Product Documentation

Sales Sheet -VitroPrime™ 3D Culture and Imaging Plates
Frequently Asked Questions

Product Data Sheet – VitroPrime™ 3D Culture and Imaging Plate – 6-well
Product Data Sheet – VitroPrime™ 3D Culture and Imaging Plate – 24-well
Product Data Sheet – VitroPrime™ 3D Culture and Imaging Plate – 96-well

Figure 1. Imaging of RFP-labeled human iPSCs (HFF-1VL-RFP, TheWell Bioscience) grown in RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit. ​

iPSCs (100k/well) from 2D cultures were plated in 24-well, VitroPrime™ 3D Culture and  Imaging Plates. After 7 days, the dishes were imaged on the Keyence BZX system (A, B) or on the Leica MICA (C, D). The Keyence system uses widefield illumination with a 1D sectioning filter to aid 3D imaging and allows 3D imaging of the IPSC spheroids in both brightfield (A) and fluorescence (B). In contrast, the MICA uses a traditional laser to capture in higher detail and render 3D models. On image D, the depth captured is 500 microns, within a field approximately 800 x 900 microns.​

Figure 2: Live Cell Surface Immunofluorescent Staining of Human IPSCs (HFF-1VL, TheWell Bioscience) Grown in RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit. ​

IPSCs (100k/well) from 2D sources were plated in 24-well VitroPrime™ 3D Culture and Imaging Plates. After 7 days, the medium was removed and replaced with media containing a diluted anti-EPCAM-PE-Alexa594-labeled antibody. The mixture was incubated for 1 hour at 37 °C, and then the well was rinsed three times with 5 min incubation with 0.5 mL of Growth Media. The well was imaged on a Leica MICA confocal microscope (A). The rendered image (B) is 300 microns deep, with a field approximately 800 x 900 microns.​

Figure 3: Vital Dyes for Monitoring the Health of IPSC (HFF-1VL-RFP, TheWell Bioscience) Grown in RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit. ​

IPSCs (100k/well) from 2D sources were plated in 24-well VitroPrime™ 3D Culture and Imaging Plates. After 7 days, the medium was removed and replaced with media containing diluted Calcein AM and DAPI. After 30 min, the dyes were removed, the well was washed with growth media, and placed back into the incubator for an additional 30 min before imaging on a Leica MICA confocal microscope (A,B). The rendered image (B) is 400 microns in depth, with a tiled field of approximately 1.4 mm x 1.1 mm.​