RocketCell™ 3D NSC Xeno-Free Complete Growth Kit

All-in-one, ready-to-use kit for 3D neural stem cell culture

Ready-to-use, All-in-one Kit
100% synthetic kit with optimal combination of matrix and medium for 2D and 3D NSC culture and maintenance.

Supports 3D NSC Network Formation
Promotes 3D neurite and axonal outgrowth for 3D networking formation, ideal for advanced studies.

Long-Term Culture and Maintenance
Culture NSCs for over 4 weeks without compromising their multipotency. 

Simple 3D Workflow at Room Temperature
Room temperature operation with simple mixing or coating steps; supports lab automation. 

Easy Cell Harvesting
Simple and efficient cell harvesting with VitroGel® Organoid Recovery Solution

The RocketCell™ NSC Xeno-Free Complete Growth Kit is an all-in-one, ready-to-use kit designed to support the expansion and maintenance of neural stem cells (NSCs) in both 3D and 2D formats. This kit offers a fully defined, optimized combination of a synthetic hydrogel and a xeno-free complete medium, eliminating variability and ensuring consistent, reproducible results.

The RocketCell™ NSC Xeno-Free Complete Growth Kit provides a robust, scalable platform for long-term culture and large-scale expansion of undifferentiated NSCs in both 3D and 2D formats—while maintaining their multipotency and functional integrity. Designed for convenience and performance, this ready-to-use system operates at room temperature, integrates seamlessly with high-resolution imaging and high-throughput screening workflows for advanced neuroscience and regenerative medicine research.

The all-in-one kit includes:

3D Cell Culture:
RocketCell™ 3D NSC Xeno-Free Complete Growth Kit (RC01-CGK2)

• 1 x 10 mL VitroGel® NEURON, the first-of-its-kind synthetic biofunctional hydrogel for NSC and neuronal cultures​
• 1 x 5 mL, RocketCell™ 3D NSC Xeno-Free Suspension Medium​
• 1 x 10 µL, RocketCell™ Cell Viability Enhance (1000X)
• 1 x 500 mL, RocketCell™ NSC Xeno-Free Basal Medium​
• 1 x 10 mL, RocketCell™ NSC Xeno-Free Supplement (50X)​
• 1 x 10 µg CytoGrow™ Recombinant EGF (Human), CHO, Tag Free
• 1 x 10 µg, CytoGrow™ Recombinant bFGF/FGF-2 (Human), E. coli, Tag Free

We offer variations of the NSC Kit to fit your workflow:

• RocketCell™ NSC Xeno-Free Complete Growth Kit↗ 
  An all-in-one, ready-to-use kit for 2D neural stem cell culture.
• RocketCell™ NSC Xeno-Free Growth Medium↗ 
  Cell culture medium for NSC culture

Specifications

Use	3D proliferation and maintenance of neural stem cells
Formulation	Xeno-Free, biofunctional hydrogel and complete growth media
Injectability	Injectable hydrogel for in vivo studies and lab automation​
Operation	Ready-to-use at room temperature
No. of Use	10 mL kit 6-well, 24-well, 96-well: 2 plates
Cell Harvesting	VitroGel® Organoid Recovery Solution​ 5-15 min cell recovery
Shipping and Storage	Hydrogel: Ships at ambient temperature. Store at 2-8°C. Basal Medium: Ships with ice pack. Store at 2-8°C. Supplement Dry Ice shipping. Store at -20°C Growth Factors: Ships with ice pack/dry ice. Store at -20- -80°C.

3D NEURON CULTURE 

Figure 1. 3D Encapsulation Culture of NSC cells as a Single Cell Suspension in VitroGel® NEURON​

VitroGel® NEURON Hydrogel and RocketCell™ NSC Xeno-Free Media support 3D NSC cultures and neurite outgrowth. (A) 3D NSC cultures were established by preparing a single-cell NSC suspension with RocketCell™ NSC Xeno-Free Medium and mixing with VitroGel® NEURON for 6 days. (B) Immunofluorescence staining was performed to evaluate the presence of beta-III-tubulin, a neuron maturation marker. The images were obtained with the Keyence BZ-X microscope at 10 and 20X magnifications.

Figure 2. RocketCell™ NSC Xeno-Free Complete Growth Kit supports the expansion of NSC spheroids for differentiation with the RocketCell™ NSC-Neuron Differentiation Kit​.

iPSCs were 3D cultured in RocketCell™ 3D Xeno-Free IPSC Xeno-Free Growth Kit and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Xeno-Free Complete Growth Kit. The cells were then differentiated into neurons with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The samples were fixed and stained to evaluate the expression of (A) Microtubule-associated protein 2 (MAP2; green), (B) Neurofilament-H (red), and (C) Synaptophysin (purple). The nuclei were stained with Hoescht dye.

A

B

Figure 3. VitroGel® NEURON hydrogel supports the 3D development of dopaminergic neurons.​

iPSCs were 3D cultured in RocketCell™ 3D Xeno-Free IPSC Xeno-Free Growth Kit for 6 passages and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Xeno-Free Complete Growth Kit (A, Day 0). To induce NSC differentiation into dopaminergic neurons, the media were replaced with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The cells were exposed to the medium for 16 days (A, Day 16). The samples were fixed and stained with beta-III-tubulin and DAPI (B). It is apparent that most spheroids are neurons, and axonal connections are clearly visible at higher magnification. ​

Figure 4.  3D encapsulation culture of NSCs in VitroGel® NEURON hydrogel as NSC spheroids with zonal projections.

iPSCs were cultured and expanded using the RocketCell™ 3D iPSC Xeno-free Complete Growth kit. The cells were harvested from the hydrogel with the VitroGel® Organoid Recovery Solution and embedded in VitroGel® NEURON to induce iPSC differentiation into NSCs with CytoGrow™ Growth factors. The NSCs were differentiated into dopaminergic neurons with the RocketCell™ NSC Neuron differentiation kit.

Resources

Poster
Development of In Vitro Developing a Biofunctional Xeno-free Hydrogel Platform for Long-term 3D Neuronal Differentiation and Maintenance

Poster
The Growth and Neuronal Differentiation of Human Induced Pluripotent Stem Cells in Xeno-Free Functional Synthetic Hydrogel​