse) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell invasion and dissemination | ||
| Cancer/Tumor | Human colorectal carcinoma HCT 116 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation cell survival and intercellular networking | |
| Huaman colon carcinoma HCT-8 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Glioma U87-MG | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP VitroGel® COL | Cell spreading and acting stress fiber assembly cell proliferation spreading and migration Cell migration dependent on mechancial force Cell proliferation and cell matrix interaction | ||
| Gliobastoma SF 268 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitrolGel RGD | Cell proliferation and cell matrix interaction | ||
| Gliobastoma SF 295 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Glioblastoma SNB75 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Glioblastoma U-251 MG | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Prostate PC3 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® COL VitroGel® IKVAV VitroGel® RGD VitroGel® MMP | Cell proliferation reduced MMP release invasion migration and spheroid metabolic activity. | ||
| Prostate LNCaP | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell attachment proliferation and prostate specific antigen release | ||
| Prostate CRPC | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and invasion | ||
| Prostate DU145 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and invasion | ||
| Melanoma B16F10 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® COL VitroGel® YIGSR | Cell migration invasion MMP release cell attachment and spreading | ||
| Breast MDA-MB-231 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP VitroGel® 3D | Cell invasion spreading proliferation division migration and cluster growth | ||
| Fibrosarcoma HT1080 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell infiltration attachment | ||
| Breast T47D | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® COL VitroGel® 3D VitroGel® RGD VitroGel® MMP | Force dependent tubule formation cell cluster growth spheroid formation and proliferation | ||
| Breast 4T1 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation | ||
| Breast CTC | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D VitroGel® RGD | Cell proliferation | ||
| Breast E0771 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation spheroid formation | ||
| Brest AU-565 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation cell matrix interactions | ||
| Epithelial ovarian OV-MZ-6 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Spheroid formation and proliferation | ||
| Epithelial ovarian SKOV-3 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Spheroid formation and proliferation | ||
| Glioma U373-MG | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell adhesion invasion and migration | ||
| Rhabdomyosarcoma (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® YIGSR | Cell attachment and spreading | ||
| Melanoma SK-MEL-28 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Cell adhesion and proliferation | ||
| Melanoma K-1735 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Cell invasion | ||
| Melanoma A2058 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Collagenolytic activity | ||
| Brainstem glioma DIPG | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell proliferation and survival | ||
| Hela Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D VitroGel® RGD VitroGel® MMP | Cell proliferation | ||
| Colorectal adenocarcinoma DLD-1 cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Giloma LRM55 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® IKVAV VitroGel® MMP | Cell attachment | ||
| Melanoma WM 239A | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell invasion | ||
| Melanoma Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Insulinoma ins-1 (Rat) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Biphasic synovial sarcoma SYO-1 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation cell matrix interation and cell survival | ||
| Fuji Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Chordoma Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Cell proliferation | ||
| Bone OSA 1777 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Spheroid and cluster formation | ||
| Glioma RuGli | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® COL | Integrin dependent cell adhesion | ||
| Breast Cancer MCF-7 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP VitroGel® 3D | Cell proliferation intercellular connections morphological changes MMP expression and angiogenesis | ||
| Liver carcinoma HepG2 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell viability growth drug resistance proliferation and cellular matrix interaction | ||
| Human pancreatic cancer PANC-1 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell proliferation and cellular interactions | ||
| Primary breast (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell invasion migration and dissemination | ||
| Ovarian carcinoma OVCAR-3 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP | Cell proliferation cell matrix interactions | ||
| Ovarian OVCA429 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP VitroGel® COL | MMP dependent cell invasion | ||
| Human Osteosarcoma KHOS | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® 3D | Cell proliferation and spheroids formation | ||
| Human Osteosarcoma U2OS | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® 3D | Cell proliferation and spheroids formation | ||
| Human fibroblast-like synoviocytes (FLS) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Cell proliferation and inflammatory responses | ||
