Get 20% OFF your Cyto3D® Live-Dead Assay Kit

Experience what other researchers are uncovering: a Live/Dead Assay Kit that brings unmatched speed, simplicity, and clarity to 3D cell culture viability assessment.

To activate this promo:

• ORDER ONLINE and use promotional code CYTO3D20 in the cart before checkout.

Terms and Conditions:
The promotion is valid for orders placed directly with TheWell Bioscience and shipped to a U.S. address. Not valid through distributors.  Some exclusions may apply.  We reserve the right to modify or cancel this offer at any time. Promo runs until December 3, 2025, or while supplies last.

Discover just how easy, fast, and simple 3D live–dead assays can be with the following resources. Learn how researchers are streamlining viability analysis in 3D cell culture using Cyto3D® Live-Dead Assay Kit—a fast,  one-step staining procedure with no pre-mixing required.

A Quick Organoid Viability Measurement by Using Cyto3D® Live-Dead Assay Kit

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Need more proof? Explore our growing list of publications that show why researchers worldwide trust Cyto3D® as the leading and preferred live–dead assay kit for 3D cell culture.

• Imamura, H., Tomimaru, Y., Akita, H., Ito, T., Mukai, Y., Sasaki, K., Hasegawa, S., Yamada, D., Noda, T., Takahashi, H., Kobayashi, S., Doki, Y., & Eguchi, H. (2025). Graft trimming with LigaSureTM and leak testing with Indigo carmine reduces blood loss significantly after reperfusion in pancreas transplantation. Surgery Today. https://doi.org/10.1007/s00595-025-03173-0
• Raza, A., Imran, M., Škalko-Basnet, N., & Obuobi, S. (2025). Tannic acid-controlled crosslinking of albumin amyloids as multifunctional hydrogels against chronic wounds. International Journal of Biological Macromolecules, 148707. https://doi.org/10.1016/j.ijbiomac.2025.148707
• Sakamoto, T., Koma, H., Ayane Kuwano, Horie, T., Atsushi Fuku, Kitajima, H., Nakamura, Y., Ikuhiro Tanida, Yujiro Nakade, Yoshiyuki Tachi, Ikki Horiguchi, Yamamoto, N., Yamada, S., Guo, X., Yang, Q., Yasuhito Ishigaki, Toru Ichiseki, Ayumi Kaneuji, Osawa, S., & Kawahara, N. (2025). Methylcellulose in culture medium regulates size, cell viability, cytokine production, and exosome secretion in adipose-derived stem cell spheroids cultured using SphereRing® system. Biochemical Engineering Journal, 109929–109929. https://doi.org/10.1016/j.bej.2025.109929
• Nightheart, S., Rauff, A., Dinh, E., Burger, A., Jones, K. A., Romanowicz, G. E., Leguineche, K., Lin, A. S. P., & Guldberg, R. E. (2025). Oxygenation and Temperature Conditioning Alter the Vascular Morphology of Microvascular Constructs. Tissue Engineering. Part A, 10.1177/19373341251364552. https://doi.org/10.1177/19373341251364552
• Park, J., Kim, D., Park, H.-C., Park, M., Zhang, S., Okcho Na, Lee, Y. S., Lee, M., Ahn, S., & Chung, Y.-J. (2025). Tumor microenvironment-preserving gliosarcoma organoids as an in vitro preclinical platform: a comparative analysis with glioblastoma models. Journal of Translational Medicine, 23(1). https://doi.org/10.1186/s12967-025-06952-y
• Srivastava, A., Bencomo, T., Lee, C.-N., Mah, A., Garcia, J., Seow, L. W., Donohue, I. M., Tan, A. J., Nguyen, A., Jiang, T., Gombar, S., Phu, L., Dwivedi, P., Rose, C. M., Brown, R., & Lee, C. S. (2025). Epithelial tumor cells utilize mast cell-derived histamine to regulate perineural invasion. BioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2025.06.23.661147
