Protocols and Resources
RocketCell™ iPSC Xeno-Free Growth Medium – Protocol
Product Documentation
RocketCell™ iPSC Xeno-Free Growth Medium – Sale Sheet
Product Data Sheet
New
RocketCell™ iPSC Xeno-Free Growth Medium
Xeno-free medium for 3D expansion of pluripotent stem cells
$309.00
RocketCell™ iPSC Xeno-Free Growth Medium
Xeno-free medium for 3D expansion of pluripotent stem cells
RocketCell™ iPSC Xeno-Free Growth Medium is designed to support the robust growth and maintenance of human induced pluripotent stem cells (iPSCs) under xeno-free conditions. Engineered with defined components and optimized nutrient balance, this medium ensures consistent cell performance, stable pluripotency, and high viability across passages.
RocketCell™ iPSC Xeno-Free Growth Medium provides a reliable solution for researchers seeking a feeder-free, animal-component-free culture environment for iPSC expansion and differentiation studies. The formulation minimizes variability and supports efficient cell attachment, rapid proliferation, and healthy colony morphology, making it ideal for regenerative medicine and disease modeling.
Kit Contents:
- 1 x 500 mL, RocketCell™ iPSC Xeno-Free Basal Medium
- 1 x 10 mL, RocketCell™ iPSC Xeno-Free Supplement (50X)
For an all-in-one optimized kit for 3D expansion of pluripotent stem cells, use
RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit↗
Specifications
| Use | 3D pluripotent stem cell cultures |
| Shipping and Storage | Basal Medium: Ships with ice pack. Store at 2-8°C. Supplement: Ships with dry ice Store at -20°C. |
Data and References
3D: CASE 1
Indirect Immunofluorescence staining of IPSCs Grown
in VitroGel® STEM Hydrogel
Pluripotency is maintained by RocketCell™ 3D iPSC Xeno-Free Growth Medium
Figure 1: Indirect Immunofluorescence of Pluripotency Marker on IPSCs Grown using RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit.
iPSCs (100k/well) were grown for 7 days in 24-well VitroPrime™ 3D imaging plates, fixed, and stained with pluripotency markers Tra-1-60, Lin28, and Oct4 using Alexa Fluor 488, 594, and 647 secondary antibodies. Images were captured using a Leica MICA confocal microscope. These results confirm that the RocketCell™ 3D iPSC Growth Kit provides a supportive 3D microenvironment for maintaining pluripotency.
3D: CASE 2
Direct Immunofluorescence staining of IPSCs Grown
in VitroGel® STEM Hydrogel
Pluripotency is maintained by RocketCell™ 3D iPSC Xeno-Free Growth Medium
Figure 2: Direct Immunofluorescence Staining of IPSCs (HFF-1VL, TheWell Bioscience) Grown in RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit.
iPSCs (100k/well) were grown for 7 days in 24-well VitroPrime™ 3D imaging plates before fixation and staining with directly labeled pluripotency markers Lin28 (Alexa 488), Podocalyxin (Alexa 594), and Nanog (Alexa 647). Images were captured using a Leica MICA confocal microscope. These results show that the RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit provides a supportive 3D environment for maintaining pluripotent stem cells, while the use of directly labeled antibodies enables faster and more efficient hydrogel-based sample processing.
Resources
Enhance your stem cell culture with these products:
Hydrogels - Ready-To-Use
ready-to-use, xeno-free (animal origin-free) hydrogel system for hPSCs 3D static suspension culture and scale-up
New
Cell Culture Medium (Xeno-Free)
An all-in-one optimized kit for 3D expansion of pluripotent stem cells
Cell Harvesting Solution
Non-enzymatic cell harvesting solution to recover cells/organoids from hydrogel or an animal-based ECM within 15 minutes. Improved formulation over VitroGel® Cell Recovery Solution.
Biomarkers
Versatile, live/dead cell viability analysis for 3D and 2D cell culture. IN STOCK
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Data and References Figure 1. Use of VitroGel® hydrogels to generate iPSC derived intestinal organoids. Representative image showing iPSC derived intestinal organoids cultured in VitroGel® ORGANOID. iPSC cells were cultured in VitroGel® STEM to generation of spheroids cells. The spheroids were then differentiated to intestinal organoids using CytoGrow™ growth factors. Yellow fluorescence: Phalloidin, Magenta: Villin [...]
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Data and References