| Human Liposarcoma 94T778 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Cell proliferation and spheroids formation | ||
| Human diffuse large B-cell lymphoma (DLBLC) SUDHL-10 | VitroGel® Hydrogel Matrix | Cell viability growth drug resistance proliferation and cellular matrix interaction | |||
| Priess human lymphoblastoid cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Enhance spheroids and cluster formation and promote cell viability. | ||
| Cartilage | Chondrocytes (bovine) | VitroGel® MSC VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell viability and proliferation | |
| Chondrocytes (human) | VitroGel® MSC VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell viability and proliferation | ||
| Connective Tissue | Dermal Fibroblasts (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell viability and spreading | |
| Fibroblasts NIH3T3 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Directional cell migration toward gradient and cell spreading dependent on substrata rigidity | ||
| Foreskin fibroblasts (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® YIGSR VitroGel® MMP | Cell spreading substrata degradation and cell invasion | ||
| Skin fibroblasts (skin) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® IKVAV | Cell adhesion | ||
| Epidermal keratinocytes | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell viability | ||
| Epithelial Cells | Mouse ovarian follicle cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | 3D cell culture using ES-hydrogel can enhance vitro follicle culture by considering the permeability and stiffness of the gel. | |
| Human Nthy-ori 3-1 cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Enhance spheroids and cluster formation and promote cell viability. | ||
| A549 cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Enhance cell proliferation and cell matrix interactions. | ||
| MCF-12A | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Enhance cell proliferation and cell matrix interactions. | ||
| Immortalized bronchial epithelial cells HBEC-KRAS | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® 3D | Cell proliferation | ||
| Eye | Corneal endothelial B4G12 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit VitroGel® Angiogenesis Assay | VitroGel® RGD VitroGel® Angiogenesis Assay HC kit | Cell attachment and spreading | |
| Retinal ganglion cells (xenopus) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Neurite outgrowth | ||
| Immune Cells | CD8 + T cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| Kidney | Human embryonic kidney HEK293 | VitroGel® Hydrogel Matrix VitroGel® HEK293 VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | 3D spheroids formation | |
| Madin-Darby Canine Kidney | VitroGel® Hydrogel Matrix VitroGel® HEK293 VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP | Epithelial cysts formation | ||
| Podocytes (human) | VitroGel® Hydrogel Matrix VitroGel® HEK293 VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Glomerular capillary formation | ||
| glomerular endothelial cells (human) | VitroGel® Hydrogel Matrix VitroGel® HEK293 VitroGel® ORGANOID Disovery Kit VitroGel® Angiogenesis Assay | VitroGel® RGD VitroGel® Angiogenesis Assay HC kit | Glomerular capillary formation | ||
| Liver | Hepatocytes (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Filopodia formation and synthesis of albumin and cell attachment | |
| Hepatocytes (mouse rat swine) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell viability spearding Albumin secretion | ||
| Lung | Alveolar basal epithelial A549 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell attachment | |
| Alveolar epithelial RLE-6TN | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell attachment and mesenchymal differentiation | ||
| Pulmonary fibroblasts LL2 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® IKVAV | Cell adhesion | ||
| HFL1 lung fibroblasts CCL153 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and spindle morphology | ||
| Lung cancer associated fibroblasts (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Substrata contractility | ||
| Lung fibroblasts MCR-5 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | NGF-mediated substrata contraction | ||
| Muscle | Myoblasts C2C12 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell proliferation differentiation attachment myofibril formation myotube formation and integrin dependent cell adhesion | |
| Skeletal myoblasts (mouse) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell attachment proliferation and myofibril formation | ||
| Myoblasts (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell adhesion alignment along fiber and myotube formation | ||
| Myoblasts C25Cl48 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell proliferation differentiation and myotube formation | ||
| Neural | Dorsal root ganglion (chick) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit VitroGel® NEURON | VitroGel® RGD VitroGel® COL | Neurite formation and force dependent neurite outgrowth | |
| Neural PC12 | VitroGel® Hydrogel Matrix | VitroGel® RGD VitroGel® IKVAV | Neurite outgrowth | ||
| Neural stem cell/ progenitor cell (rat) | VitroGel® STEM VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV | Cell viability attachment and differentiation | ||
| Neural stem cell/ progenitor cell (human) | VitroGel® STEM VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV VitroGel® COL | Cell viability attachment and differentiation | ||
| Schwann cells (rat) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD | Cell attachment and migration | ||
| Neural stem cell/ progenitor cell (mouse) | VitroGel® STEM VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV | Cell adhesion and differentiation | ||
