Ready-to-use, All-in-one Kit
100% synthetic kit with optimal combination of matrix and medium for 2D and 3D NSC culture and maintenance.
RocketCell™ NSC Xeno-Free Complete Growth Kit
All-in-one, ready-to-use kit for 3D and 2D neural stem cell culture
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RocketCell™ NSC Xeno-Free Complete Growth Kit
All-in-one, ready-to-use kit for 3D and 2D neural stem cell culture
Supports 3D NSC Network Formation
Promotes 3D neurite and axonal outgrowth for 3D networking formation, ideal for advanced studies.
Enhanced Cell Adhesion with Advanced Hydrogel
4X more usage than animal-based ECM for a robust, simple coating for cell adhesion
Long-Term Culture and Maintenance
Culture NSCs for over 4 weeks without compromising their multipotency.
Simple 3D and 2D Workflows at Room Temperature
Room temperature operation with simple mixing or coating steps; supports lab automation.
Easy Cell Harvesting
Simple and efficient cell harvesting with VitroGel® Organoid Recovery Solution
The RocketCell™ NSC Xeno-Free Complete Growth Kit is an all-in-one, ready-to-use kit designed to support the expansion and maintenance of neural stem cells (NSCs) in both 3D and 2D formats. This kit offers a fully defined, optimized combination of a synthetic hydrogel and a xeno-free complete medium, eliminating variability and ensuring consistent, reproducible results.
The RocketCell™ NSC Xeno-Free Complete Growth Kit provides a robust, scalable platform for long-term culture and large-scale expansion of undifferentiated NSCs in both 3D and 2D formats—while maintaining their multipotency and functional integrity. Designed for convenience and performance, this ready-to-use system operates at room temperature, integrates seamlessly with high-resolution imaging and high-throughput screening workflows for advanced neuroscience and regenerative medicine research.
The all-in-one kit includes:
2D Cell Culture:
RocketCell™ NSC Xeno-Free Complete Growth Kit (RC01-CGK1)
- 1 x 2 mL VitroGel® NEURON, the first-of-its-kind synthetic biofunctional hydrogel for NSC and neuronal cultures
- 1 x 500 mL, RocketCell™ NSC Xeno-Free Basal Medium
- 1 x 10 mL, RocketCell™ NSC Xeno-Free Supplement (50X)
- 1 x 10 µg CytoGrow™ Recombinant EGF (Human), CHO, Tag Free
- 1 x 10 µg, CytoGrow™ Recombinant bFGF/FGF-2 (Human), E. coli, Tag Free
For 3D Cell Culture:
RocketCell™ NSC Xeno-Free Complete Growth Kit (RC01-CGK2)
- 1 x 10 mL VitroGel® NEURON, the first-of-its-kind synthetic biofunctional hydrogel for NSC and neuronal cultures
- 1 x 500 mL, RocketCell™ NSC Xeno-Free Basal Medium
- 1 x 10 mL, RocketCell™ NSC Xeno-Free Supplement (50X)
- 1 x 10 µg CytoGrow™ Recombinant EGF (Human), CHO, Tag Free
- 1 x 10 µg, CytoGrow™ Recombinant bFGF/FGF-2 (Human), E. coli, Tag Free
Specifications
| Use | 3D and 2D proliferation and maintenance of neural stem cells |
| Formulation | Xeno-Free, biofunctional hydrogel and complete growth media |
| Injectability | Injectable hydrogel for in vivo studies and lab automation |
| Operation | Ready-to-use at room temperature |
| No. of Use | 2 mL kit, we recommend doing a ratio of 1:100-1:200 dilutions. 6-well: 30-60 plates 24-well plate: 25-50 plates 10 mL kit 6-well, 24-well, 96-well: 2 plates |
| Cell Harvesting | VitroGel® Organoid Recovery Solution 5-15 min cell recovery |
| Shipping and Storage | Hydrogel: Ships at ambient temperature. Store at 2-8°C. Basal Medium: Ships at ambient temperature. Store at 2-8°C for 6 months. Supplement Dry Ice shipping. Store at -20- -80°C for 1 year. Growth Factors: Store at -20- -80°C for 1 year. |
Protocols and Resources
RocketCell™ NSC Xeno-Free Growth Complete Kit – Protocol
Product Documentation
RocketCell™ NSC Xeno-Free Complete Growth Kit – Sale Sheet
Product Data Sheet
Material Safety Data Sheet (MSDS)
Data and References
2D THIN COATING METHOD
2D: CASE 1
2D NSC Maintenance (1:200 coating on VitroPrime™ Spread-Attach Plate)
A
B
Figure 1. VitroGel® NEURON 2D Thin Coating for Growth of iPSC-Derived NSCs and Confluence Analysis on VitroPrime™ Spread-Attach Plates.
