Xeno-free 3D Tumoroid Platform
Complete animal-free system for 3D GBM tumoroid generation with batch-to-batch consistency.
NEW
VitroGel® Glioblastoma (GBM) Xeno-Free EMT Kit
Ready-to-use kit for 3D GBM tumoroid formation.
Supports epithelial-to-mesenchymal transition with long-term tumoroid culture.
Hydrogel can be purchased separately. Click here
VitroGel® Glioblastoma (GBM) Xeno-Free EMT Kit
Ready-to-use kit for 3D GBM tumoroid formation, supporting epithelial-to-mesenchymal transition and enabling long-term tumoroid culture.
Simple Workflow at Room Temperature
Generate the tumoroid from a simple, single spheroid with an easy operating protocol at room temperature.
Enables EMT Process and Long-Term Culture
Supports the biologically relevant epithelial-to-mesenchymal transition and maintain the well-organized structure for months.
Automation-Friendly and HTS
Compatible with lab-automated liquid handling systems and supports high-throughput screening process.
The VitroGel® Glioblastoma (GBM) Xeno-Free EMT Kit is an excellent tool for generating 3D GBM tumoroids, enabling cells to undergo the epithelial-to-mesenchymal transition for examining pathways involved in cancer metastasis, drug screening, and high-throughput automation.
The kit features the ready-to-use and synthetic VitroGel® EMT hydrogel, RocketCell™ GBM Xeno-Free EMT supplement (50X), and VitroPrime™ Ultra-Low Attachment, U-Bottom, 96-Well Plate.
The VitroGel® Glioblastoma (GBM) Xeno-Free EMT Kit is designed to induce tumoroid formation from a simple single spheroid to generate a biologically relevant and well-organized tumoroid model for studying tumor progression, EMT dynamics, and therapeutic response in a preclinical setting.
VitroGel® Glioblastoma (GBM) Xeno-Free EMT Kit (VHM08-K)
Contents:
- 1 x 2 mL, VitroGel® EMT hydrogel
- 1 x 5 mL, RocketCell™ GBM Xeno-Free EMT supplement (50X)
- 1 x VitroPrime™ Ultra-Low Attachment Plate, U-Bottom, 96-Well
VitroGel® Glioblastoma (GBM) Xeno-Free EMT High-Screening Kit
(VHM08-HK) Contents:
- 5 x 2 mL, VitroGel® EMT hydrogel
- 5 x 5 mL, RocketCell™ GBM Xeno-Free EMT supplement (50X)
- 5 x VitroPrime™ Ultra-Low Attachment Plate, U-Bottom, 96-Well
Specifications
| Use | 3D Glioblastoma tumoroid formation for drug screening purposes |
| Formulation | Xeno-free, biofunctional hydrogel and supplement system |
| Injectability | Injectable hydrogel for in vivo studies and lab automation |
| Operation | Ready-to-use at room temperature |
| Cell Harvesting | VitroGel® Organoid Recovery Solution 5-15 min cell recovery |
| Shipping and Storage | Hydrogel: Ships at ambient temperature. Store at 2-8°C. Supplement: Dry Ice shipping. Store at -20°C. |
Challenges We Solve
How VitroGel® GBM Xeno-Free EMT Kit
Common Challenges in 3D Tumoroid Generation
Lack of a robust 3D EMT tumoroid model tounderstand cancer metastasis
Hard to support a long-term
tumoroid cultures
Lack of a method to establish comprehensive tumoroid models from well characterized cell lines
Difficult to adapt lab-automation and high-throughput screening for big-sized 3D tumoroids
How VitroGel® GBM Xeno-Free EMT Kit
Solves The Issues
Induce a controllable EMT process for tumoroid formation
The unique combination of VitroGel® EMT hydrogel and RocketCell™ EMT supplement induces the epithelial-to-mesenchymal transition for glioblastoma cells.
Long-term Culture
Over 6 weeks of tumoroid maintenance for prolonged studies.
Simple, Fast, and Reliable
Establish tumoroid formation from well-established cell lines in less than a week with highly reproducible results.
Automation-friendly
Room temperature operation makes an easy transition to high-throughput platforms.
Protocols and Resources
Product Documentation
VitroGel® Glioblastoma (GBM) Xeno-Free EMT Kit – Sale Sheet
Product Data Sheet
Data and References
CASE 1
VitroGel® Hydrogel and Defined Supplement System Promotes Tumoroid Formation
Figure 1: GBM tumoroid development in VitroGel® EMT hydrogel system. U87-MG GBM cells (1 x 106 cells/mL) were resuspended in basal medium with supplements. 20 µL of cell suspension was added to the VitroPrime™ Ultra-Low Attachment, U-Bottom, 96-Well Plate and incubated overnight at 37°C. VitroGel® EMT hydrogel was mixed with RocketCell™ GBM Xeno-Free EMT supplement in a 1:1 ratio, and 40 µL of the mixture was added to the cultures. The hydrogel was incubated at room temperature for 15 minutes. A 100 µL of basal medium with supplements was added on top of the hydrogels. In parallel, Matrigel was diluted 1:10 with basal medium containing FBS. The cultures were incubated at 37°C. Tumoroid growth was monitored for 25 days and evaluated using a Zeiss microscope.