| Cortical astrocytes (rat) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV | Cell adhesion | ||
| Spiral ganglion neurons (mouse) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV | Neurite outgrowth | ||
| Motor neurons (human) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Force dependent neurite outgrowth | ||
| Forebrain neurons (human) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Force dependent neurite outgrowth | ||
| Cortical neurons (rat) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Neuronal viability and neurite outgrowth | ||
| Dorsal root ganglion (rat) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Neurite outgrowth | ||
| Red Blood Cells | Red Blood Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Enhance Spheroids and cluster formation and promote cell viability | |
| Pancreas | B-cells MIN6 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® IKVAV | Reduced apoptosis and increased insulin release | |
| Stem Cells | Mesenchymal stem cells (human) | VitroGel® MSC | VitroGel® RGD VitroGel® COL VitroGel® IKVAV VitroGel® MMP | Cell viability proliferation differentiation neuronal differntiation neurite outgrowth attachment spreading viability and osteoblast differentiation | |
| Mesenchymal stem cells (mouse) | VitroGel® MSC | VitroGel® RGD VitroGel® MMP | Cell spreading and migration | ||
| Mesenchymal stem cells (rat) | VitroGel® MSC | VitroGel® RGD | Cell adhesion and spreading | ||
| Embryonic stem cells (mouse) | VitroGel® STEM VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® YIGSR | Endothelial cell differentiation neuronal differentiation and neurite outgrowth | ||
| Induced pluripotent stem cells (human) | VitroGel® STEM VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® YIGSR VitroGel® IKVAV | Cell viability | ||
| Human stem cells from apical papilla SCAP | VitroGel® STEM | VitroGel® RGD | Cell viability | ||
| Hematopoietic Stem Cells | VitroGel® STEM | Cell viability | |||
| Adipose derived stem cells (human) | VitroGel® MSC | VitroGel® RGD VitroGel® 3D VitroGel® IKVAV | Cell viability cell attachment | ||
| Vascular/cardiac | Umbilical vein endothelial cells | VitroGel® Angiogenesis Assay VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® Angiogenesis Assay HC kit | Cell attachment proliferation migration angiogenesis gene expression changes migratory cell infiltration cell survival and VEGF dependent migration | |
| Neonatal cardiac (rat) | VitroGel® Angiogenesis Assay VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® Angiogenesis Assay HC kit | Cell attachment tissue regeneration and attachment similar to laminin | ||
| Aortic smooth muscle cells | VitroGel® Hydrogel Matrix | VitroGel® Angiogenesis Assay HC kit | Cell attachment | ||
| Endothelial (human) | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Cell differentiation | ||
| Endotheliocytes | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Cell migration | ||
| Microvascular endothelial cells (human) | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Cell mobility | ||
| Aortic endothelial cells (bovine) | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Force dependent cell spreading | ||
| Capillary endothelial cells (bovine) | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Capillary like network formation |
Data
Figure 1. Rheological properties of VitroGel® COL with DMEM medium.
A) The gel formation curve after mixing with DMEM medium. VitroGel® COL was diluted at 1:0,1:1, and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mixed with DMEM at a 4:1 (v/v) ratio. B) The gel strength after 24 hours of incubation. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test.
Figure 2. 3D culture of mouse bone marrow stromal cells (OP9 mesenchyme) in the mixture of VitroGel® COL.
Cells were cultured with 1:5 diluted VitroGel® COL The single cells were suspended in the hydrogel matrix (Day 1) and extended to form a fibroblast-like cell-matrix structure (Day 7).
Figure 3. 3D culture of glioblastoma cells (SNB-75) in VitroGel® COL.
Cells were cultured with 1:5 diluted VitroGel® COL, following the user handbook (50% FBS was used to prepare the cell suspension to get a hydrogel with a final 10% FBS concentration).
Publications
- Acimovic, I., Chochola, V., Herrera, J. L., Hampl, A., & Jaros, J. (2025). 3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors. Scientific Reports, 15(1). https://doi.org/10.1038/s41598-025-25381-x
- Simões, B. M., Pedley, R., McCloskey, C. W., Roberts, M., Reed, A. D., Twigger, A.-J., Pirashaanthy Tharmapalan, Caruso, A., Cabral, S., Wilby, A. J., Harrison, H., Zhou, Y., Greenhalgh, A., Alghamdi, S. A., Forestiero, M., Lopez-Muñoz, J., Roche, J., Ren Jie Tuieng, Khan, M. A., & Squires, S. (2025). Anti-progestin therapy targets hallmarks of breast cancer risk. Nature. https://doi.org/10.1038/s41586-025-09684-7
| High Concentration Kit Type | VitroGel COL + Dilution Solution TYPE 1, VitroGel COL + Dilution Solution TYPE 2 |
|---|
Related products
Hydrogels - Tunable
Matrix metalloproteinases (MMP) sensitive biodegradable hydrogel - tunable, xeno-free hydrogel, high concentration (3 mL kit) Supports biological activities such as cell proliferation, migration (adhesion/dispersion), differentiation, angiogenesis, apoptosis, etc.
Hydrogels - Tunable
RGD modified - tunable, xeno-free hydrogel - high concentration (3 mL kit)
Hydrogels - Tunable
tunable, xeno-free hydrogel, high concentration (3 mL kit)
Hydrogels - Tunable
Laminin-derived functional peptide (IKVAV) modified – tunable, xeno-free hydrogel, high concentration ( 3 mL kit)
Hydrogels - Tunable
Laminin-derived functional peptide (YIGSR) modified – tunable, xeno-free hydrogel, high concentration (3 mL kit)
