VitroGel® Hydrogel Matrix is a ready-to-use, xeno-free (animal origin-free) functional hydrogel for 3D cell culture research. VitroGel Hydrogel Matrix is an optimized formulation of multi-functional ligands and concentration to support a wide range of cell types for different applications.
“Just Add Cells” – The hydrogel matrix is ready to mix with cell suspension directly. There is no additional adjustment needed.
VitroGel Hydrogel Matrix closely mimics the natural extracellular matrix (ECM) environment to make cells feel more like at home. The hydrogel is room temperature stable, has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution.
This user-friendly functional hydrogel creates an excellent balance of simplicity and versatility.
|Formulation||Xeno-free, functional hydrogel|
|Use||3D and 2D cell culture|
|Operation||Ready-to-use at room temperature|
|Biocompatibility||Biocompatible, safe for animal studies|
|Injection||Injectable hydrogel for in vivo studies and lab automation|
|Cell Harvesting||20 min cell recovery using VitroGel Cell Recovery Solution|
|Storage||Store at 2-8°C. Ships at ambient temperature|
|Sizes||10 mL and 2 mL|
|Usage||300 uses (10 mL), 60 uses (2mL)|
Recover cells from the hydrogel in 20 minutes with high cell viability. Enzyme-free system.
Handbooks and Resources
Data and References
3D Cell Model
Figure 1. Human mammary breast cancer cells cultured in VitroGel Hydrogel Matrix and Matrigel, a natural Extracellular Matrix (ECM) hydrogel
VitroGel Hydrogel Matrix can support the growth of various cell types. The images above are 3D cell culture of human mammary breast cancer cells (MCF-7) at day 7 in VitroGel Hydrogel Matrix and Matrigel®, a natural ECM-based hydrogel from Engelbreth-Holm-Swarm murine sarcoma. The cells were prepared as single cells suspension and encapsulated within both hydrogels. The grape-shaped like cell colonies appears on day 1 for both hydrogels. However, cells displayed 3D luminal structures (see arrows) only with VitroGel Hydrogel Matrix. The cells cultured in Matrigel can only perform the spheroid structure. (Z-stack imaging system with 2D image projection was used. blue: DAPI; green: actin)
Figure 2. Bone marrow cells 3D cultured in VitroGel Hydrogel Matrix and Matrigel, a natural Extracellular Matrix (ECM) hydrogel
The fibroblast-like mouse bone marrow stromal cells (OP9-GFP) were 3D cultured in VitroGel Hydrogel Matrix and Matrigel®, a natural ECM-based hydrogel from Engelbreth-Holm-Swarm murine sarcoma. Single cells were homogeneously suspended within each hydrogel and both start to form stretched fibroblast-like structure on day 1. The images above show a clear 3D cellular networking structure formed in both hydrogels on day 7. Compared to Matrigel, the multiple functional ligands in the VitroGel Hydrogel Matrix promote a stronger cell-matrix interaction, which helps accelerate the cell proliferation and cell-cell communications during the 3D cell culture.
Matrigel is a trademark of Corning Incorporated.
- Powell K. Adding depth to cell culture. Science, 356(6333), 96–98. https://doi.org/10.1126/science.356.6333.96