Category Archives: Video Protocols – 3D cell culture

HEK293 3D Suspension Cell Harvesting

VitroGel® HEK293 is a xeno-free (animal origin-free) functional hydrogel system developed to support three-dimensional (3D) cultures of human embryonic kidney 293 (HEK293) cells. Easily harvest HEK293 cells in less than 15 minutes with our VitroGel Cell Recovery Solution.

3D Organoid Harvesting

Recovering organoids from 3D hydrogel system is challenging. In some cases, the process can take hours using either harsh chemical solutions, strong enzyme, or temperature adjustment, which can damage cells and reduce the yield.​ ​With VitroGel® ORGANOID, recovering organoids from the hydrogel matrix is now simple, fast and efficient. Using our enzyme-free VitroGel® Cell Recovery Solution, […]

3D Organoid Culture with VitroGel ORGANOID

VitroGel® ORGANOID are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs). Organoids are further easily harvested with our enzyme-free cell recovery solution, VitroGel Cell Recovery Solution.

2D Coating Culture with VitroGel “Ready-To-Use” Hydrogels

VitroGel® ready-to-use hydrogels simplify the hydrogel 3D cell culture procedure from hours to minutes. Just mix with your cells. There is no crosslinking agent or the need to adjust the hydrogel concentration. The whole 2D coating protocol can be done in 20 minutes (including a 10-15 min waiting time for hydrogel stabilization). The room temperature […]

3D Cell Culture with VitroGel Ready-To-Use Hydrogels

VitroGel® ready-to-use hydrogels simplify the hydrogel 3D cell culture procedure from hours to minutes. Just mix with your cells.  There is no crosslinking agent or the need to adjust the hydrogel concentration. The whole 3D cell culture process can be done in 20 min including a 10-15 min waiting time for hydrogel stabilization. The room […]

MSC (Mesenchymal Stem Cell) 3D Culture Scale-Up | Bead-Droplet Protocol

VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs). The Bead-Droplet method can be used to make hydrogel cell beads for MSC scale-up and long-term culture. This novel culture method generates high-quality MSCs and eliminates the usage of microcarriers for MSC scale-up.