A. iPSC-derived NSCs (CD34-eIPSC-NSC) were plated at 50,000 cells per well on 24-well VitroPrime™ Spread-Attach Plates coated with either VitroGel® NEURON (1:200) or Geltrex®, and cultured for 6 days. Images were captured using the Incucyte® S3 system. B. NSC growth was quantified by percent confluence over time, with images collected every 8 hours and analyzed relative to the initial timepoint (T = 0 + 4 hrs).
2D: CASE 2
2D NSC Maintenance and Characterization
(1:200 coating on VitroPrime™ Spread-Attach Plate)
Figure 2. NSC Characterization after Subculturing with the RocketCell™ NSC Xeno-Free Complete Growth Kit.
iPSC-derived NSCs (CD34-eIPSC-NSC) were plated at 50,000 cells per well on 24-well VitroPrime™ Spread-Attach Plates coated with VitroGel® NEURON (1:200). Immunofluorescence staining depicts the presence of cells positive for Nestin (A, green) and Sox2 (B, red), which are NSC markers. The nuclei were stained with DAPI (A, blue) or Hoescht (B, blue).
3D NEURON CULTURE
3D: CASE 1
3D NSC Culture with VitroGel® NEURON as Single Cell Encapsulation
Figure 3. 3D Encapsulation Culture of NSC cells as a Single Cell Suspension in VitroGel® NEURON
VitroGel® NEURON Hydrogel and RocketCell™ NSC Xeno-Free Media support 3D NSC cultures and neurite outgrowth. (A) 3D NSC cultures were established by preparing a single-cell NSC suspension with RocketCell™ NSC Xeno-Free Medium and mixing with VitroGel® NEURON for 6 days. (B) Immunofluorescence staining was performed to evaluate the presence of beta-III-tubulin, a neuron maturation marker. The images were obtained with the Keyence BZ-X microscope at 10 and 20X magnifications.
3D: CASE 2
3D Characterization of Differentiated NSC Spheroids
Figure 4. RocketCell™ NSC Xeno-Free Complete Growth Kit supports the expansion of NSC spheroids for differentiation with the RocketCell™ NSC-Neuron Differentiation Kit.
iPSCs were 3D cultured in RocketCell™ 3D Xeno-Free IPSC Xeno-Free Growth Kit and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Xeno-Free Complete Growth Kit. The cells were then differentiated into neurons with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The samples were fixed and stained to evaluate the expression of (A) Microtubule-associated protein 2 (MAP2; green), (B) Neurofilament-H (red), and (C) Synaptophysin (purple). The nuclei were stained with Hoescht dye.
3D: CASE 3
3D Development of Dopaminergic Neurons from NSC spheroids
A
B
Figure 5. VitroGel® NEURON hydrogel supports the 3D development of dopaminergic neurons.
iPSCs were 3D cultured in RocketCell™ 3D Xeno-Free IPSC Xeno-Free Growth Kit for 6 passages and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Xeno-Free Complete Growth Kit (A, Day 0). To induce NSC differentiation into dopaminergic neurons, the media were replaced with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The cells were exposed to the medium for 16 days (A, Day 16). The samples were fixed and stained with beta-III-tubulin and DAPI (B). It is apparent that most spheroids are neurons, and axonal connections are clearly visible at higher magnification.
| Size | 2 mL, 10 mL |
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Data and References
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Data and References
