Data and References

Figure 1. Spheroid invasion assay using the VitroPrime™ Ultra-low Attachment Plate and VitroGel® Hydrogel Matrix
U87-MG glioblastoma cells were resuspended in basal medium with 10% fetal bovine serum. Twenty microliters (20 µL) of cell suspension were added to the VitroPrime™ Ultra-low Attachment Plate, U-bottom, 96-well plate. The cultures were incubated overnight at 37°C to allow spheroid formation. VitroGel® Hydrogel Matrix was combined with serum, and 40 µL of the mixture was added to the spheroid, followed by a 15-minute incubation at room temperature. The images were obtained with the Zeiss Microscope at a 2.5X magnification.

Figure 2. EMT GBM Tumoroid Viability
U87-MG GBM cells (1 x 10^6 cells/mL) were resuspended in basal medium with supplement system. Twenty microliters (20 µL) of cell suspension were added to the VitroPrime™ Ultra-low Attachment Plate, U-bottom, 96-well plate. The cultures were incubated overnight at 37 °C for spheroid formation. The hydrogel (40 µL) was added to the wells and incubated at room temperature for 15 minutes. A 100 µL of basal medium with supplement was added on top of the hydrogel. The cultures were incubated overnight, and the medium was changed every 2-3 days. After two weeks, the tumoroids were then subjected to Cyto3D® Live-Dead staining and carefully transferred to the VitroPrime™ Spread Attach 96-well plate. Acridine orange (AO) staining indicates the presence of live cells within the tumoroid as shown in green. Propidium iodide (PI; in red) stains for dead cells. Images were taken with the Keyence BZX microscope system at 4X (top) and 10X (bottom) magnifications.