Premium 3D cell culture plate with unique design and premium cover-glass bottom for zero-disruption 3D cell culture workflow.
Redefining the 3D Cell Culture Workflow (In-Plate Workflow)
VitroPrime™ 3D Culture & Imaging Plates are premium-tier vessels engineered to streamline end-to-end workflows for hydrogel-based 3D culture. By integrating every step—from cell embedding and matrix polymerization to long-term culture, downstream immunofluorescence (IF) staining, and high-resolution imaging—into a single high-performance platform, VitroPrime™ minimizes sample loss and ensures consistent, reproducible results.
Mechanical Anchoring for Precision Tracking
Unlike standard plates prone to gel detachment, the VitroPrime™ 3D Culture & Imaging Plate features a unique built-in retention compartment that mechanically anchors the gel. This breakthrough design eliminates common issues such as gel floating and rotation, ensuring organoids and 3D models remain in a fixed spatial position. The resulting structural stability is critical for advanced 3D biology—enabling high-precision live-cell imaging and long-term cell tracking that are not only possible, but highly reproducible.
Zero-Disruption Automation & Medium Exchange
Each well features a dedicated lateral medium-exchange channel designed to protect the delicate 3D microenvironment. This separate port allows nutrients and reagents to diffuse smoothly and passively, without applying direct pressure to the hydrogel or disturbing the embedded 3D structures. Optimized for full lab automation, this design enables high-throughput liquid-handling systems to perform frequent media changes, fixation, and staining protocols at scale—eliminating the risk of aspirating or damaging the 3D matrix.
Elite Optical Performance
The VitroPrime™ 3D Culture and Imaging Plate features an ultra-clear #1.5 premium cover-glass bottom, engineered for researchers who demand the highest level of imaging detail. This high-performance glass maximizes light transmission while minimizing auto-fluorescence, delivering exceptional signal-to-noise ratios and diffraction-limited resolution. Ideal for confocal microscopy, high-magnification immersion objectives, and high-content screening (HCS) platforms.
| Material | Polstyrene molding and #1.5 (0.17 mm) Ultra-clear glass bottom |
| Packaging | |
| Well Plate Type | 6-well, 24-well, 96-well |
| Well Profile | • Flat bottom • Optimized compartment for hydrogel retention • Dedicated lateral medium-exchange channel |
| Sterile | Yes |
| Other Data | DNase/RNase-free, non-pyrogenic |
Sales Sheet -VitroPrime™ i3D Culture and Imaging Plates
Frequently Asked Questions
Product Data Sheet – VitroPrime™ i3D Culture and Imaging Plate – 6-well
Product Data Sheet – VitroPrime™ i3D Culture and Imaging Plate – 24-well
Product Data Sheet – VitroPrime™ i3D Culture and Imaging Plate – 96-well
Growth of mRFP-labeled IPSCs in 96-well VitroPrime™ 3D Imaging and Culture Plate.
IPSCs were harvested from 2D sources using Accutase and plated into the 96-well VitroPrime™ 3D Culture and Imaging Plate, as recommended, at a volume of 20 uL per well containing 20,000 cells/well. Cells were grown for 5 days prior to imaging using a Keyence BZX imaging system with z-stacking through the entire 1.5 mm hydrogel thickness. The brightfield (transmitted) and mRFP (reflected) images were compiled using a full-focus algorithm and displayed along the Z-axis to help visualize the shape of the light used in both imaging techniques. This data shows that cells under the angled black retaining rim thrive and grow as if in the center of the well.
VitroPrime™ 3D Culture and Imaging Plate prevents gel floating and rotation of liver organoids.
(A-E) Images indicate liver organoid growth over 30 days at the same location, cultured in VitroGel ORGANOID in a 24-well VitroPrime™ 3D Culture and Imaging Plate.
Cyto3D® Live-Dead Assay for Monitoring the Health of IPSC (HFF-1VL, TheWell Bioscience) Grown in RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit.
IPSCs (100k/well) from 2D sources were plated in a 24-well, VitroPrime™ 3D Culture and Imaging Plate. After 7 days, the medium was removed and replaced with media containing a 1:50 dilution of the Cyto3D® Live-Dead Assay solution. After 30 min, the well was imaged on a Leica MICA confocal microscope (A, B). The rendered image (B) is 600 microns deep, with a tiled field approximately 1.4 mm x 1.1 mm. The data also demonstrate that the Cyto3D® protocol does not require any wash steps and can expedite imaging protocols used to assess the vitality of live cultures.
Live Cell Surface Immunofluorescent Staining of Human IPSCs (HFF-1VL, TheWell Bioscience) Grown in RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit.
IPSCs (100k/well) from 2D sources were plated in a 24-well VitroPrime™ 3D Culture and Imaging Plate. After 7 days, the medium was removed and replaced with media containing a diluted anti-EPCAM-PE-Alexa594-labeled antibody. The mixture was incubated for 1 hour at 37 °C, and then the well was rinsed three times with 5 min incubation with 0.5 mL of Growth Media. The well was imaged on a Leica MICA confocal microscope (A). The rendered image (B) is 300 microns deep, with a field approximately 800 x 900 microns.
Direct Immunofluorescence Staining of IPSCs (HFF-1VL, TheWell Bioscience) Grown in RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit.
iPSCs (100k/well) were grown for 7 days in a 24-well VitroPrime™ 3D Culture and Imaging Plate before fixation and staining with directly labeled pluripotency markers Lin28 (Alexa 488), Podocalyxin (Alexa 594), and Nanog (Alexa 647). Images were captured using a Leica MICA confocal microscope. These results show that the RocketCell™ 3D iPSC Xeno-Free Complete Growth Kit provides a supportive 3D environment for maintaining pluripotent stem cells, while the use of directly labeled antibodies enables faster and more efficient hydrogel-based sample processing.
iPSC-derived intestinal organoids cultured and stained in VitroPrime™ 3D Culture and Imaging Plate.
Representative images showing iPSC-derived intestinal organoids cultured in VitroGel® ORGANOID. iPSC cells were cultured in VitroGel® STEM for the generation of spheroids. The spheroids were then differentiated into intestinal organoids using CytoGrow™ growth factors. Once matured organoids were fixed and stained without disturbing cultures in the VitroPrime™ i3D Cell Culture plate. (A) Blue staining (DAPI) represents cell nuclei (B). Yellow fluorescence: Phalloidin (C), Magenta: Villin protein. (D) Merged image.
| Plate Size | 6-well, 24-well, 96-well |
|---|---|
| Pack Size | 5 Plates/Pack |
Culture Plates
Unique surface treated for superior hydrogel spreading, adherence, and uniform surface.
Culture Plates
Premium U-bottom cell culture plate for 3D spheroids.
3D Culture Vessels
Transparent, PET, Sterile, 48 inserts (12/PK, 4PK/CS)
