CytoGrow™ Recombinant bFGF/FGF-2 (Human), E. coli, Tag Free
Specifications
Synonym: | Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF |
Species: | Human |
Expression System: | E. coli |
Endotoxin: | ≤0.1 EU/mg |
Appearance: | White powder, colorless liquid after reconstitution |
Formulation | Lyophilized from a 0.22 μm filtered solution containing PBS, 5% mannitol, and 0.01% Tween 80 |
Reconstitution: | Recommended to redissolve in sterile deionized water |
Shipping: | Ships overnight |
Storage and Stability: | Store in a manual defrost freezer and avoid repeated freeze-thaw cycles. Lyophilized state: 36 months at -20°C to -80°C After reconstitution and under sterile conditions: 6 months at -20°C to -80°C 7-10 days at 2°C to 8°C |
Bioactivity

Data

Figure 1. CytoGrow™ growth factors support 3D encapsulation culture of NSC cells as single cell suspension in VitroGel® NEURON using RocketCell™ NSC Xeno-Free Growth Medium.
VitroGel® NEURON Hydrogel and RocketCell™ NSC Xeno-Free Media Support 3D NSC Cultures and Neurite Outgrowth. The growth medium was supplemented with CytoGrow EGF and FGF2. 3D NSC cultures were established by preparing a single-cell NSC suspension with RocketCell™ NSC Xeno-Free Medium and mixing with VitroGel NEURON for 6 days (A). Immunofluorescence staining was performed to evaluate the presence of beta-III-tubulin, a neuron maturation marker. The images were obtained using the Keyence BZ-X microscope at 10x and 20x magnifications (B).