VitroPrime™ Ultra-Low Attachment Plate, U-Bottom, 96-Well

Premium U-bottom cell culture plate for 3D spheroids.

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VitroPrime™ Ultra-Low Attachment Plates, 96-well, U-Bottom

The VitroPrime Ultra-Low Attachment, U-bottom plate features a unique surface treatment that prevents cell adhesion, enabling spheroid formation and the creation of advanced 3D models, such as organoids and tumoroids. Designed with a uniform surface coating across wells, the VitroPrime™ Ultra-low Attachment plate ensures experimental consistency that enables drug screening and high-throughput applications. ​

Combined with the VitroGel® hydrogel system, researchers can examine spheroid invasion applications and complex tumoroid models.

Specifications

MaterialPolystyrene (GPPS)
UseSpheroid formation and cultures, organoid and tumoroid cultures
Well Plate Type96-well
Well ProfileU-Bottom
Surface TypeUltra-low adsorption
SterileYes
Other DataDNase/RNase-free, non-pyrogenic

Protocols and Resources

Product Documentation


Sales Sheet – VitroPrime™ Ultra-Low Attachment Plate

Product Data Sheet

Frequently Asked Questions 

Data and References

Spheroid Invasion Assay

Spheroid Formation; No hydrogel (Day 0).

Figure 1. Spheroid invasion assay using the VitroPrime™ Ultra-low Attachment Plate and VitroGel® Hydrogel Matrix
U87-MG glioblastoma cells were resuspended in basal medium with 10% fetal bovine serum. Twenty microliters (20 µL) of cell suspension were added to the VitroPrime™ Ultra-low Attachment Plate, U-bottom, 96-well plate. The cultures were incubated overnight at 37°C to allow spheroid formation. VitroGel® Hydrogel Matrix was combined with serum, and 40 µL of the mixture was added to the spheroid, followed by a 15-minute incubation at room temperature. The images were obtained with the Zeiss Microscope at a 2.5X magnification.

EMT Tumoroid

Figure 2. EMT GBM Tumoroid Viability
U87-MG GBM cells (1 x 10^6 cells/mL) were resuspended in basal medium with supplement system. Twenty microliters (20 µL) of cell suspension were added to the VitroPrime™ Ultra-low Attachment Plate, U-bottom, 96-well plate. The cultures were incubated overnight at 37 °C for spheroid formation. The hydrogel (40 µL) was added to the wells and incubated at room temperature for 15 minutes. A 100 µL of basal medium with supplement was added on top of the hydrogel. The cultures were incubated overnight, and the medium was changed every 2-3 days. After two weeks, the tumoroids were then subjected to Cyto3D® Live-Dead staining and carefully transferred to the VitroPrime™ Spread Attach 96-well plate. Acridine orange (AO) staining indicates the presence of live cells within the tumoroid as shown in green. Propidium iodide (PI; in red) stains for dead cells. Images were taken with the Keyence BZX microscope system at 4X (top) and 10X (bottom) magnifications.

Quantity

8 Packs, 8 Packs x 5