3D iPSC Culture: RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit – An all-in-one kit with optimized matrix, medium, and reagents, for 3D iPSC expansion.

On-demand webinar

PRESENTER

Rick Cohen, Ph.D. 

TheWell Bioscience
Principal Scientist

WATCH THE WEBINAR

Learn how an integrated, xeno-free platform with optimized matrix, medium, and reagents can accelerate your path to reproducible, clinically aligned 3D iPSC models.

Induced pluripotent stem cells (iPSCs) provide a powerful foundation for disease modeling, organoid development, and cell therapy applications. However, most iPSC research is still performed in two-dimensional (2D) systems that fail to fully recapitulate native tissue architecture and cellular interactions.

While three-dimensional (3D) iPSC culture and scale-up have been widely discussed as essential next steps for advanced cell modeling and regenerative medicine, the lack of standardized, integrated workflows has remained a major barrier. Researchers are often required to source, optimize, and validate disparate matrices, media, and supplements —resulting in high variability, limited reproducibility, and significant time investment.

In this webinar, we introduce the RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit—an all-in-one, fully defined platform designed to simplify and standardize 3D iPSC culture and expansion. This integrated system combines a synthetic VitroGel® STEM hydrogel matrix with optimized xeno-free media, supplements, and a cell viability enhancer, eliminating the need for fragmented workflows and extensive optimization.

We will demonstrate a step-by-step approach for seamlessly transitioning iPSCs from 2D to 3D culture using this unified platform. Attendees will learn how to establish robust, reproducible 3D iPSC cultures that maintain pluripotency, long-term viability, and scalability with 10X cell density compared to the traditional culture method. We will also highlight compatibility with routine assays, immunostaining, and high-resolution 3D imaging workflows.

Supported by validated protocols and multi-line iPSC data, this presentation will show how the RocketCell™ platform enables researchers to move beyond trial-and-error and adopt a standardized foundation for downstream organoid generation, multilineage differentiation, and translational applications.

KEY TAKEAWAYS

  • 3D, xeno-free iPSC culture provides a more physiologically relevant foundation for disease modeling, organoid generation, and translational research compared to traditional 2D systems.
  • The RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit delivers a standardized, all-in-one solution that supports high-density expansion while maintaining pluripotency, long-term viability, and reproducibility.
  • All-in-one kit, end-to-end workflows eliminate trial-and-error, enabling researchers to transition from 2D to 3D culture quickly and confidently.
  • Scalable, imaging-compatible 3D platforms accelerate downstream applications, including multilineage differentiation, functional assays, and clinically aligned development.

PRESENTER

Dr. Rick Cohen is a stem cell biologist with more than two decades of experience in stem cell research, iPSC technologies, and advanced cell culture systems. He received his doctorate in Pharmacology and Therapeutics from McGill University and completed postdoctoral training in neurogenetics at the NIH (NINDS).

Over the course of his career, Dr. Cohen has established standardized stem cell culture protocols, developed viral and non-integrating methods for iPSC generation, and engineered functional differentiation systems across multiple lineages. He has trained more than 100 scientists in human stem cell techniques and has contributed extensively to stem cell biology, including work on iPSC expansion, neuronal differentiation, and scalable culture systems.

His expertise spans both foundational stem cell biology and translational applications, with a strong focus on developing reproducible and physiologically relevant culture platforms.

PRODUCT USED:

RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit

RELATED PRODUCTS:

RocketCell™ iPSC Xeno-Free Growth Medium

VitroPrime™ 3D Culture and Imaging Plate 

Need help with stem cell culture?