Figure 1. NSC Characterization after Subculturing with the RocketCell™ NSC Xeno-Free Growth Medium.

iPSC-derived NSCs (CD34-eIPSC-NSC) were plated at 50,000 cells per well on 24-well VitroPrime™ Spread-Attach Plates coated with VitroGel® NEURON (1:200). Immunofluorescence staining depicts the presence of cells positive for Nestin (A, green) and Sox2 (B, red), which are NSC markers. The nuclei were stained with DAPI (A, blue) or Hoescht (B, blue).

Figure 2. RocketCell™ NSC Xeno-Free Growth Medium supports the expansion of NSC spheroids for differentiation.

iPSCs were 3D cultured in RocketCell™ 3D Xeno-Free IPSC Xeno-Free Growth Kit and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Xeno-Free Growth Medium. The cells were then differentiated into neurons with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The samples were fixed and stained to evaluate the expression of (A) Microtubule-associated protein 2 (MAP2; green), (B) Neurofilament-H (red), and (C) Synaptophysin (purple). The nuclei were stained with Hoescht dye.