RocketCell™ NSC Xeno-Free Growth Medium
Xeno-Free complete medium for 3D and 2D neural stem cell culture
RocketCell™ NSC Xeno-Free Growth Medium
Xeno-Free complete medium for 3D and 2D neural stem cell culture
The RocketCell™ NSC Xeno-Free Growth Medium is designed to support the expansion and maintenance of neural stem cells (NSCs) in both 3D and 2D formats.
This medium provides a robust, scalable platform for long-term culture and large-scale expansion of undifferentiated NSCs in both 3D and 2D formats—while maintaining their multipotency and functional integrity. Designed for convenience and performance, this system integrates seamlessly with high-resolution imaging and high-throughput screening workflows for advanced neuroscience and regenerative medicine research.
Contents:
- 1 x 500 mL, RocketCell™ NSC Xeno-Free Basal Medium
- 1 x 10 mL, RocketCell™ NSC Xeno-Free Supplement (50X)
- 1 x 10 µg CytoGrow™ Recombinant EGF (Human), CHO, Tag Free
- 1 x 10 µg, CytoGrow™ Recombinant bFGF/FGF-2 (Human), E. coli, Tag Free
Also Available:
- RocketCell™ 3D NSC Xeno-Free Complete Growth Kit↗
An all-in-one, ready-to-use kit for 3D neural stem cell culture.
Specifications
| Use | 3D and 2D proliferation and maintenance of neural stem cells |
| Shipping and Storage | Basal Medium: Ships with ice pack. Store at 2-8°C. Supplement Dry Ice shipping. Store at -20- -80°C. Growth Factors: Ships with Ice pack/Dry ice. Store at -20- -80°C. |
Recommended Products
VitroPrime™ Spread-Attach Plate
Supports neuron growth. No PDL-coated plates required.
Common Challenges in
NSC Culture
Lack of a complete xeno-free platform for 3D and 2D NSC Culture
Spontaneous differentiation in
animal-based ECMs
Poor cell matrix and cell-cell interaction for 3D neurite axonal outgrowth
Lack of an optimal combination of matrix and xeno-free complete medium for NSC culture
Difficult to perform long-term NSC Culture
How RocketCell™ NSC Xeno-Free Complete Growth Kit Solves The Issues
Supports 3D NSC Culture
Enables both single cells and 3D spheroids to grow within VitroGel® NEURON hydrogel.
Improved Cell Attachment for 2D NSC Culture
Use 4X less concentration than animal-based ECM to support a fast and reliable coating method at room temperature.
Enhanced 3D Cell Connectivity and Network Formation
Robust network formation and axonal projections mimicking the in vivo environment.
Complete all-in-one package
Optimal xeno-free combination of hydrogel matrix and culture medium for robust and reliable results every time.
Long-Term Culture and Negligible Spontaneous Differentiation
Over 4 weeks in culture without compromising multipotency in both 2D and 3D
Protocols and Resources
RocketCell™ NSC Media for 2D Applications – Instruction Manual
Product Documentation
RocketCell™ NSC Xeno-Free Growth Medium – Sale Sheet
Product Data Sheet
Frequently Asked Questions Data and References
2D THIN COATING METHOD
2D: CASE 1
2D NSC Maintenance (1:200 coating on VitroPrime™ Spread-Attach Plate)
A.
B.
Figure 1. VitroGel® NEURON 2D Thin Coating for Growth of iPSC-Derived NSCs and Confluence Analysis on VitroPrime™ Spread-Attach Plates.
A. iPSC-derived NSCs (CD34-eIPSC-NSC) were plated at 50,000 cells per well on 24-well VitroPrime™ Spread-Attach Plates coated with either VitroGel® NEURON (1:200) or Geltrex®, and cultured for 6 days. Images were captured using the Incucyte® S3 system. B. NSC growth was quantified by percent confluence over time, with images collected every 8 hours and analyzed relative to the initial timepoint (T = 0 + 4 hrs).
2D: CASE 2
2D NSC Maintenance and Characterization
(1:200 coating on VitroPrime™ Spread-Attach Plate)
Figure 2. NSC Characterization after Subculturing with the RocketCell™ NSC Xeno-Free Growth Medium.
iPSC-derived NSCs (CD34-eIPSC-NSC) were plated at 50,000 cells per well on 24-well VitroPrime™ Spread-Attach Plates coated with VitroGel® NEURON (1:200). Immunofluorescence staining depicts the presence of cells positive for Nestin (A, green) and Sox2 (B, red), which are NSC markers. The nuclei were stained with DAPI (A, blue) or Hoescht (B, blue).
3D: CASE 1
3D Characterization of Differentiated NSC Spheroids
Figure 3. RocketCell™ NSC Xeno-Free Growth Medium supports the expansion of NSC spheroids for differentiation.
iPSCs were 3D cultured in RocketCell™ 3D Xeno-Free IPSC Xeno-Free Growth Kit and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Xeno-Free Growth Medium. The cells were then differentiated into neurons with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The samples were fixed and stained to evaluate the expression of (A) Microtubule-associated protein 2 (MAP2; green), (B) Neurofilament-H (red), and (C) Synaptophysin (purple). The nuclei were stained with Hoescht dye.
Enhance your neuron cell culture with these products:
Cell Culture Media (Xeno-Free)
All-in-one, ready-to-use kit for 3D neural stem cell culture.
CytoGrow™ - Recombinant Proteins
Premium CytoGrow™ growth factor suitable for cell culture supplementation, wound healing research, epithelial biology, and cancer signaling studies.
3D Culture Vessels
Unique surface treated for superior hydrogel spreading, adherence, and uniform surface.
Cell Harvesting Solution
Non-enzymatic cell harvesting solution to recover cells/organoids from hydrogel or an animal-based ECM within 15 minutes.
Downstream Cell Analysis
Fast (15 min), versatile, live/dead cell viability analysis for 3D and 2D cell culture. IN STOCK
| Kit | 1 Kit |
|---|
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