Protocols and Resources
RocketCell™ Organoid Xeno-Free Essential-Core Medium – Protocol
Product Documentation
RocketCell™ Organoid Xeno-Free Essential-Core Medium – Sale Sheet
Product Data Sheet
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RocketCell™ Organoid Xeno-Free Essential-Core Medium
Advanced xeno-free essential core medium for organoid development that eliminates the need for supplemental additives such as N2 and B27.
Available on backorder
$329.00
RocketCell™ Organoid Xeno-Free Essential-Core Medium
The complete xen-free foundation for organoid culture – just add growth factors.
The RocketCell™ Organoid Xeno-Free Essential-Core Medium provides a complete foundation for organoid culture, including essential supplements for organoid development. This advanced essential core medium can replace the separate addition of supplements such as N2 and B27 in organoid culture medium. Depending on the organoid type, RocketCell™ Organoid Xeno-Free Essential-Core Medium should be supplemented with growth factors for optimum growth. This is a 100% Xeno-free environment (animal origin-free).
The RocketCell™ Organoid Xeno-Free Essential-Core Medium is optimized and includes essential supplements and other small molecules (excluding growth factors) that provide the nutritional support necessary for long-term organoid growth. RocketCell™ Organoid Xeno-Free Essential-Core Medium Kit is recommended for organoid development in both synthetic and animal-based hydrogels.
Kit Contents:
- 10 mL, RocketCell™ Organoid Xeno-Free Essential-Core Supplement (50X)
- 490 mL, RocketCell™ Organoid Xeno-Free Essential-Core Basal Medium
Specifications
| Use | For Organoid Cultures |
| Cell Harvesting | No enzymatic or enzymatic (VitroGel® Organoid Recovery Solution, TrypLE or similar recommended) |
| Shipping | Requires ice pack • RocketCellTM Organoid Xeno-Free Essential-Core Basal Medium (500 mL) Requires dry ice shipping • RocketCellTM Organoid Xeno-Free Essential-Core Supplement (50X) (10 mL) |
| Storage | Store at 2-8°C • RocketCellTM Organoid Xeno-Free Essential-Core Basal Medium (500 mL) Store at -20°C • RocketCellTM Organoid Xeno-Free Essential-Core Supplement (50X) (10 mL) |
Data and References
iPSC-derived intestinal organoids cultured and stained in VitroPrime™ 3D Culture and Imaging Plate.
The entire xeno-free workflow—from cell culture to imaging—was performed directly on the VitroPrime™ 3D Culture and Imaging Plate, without removing cells or organoids from the wells. iPSCs were initially cultured in VitroGel® STEM to form spheroids and subsequently differentiated into intestinal organoids using CytoGrow™ growth factors. RocketCell™ Organoid Xeno-Free Essential-Core Medium was used to ensure optimal organoid growth. Matured intestinal organoid was fixed, stained, and imaged using a Leica Mica confocal microscope. (A) DAPI staining (blue) indicates cell nuclei; (B) Phalloidin staining (yellow); (C) Villin protein staining (magenta); and (D) merged image.
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