VitroGel® Hydrogel Matrix

ready-to-use, xeno-free (animal origin-free) functional hydrogel system

  • 2 mL
  • 10 mL
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Overview

VitroGel® Hydrogel Matrix is a ready-to-use, xeno-free (animal origin-free) functional hydrogel for 3D cell culture research. VitroGel Hydrogel Matrix is an optimized formulation of multi-functional ligands and concentration to support a wide range of cell types for different applications.

“Just Add Cells”The hydrogel matrix is ready to mix with cell suspension directly. There is no additional adjustment needed.

VitroGel Hydrogel Matrix closely mimics the natural extracellular matrix (ECM) environment to make cells feel more like at home. The hydrogel is room temperature stable, has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution.

This user-friendly functional hydrogel creates an excellent balance of simplicity and versatility.

Specifications

FormulationXeno-free, functional hydrogel
Use3D and 2D cell culture
OperationReady-to-use at room temperature
BiocompatibilityBiocompatible, safe for animal studies
InjectionInjectable hydrogel for in vivo studies and lab automation
Cell Harvesting20 min cell recovery using VitroGel Cell Recovery Solution
pHNeutral
StorageStore at 2-8°C. Ships at ambient temperature
Sizes10 mL and 2 mL
Usage300 uses (10 mL), 60 uses (2mL)

3D cell culture process in 20 min – “Just add cells”

VitroGel Hydrogel Matrix is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.

Recommended Product

Recover cells from the hydrogel in 20 minutes with high cell viability. Enzyme-free system.

VitroGel Cell Recovery Solution

Handbooks and Resources

Video Protocols

VIDEO PROTOCOL TIP

VIDEO PROTOCOL TIP

Data and References

3D Cell Model

Figure 1. Human mammary breast cancer cells cultured in VitroGel Hydrogel Matrix and Matrigel,  a natural Extracellular Matrix (ECM) hydrogel

VitroGel Hydrogel Matrix can support the growth of various cell types. The images above are 3D cell culture of human mammary breast cancer cells (MCF-7) at day 7 in VitroGel Hydrogel Matrix and Matrigel®, a natural ECM-based hydrogel from Engelbreth-Holm-Swarm murine sarcoma. The cells were prepared as single cells suspension and encapsulated within both hydrogels. The grape-shaped like cell colonies appears on day 1 for both hydrogels. However, cells displayed 3D luminal structures (see arrows) only with VitroGel Hydrogel Matrix. The cells cultured in Matrigel can only perform the spheroid structure. (Z-stack imaging system with 2D image projection was used. blue: DAPI; green: actin)

Figure 2. Bone marrow cells 3D cultured in VitroGel Hydrogel Matrix and Matrigel, a natural Extracellular Matrix (ECM) hydrogel

The fibroblast-like mouse bone marrow stromal cells (OP9-GFP) were 3D cultured in VitroGel Hydrogel Matrix and Matrigel®, a natural ECM-based hydrogel from Engelbreth-Holm-Swarm murine sarcoma. Single cells were homogeneously suspended within each hydrogel and both start to form stretched fibroblast-like structure on day 1. The images above show a clear 3D cellular networking structure formed in both hydrogels on day 7. Compared to Matrigel, the multiple functional ligands in the VitroGel Hydrogel Matrix promote a stronger cell-matrix interaction, which helps accelerate the cell proliferation and cell-cell communications during the 3D cell culture.

Matrigel is a trademark of Corning Incorporated.

References/Publications

Size

2 mL, 10 mL