CASE 2
3D Tumoroid formation with the VitroGel® Glioblastoma Xeno-Free EMT Kit
Figure 2: VitroGel® Glioblastoma Xeno-Free EMT kit supports tumoroid formation.
U87 MG cells were resuspended in basal medium supplemented with 1X RocketCell™ GBM Xeno-Free EMT supplement and seeded into the VitroPrime™ Ultra-Low Attachment Plate, U-Bottom, 96-Well, to promote overnight spheroid formation. The spheroids were supplemented with VitroGel® EMT and RocketCell™ GBM Xeno-Free EMT supplement to induce tumoroid generation. Light microscopy was performed multiple days to evaluate tumoroid growth over time (left). Cyto3D® Live-Dead Assay staining was performed on GBM tumoroids. Live cells within the tumoroid are shown as green and dead cells in red (right).
CASE 3
Evaluation Viability of GBM Tumoroids
Figure 3: VitroGel® Glioblastoma Xeno-Free EMT kit hydrogel sustains tumoroid growth and viability.
GBM tumoroids were subjected to cell viability staining using Cyto3D® Live-Dead Assay Kit after six weeks in culture. Live cells within the tumoroid are shown in green, and dead cells are shown in red.
CASE 4
Tumoroids grown with the VitroGel® Glioblastoma Xeno-Free EMT Kit
as a Platform for Drug Screening
Figure 4: GBM tumoroids are susceptible to chemotherapy.
Tumoroids were grown for 3 days in VitroGel® EMT hydrogel with RocketCell™ supplement system. The tumoroids were treated with Temozolomide (TMZ; 1mM) for 24 hours and subjected to cell viability studies. The Cyto3D® Live-Dead Assay Kit was used to label live cells (green) and dead cells (red) in the tumoroid, both with and without drug treatment. The pictures were obtained at a 20X magnification.
CASE 5
Tumoroid Characterization: Evaluation of EMT Markers
Figure 5: Assessing Vimentin expression, an EMT marker, in GBM tumoroids.
Immunofluorescence staining was employed on three-week-old tumoroids to examine Vimentin expression. The nuclei were stained with DAPI (blue), and vimentin was stained green. A. Representative image of tumoroid topology and size (4X magnification). B-C. Enlarged images of vimentin-positive regions obtained with a confocal microscope at 10X magnification.
CASE 6
VitroGel® EMT hydrogel and Xeno-Free Supplement Maintain Co-Culture Viability
Figure 6: Assessing the viability of GBM tumoroids and endothelial cells co-cultures after exposure to chemotherapy.
U87 cells and endothelial cells were combined in a 1:1 ratio and seeded onto the VitroPrime™ Ultra-Low Attachment, U-Bottom, 96-Well Plate for spheroid formation. The next day, VitroGel® EMT hydrogel was supplemented with FBS or the Xeno-Free supplement and added to the spheroids to form tumoroids. The tumoroids were grown for 21 days and then subjected to TMZ (1mM) for 24 hours. After exposure, the Cyto3D® LiveDead Assay Kit was used to evaluate viability.
CASE 7
GBM Tumoroid and Endothelial Cell Co-Culture: Adding ECs after Spheroid Formation
Figure 7. Co-culture model of GBM cells and Endothelial cells for drug-screening studies.
U87 cells were seeded on to the VitroPrime™ Ultra-Low Attachment, U-Bottom, 96-Well Plate for spheroid formation and incubated overnight. Next, VitroGel® EMT hydrogel was supplemented with FBS or Xeno-Free supplement and added to the spheroids for tumoroid formation. Then, endothelial cells were added on D1 to establish co-cultures. The tumoroids were grown for 21 days and then subjected to TMZ (1mM) for 24 hours, followed by viability assessment.
Resources
| GBM | Hydrogel only, Supplement only, Standard Kit, High-Screening Kit |
|---|
Related products
Angiogenesis Assay Kits
ready-to-use, hydrogel system for 2D gel coating and 3D culture of angiogenesis tube formation, invasion, and animal injection.
Angiogenesis Assay Kits
Tunable hydrogel system for 2D gel coating and 3D culture of angiogenesis tube formation, invasion, and animal injection.
Assay Kits
A simple and easy replacement for animal-based ECM for consistent cell invasion studies. This kit includes the VitroGel® Hydrogel Matrix with VitroPrime™ Cell Culture Inserts, 8 µm, PET
Xeno-free hydrogel system for consistent study of cell invasion. Kit includes choice of tunable functionalized hydrogel, 10mL VitroGel® Dilution Solution TYPE 2, and VitroPrime™ Cell Culture Inserts, 8 µm.
