VitroGel® ORGANOID are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs).
VitroGel ORGANOID hydrogels are ready-to-use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.
The hydrogels can work together with VitroGel STEM (Cat# VHM02), a hydrogel system for 3D static suspension cultures and scale-up of human pluripotent stem cells, by transferring the stem cell spheroids from VitroGel STEM to VitroGel ORGANOID hydrogels for organoid differentiation. The key growth factors and molecules can directly mix with the hydrogel matrix or add on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution. VitroGel ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.
The VitroGel ORGANOID Discovery Kit (Cat# VHM04-K) includes all four types of organoid hydrogels (VitroGel ORGANOID-1, VitroGel ORGANOID-2, VitroGel ORGANOID-3, VitroGel ORGANOID-4) which were formulated with various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions.
|Formulation||Xeno-free, functional hydrogel|
|Operation||Ready-to-use at room temperature|
|Biocompatibility||Biocompatible, safe for animal studies|
|Injection||Injectable hydrogel for in vivo studies and lab automation|
|Cell Harvesting||Easy cell recovery using VitroGel Cell Recovery Solution|
|Storage||Store at 2-8°C. Ships at ambient temperature|
|Sizes||Single Vial: 10 mL
Discovery Kit: (2 mL) each of ORGANOID-1, ORGANOID-2, ORGANOID-3, ORGANOID-4
|Usage||60 uses for 2 mL bottle at 50 µL/test
300 uses for 10 mL bottle at 50 µL/test
Recover cells from the hydrogel in 20 minutes with high cell viability. Enzyme-free system.
Handbooks and Resources
Data and References
Figure 1. Mouse intestinal organoid culture on VitroGel ORGANOID and Matrigel.
Images show the growth of mouse intestinal organoid from day 0 to day 14.
Figure 2. Organoid culture from hPSCs spheroids
Human pluripotent stem cells (hPSCs) were cultured and maintained as stem cell spheroids in VitroGel STEM for 6 days. On day 6, the stem cell spheroids were harvested from VitroGel STEM and resuspend in advanced DMEM/F-12 medium with B27 and N2 supplement. The stem cell spheroids were then encapsulated in VitroGel ORGANOID Hydrogel (VitroGel ORGANOID-1) by mixing the hydrogel and cells at a 2:1 ratio (v/v). After 10-15 minutes, the stem cell culture medium was added on top of the hydrogel for overnight. The top medium was replaced with a stem cell differentiation medium on day 1. On Day 3, the top medium was replaced with an organoid transfer medium. The organoid maintaining medium was added directly to the top of the hydrogel on day 4 to make a 50/50 mixture of organoid transfer medium and organoid maintaining medium. On day 5, replace the top medium to 100% organoid maintaining medium for long term culture. Change 50-80% of the top medium every other day or whenever the medium turn to yellow color.
Figure 3. Growth of organoids after adding organoid medium
Figure 4. Organoid formation on day 10 after seeding in VitroGel ORGANOID hydrogel
- Powell K. Adding depth to cell culture. Science, 356(6333), 96–98. https://doi.org/10.1126/science.356.6333.96