Complete, Fully Defined Medium System
A xeno-free, chemically defined formulation combining optimized supplements with a robust basal medium for seamless hMSC expansion.
New
RocketCell™ hMSC Xeno-Free Complete Growth Medium
High-performance, chemically-defined kit for superior expansion of human mesenchymal stem cells (hMSC)
RocketCell™ hMSC Xeno-Free Complete Growth Medium
High-performance, chemically-defined kit for superior expansion of human mesenchymal stem cells (hMSC)
Optimized ECM Production
The formulation promotes endogenous extracellular matrix secretion, as evidenced by a gel-like matrix in high-density cultures, thereby enhancing cell attachment and microenvironment stability.
High Reproducibility and Regulatory Readiness
Ensures high reproducibility, long-term stability, and regulatory compliance for advanced research and translational use.
Enhanced Cell Survival and Proliferation
Supports low-density seeding, maintains high post-passaging viability, and promotes consistent culture renewal.
Weekend-Free Workflow
An alternate-day feeding schedule minimizes handling and supports a consistent, long-term culture.
Preserved Multipotency
Maintains classical tri-lineage differentiation capacity into adipogenic, chondrogenic, and osteogenic lineages after extended expansion.
RocketCell™ hMSC Xeno-Free Complete Growth Medium is a fully defined, animal component–free formulation designed for robust, rapid, and reproducible expansion of human mesenchymal stem cells (hMSCs). This complete medium system integrates an optimized basal medium with a chemically defined supplement to ensure high stability and potency for consistent, long-term performance.
The kit has been rigorously validated with multiple mesenchymal stem cell lines, producing uniform cultures with key multipotency markers, including CD73, CD90, and CD105. For smooth transition from serum-based media, initial culture on fibronectin-coated plates is recommended. After short-term adaptation, cells can be expanded on standard cultureware without additional coating.
Developed to address the challenges of conventional MSC culture—including batch variability, undefined components, poor low-density expansion, and loss of multipotency—RocketCell™ hMSC Xeno-Free Complete Growth Medium enables reliable cell growth for advanced research, translational development, and cell therapy manufacturing.
Kit Contents:
- 1 x 485 mL, RocketCell™ hMSC Xeno-Free Basal Medium
- 1 x 15 mL, RocketCell™ hMSC Xeno-Free Supplement
Specifications
| Formulation | Xeno-free complete culture medium kit |
| Use | Expansion of hMSCs |
| pH | Neutral |
| Cell Harvesting | Enzymatic (TrypLE or similar recommended) |
| Shipping and Storage | Supplement: Ships with dry ice. Store at -20°C. Basal Medium: Ships with ice pack. Store at 2-8°C. |
Challenges We Solve
Key Challenges in hMSC Culture
Inconsistent performance & undefined materials
Animal-derived or poorly defined components introduce variability and compromise reproducibility.
Loss of Potency
Spontaneous differentiation may occur when using
animal-based ECM.
Poor Expansion
Traditional systems have low seeding density and high media consumption, limiting expansion.
Labor-intensive culture workflow
Frequent feedings and weekend maintenance limit flexibility and throughput.
RocketCell™ hMSC Advantages
Xeno-free and Chemically-defined composition
100% xeno-free composition removes the risks and variability. Ensures batch-to-batch consistency and long-term stability.
Maintains Multipotency
Specifically formulated to prevent spontaneous differentiation and maintain multipotency.
High 3D Culture Capability
Optimized to ensure high proliferation rates, even at low seeding densities.
Weekend-free Workflow
Allows for an alternate-day feeding schedule, simplifying lab operations.
Protocols and Resources
RocketCell™ hMSC Xeno-Free Growth Medium – Protocol
Product Documentation
RocketCell™ hMSC Xeno-Free Growth Medium – Sale Sheet
Product Data Sheet
Data and References
Weekend-Free Workflow
MSC recovery and expansion workflow with RocketCell™ hMSC Xeno-Free Complete Growth Medium
CASE 1
Growth of hMSCs Cryopreserved for 20 years in
RocketCell™ hMSC Xeno-Free Complete Growth Medium.
