In Vivo Applications

Xeno-free VitroGel® Hydrogel
for Tissue Engineering

VitroGel xeno-free (animal-free) hydrogels are excellent for injection and a superior alternative to the animal-based extracellular matrix (ECM) for patient-derived or cell-derived xenograft (PDX & CDX) applications. By avoiding the uncertainty of unknown components from the animal-based ECM, VitroGel hydrogels give well-defined and full control of the microenvironment for consistent results.


How to select the functional hydrogel for your research project

Our xeno-free functional hydrogel system is designed based on the application needs (ready-to-use VitroGel) and the biochemical/biophysical properties needs (high concentration VitroGel). Scientists can choose the hydrogels that fit their research objectives.

All VitroGel hydrogels are injectable and excellent for tissue engineering. Researchers have full control of the supplement/growth factors in the hydrogel-cell mixture.  Below are some hydrogel recommendations based on the popular applications:

> Ready-To-Use VitroGel

The ready-to-use VitroGel hydrogels have optimized formulations of multi-functional ligands and concentrations. Each hydrogel is ready to mix directly with cells/compound suspension, offering an excellent balance of simplicity and versatility. (Click the image on the right to view all different types of ready-to-use VitroGel)

View Injection Protocol

> High Concentration VitroGel

The high concentration hydrogels allow the flexibility to manipulate the mechanical strength of the hydrogel (tunable) by adjusting the hydrogel concentration with the VitroGel Dilution Solution. Each high-concentration hydrogel comes with a functional ligand modification allowing scientists to investigate the cell behaviors with complete control of hydrogel properties. (Click the image on the right to check all different types of high-concentration VitroGel)

  • VitroGel® RGD: One of the most popular hydrogels for multiple in vivo applications. Supports a wide range of cell types with excellent cell-matrix interactions. Great for multiple cell therapy applications. (TWG003)
  • VitroGel® MMP: Great hydrogel degradability to support rapid cell expansion, and invasion/migration. Excellent for tumor study and xenograft. (TWG010)
  • VitroGel® 3D: Pure hydrogel matrix without binding ligand modification. Great for simple target delivery and control release. (TWG001)
View Injection Protocol

Case Studies/Research Highlights

Getting to the root of the problem: Injectable VitroGel® 3D hydrogel scaffolds enhance odontogenic differentiation in vivo

The biocompatible and injectable VitroGel hydrogel culture system enhances pulp-dentin regeneration and has the potential for chair-side regenerative endodontic procedures


ability to enhance the ECM-cell interaction enhances the transplantable capacity of MSCs in rat models of intervertebral disc regeneration

VitroGel® RGD enhances stem cell transplantation to treat intervertebral disc degeneration using rat models, laying new foundations in the movement toward cell-based tissue therapies.


Inhibition of Scar Proliferation with In-situ Cross Bonding of VitroGel Hydrogel and Adipose-derived Stem Cells Conditioned Medium

Developing ADSCC-CM and VitroGel hydrogel complex to study hypertrophic scar prophylaxis.


VitroGel Hydrogel Used to Bridge the Gap Between In Vivo and In Vitro Pancreatic Islet Beta-cells Studies

Scientists used VitroGel RGD to dramatically increase the survival rate and insulin secretory capacity of pancreatic islet beta-cells both in vitro and in vivo. The study provides a reference for using a xeno-free functional hydrogel system for islet transplantation.


The injectable VitroGel provides a viable in vivo scaffold for novel maternal-fetal adipose stem cell transplant studies

Maternally derived adipose stem cells have emerged as a possible solution to the common immunological rejection observed in fetal repair surgeries.


Glucose Metabolism in Obese Mice can be Improved by Implantation with Photoactivated Adipose Stem Cells

VitroGel was used as an injectable adjuvant for in vivo implantation of adipose stem cells to ameliorate glucose intolerance in obese mice.


Research publications Using VitroGel for in vivo applications