Figure 1: Growth of Cryopreserved hMSCs in RocketCell™ hMSC Xeno-Free Complete Growth Medium.
An ampoule of hMSCs was defrosted after 20 years of storage in liquid nitrogen vapor. Cells were plated into a 24-well fibronectin-coated VitroPrime™ Spread Attach plate and placed into a low O2 incubator (6% O2). The media was changed after 48h, and at 72h, the plate was placed in an IncuCyte S3 and scanned every 4 hours to track cell growth.
CASE 2
Growth of hMSCs Post TrypLE Passage in
RocketCell™ hMSC Xeno-Free Complete Growth Medium.
Figure 2: Growth of TrypLe Passaged hMSCs in RocketCell™ hMSC Xeno-Free Complete Growth Medium:
hMSC cultures grown in a Fibronectin-coated T75 flask were passaged using TrypLe and plated onto a Fibronectin-coated 24-well VitroPrime™ Spread-Attach Plate at a density of 10,000 cells/well (~5000 cells/cm2). The plate was immediately placed into an Incucyte S3 and imaged over thecourse of 163h, with media changed every 48 hours.
CASE 3
Growth of hMSCs Post TrypLE Passage in RocketCell™ hMSC Xeno-Free Complete Growth Medium on Uncoated, Vitronectin and Fibronectin-coated VitroPrime™ Spread Attach Plate
Figure 3: Growth of TrypLe Passaged hMSCs in RocketCell™ hMSC Xeno-Free Complete Growth Medium on Different ECMs.
hMSC cultures were passaged from T75 Fibronectin-coated flask and plated on
uncoated, Vitronectin (10 ug/mL) or Fibronectin (10 ug/mL) coated 12-well VitroPrime™ Spread-Attach Plate. The cells were incubated for 72 hours, then imaged on an Incucyte S3.
CASE 4
Immunostaining of hMSCs Cultured in RocketCell™ hMSC Xeno-Free Complete Growth Medium
Figure 4: Immunostaining of hMSCs in RocketCell™ hMSC Xeno-Free Complete Growth Medium.
hMSC cultures were plated on Fibronectin (10 ug/mL) coated 24-well VitroPrime™ Spread Attach Plate. After 6 days, the cells were processed for standard immunofluorescence and stained with directly labeled antibodies: CD90-FITC, CD105-PE, and CD73-APC. The staining was observed and documented using a Leica MICA confocal microscope.
CASE 5
Tri-Lineage Differentiation of hMSCs Cultured in
RocketCell™ hMSC Xeno-Free Complete Growth Medium
Figure 5. Tri-Lineage Differentiation of hMSCs cultured in RocketCell™ hMSC Xeno-Free Complete Growth Medium.
hMSC cultures were plated on a 24-well Fibronectin (10 ug/mL)- coated VitroPrime™ Spread Attach Plate. After 6 days of expansion in RocketCell™ hMSC Xeno-Free Complete Growth Medium, the cells were placed into Adipogenic, Chondrogenic, and Osteogenic Media for 12 days. The cultures were fixed and processed for standard chromogenic staining with Oil Red O for adipocytes, Alcian Blue for chondrocytes/cartilage, and Alizarin Red for Osteocytes. The staining was observed and documented using a Keyence BZX microscope.
Enhance your hMSC culture with these products:
CytoGrow™ - Recombinant Proteins
Premium CytoGrow™ growth factor suitable for cell culture coating, ECM research, wound healing, angiogenesis, tissue engineering, and regenerative medicine.
CytoGrow™ - Recombinant Proteins
Premium CytoGrow™ growth factor suitable for cell culture coating, extracellular matrix research, wound healing, angiogenesis, tissue engineering, and regenerative medicine.
3D Culture Vessels
Unique surface treated for superior hydrogel spreading, adherence, and uniform surface.
Hydrogels - Ready-To-Use
ready-to-use, xeno-free (animal origin-free) hydrogel system for mesenchymal stem cell 3D culture and scale-up
| Kit | 1 Kit |
|---|
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