• Raza, A., Nataša Škalko-Basnet, & Obuobi, S. (2025). Alginate/Polyethylene glycol diacrylate shape memory hydrogel films for gastric retention and antibiotic delivery in H. pylori infection. Carbohydrate Polymer Technologies and Applications, 100967–100967. https://doi.org/10.1016/j.carpta.2025.100967
• Motoike, S., Inada, Y., Toguchida, J., Kajiya, M., & Ikeya, M. (2025). Jawbone-like organoids generated from human pluripotent stem cells. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-025-01419-3
• Lee, H. J., Lau, L. N., Sidhu, S. K., Park, J.-Y., & Yeo, I.-S. L. (2025). Three-Dimensional Hydrogel Culture Reveals Novel Differentiation Potential of Human Bone Marrow-Derived Stem Cells. Prosthesis, 7(3), 52. https://doi.org/10.3390/prosthesis7030052
• Díaz Méndez, A. B., Di Giuliani, M., Sacconi, A., Tremante, E., Lulli, V., Di Martile, M., Vari, G., De Bacco, F., Boccaccio, C., Regazzo, G., & Rizzo, M. G. (2025). Androgen receptor inhibition sensitizes glioblastoma stem cells to temozolomide by the miR-1/miR-26a-1/miR-487b signature mediated WT1 and FOXA1 silencing. Cell Death Discovery, 11(1). https://doi.org/10.1038/s41420-025-02517-6
• Lee, E.-O., Joo, H. K., Yoo, H. J., Kim, C.-S., & Jeon, B. H. (2025). Aspirin-induced acetylation of APE1/Ref-1 enhances RAGE binding and promotes apoptosis in ovarian cancer cells. Korean Journal of Physiology and Pharmacology. https://doi.org/10.4196/kjpp.24.273
• DePalma, T., Rodriguez, M., Kollin, L., Hughes, K., Jones, K., Stagner, E., Venere, M., & Skardal, A. (2025). A Microfluidic Blood Brain Barrier Model to Study the Influence of Glioblastoma Tumor Cells on BBB Function. Small. https://doi.org/10.1002/smll.202411361
• Kim, M. S., & Kim, M. S. (2025). Deubiquitination of epidermal growth factor receptor by ubiquitin-specific peptidase 54 enhances drug sensitivity to gefitinib in gefitinib-resistant non-small cell lung cancer cells. PLoS ONE, 20(4), e0320668–e0320668. https://doi.org/10.1371/journal.pone.0320668
• Patel, D. P. (2025). Developing and Validating Brain Tumor Organoid Models to Evaluate Novel Therapeutics In Vitro. Scholar Commons. https://scholarcommons.sc.edu/senior_theses/741/
• Chang, W.-H., Chin, A. I., & Chen, C.-H. (2025). Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy. STAR Protocols, 6(1), 103623–103623. https://doi.org/10.1016/j.xpro.2025.103623
• Donato, M. D., Cristiani, C. M., Capone, M., Garofalo, C., Madonna, G., Passacatini, L. C., Ottaviano, M., Paolo Antonio Ascierto, Auricchio, F., Carbone, E., Migliaccio, A., & Castoria, G. (2025). Role of the androgen receptor in melanoma aggressiveness. Cell Death and Disease, 16(1). https://doi.org/10.1038/s41419-025-07350-4
• Kawai-Kawachi, A., Lenormand, M. M., Astier, C., Herbel, N., Cutrona, M. B., Ngo, C., Garrido, M., Eychenne, T., Dorvault, N., Bordelet, L., Song, F. F., Bouyakoub, R., Loktev, A., Romo-Morales, A., Henon, C., Colmet-Daage, L., Vibert, J., Drac, M., Brough, R., & Schwob, E. (2025). Replication Stress is an Actionable Genetic Vulnerability in Desmoplastic Small Round Cell Tumors. Cancer Res. https://doi.org/10.1158/0008-5472.CAN-23-3603
• Kim, K., Lee, Y., Jung, K. B., Kim, Y., Jang, E., Lee, M., Son, M., & Lee, H. J. (2024). Highly Stretchable 3D Microelectrode Array for Noninvasive Functional Evaluation of Cardiac Spheroids and Midbrain Organoids. Advanced Materials. https://doi.org/10.1002/adma.202412953
• Ossi Arasalo, Lehtonen, A. J., Kielosto, M., Heinonen, M., & Juho Pokki. (2024). Probabilistic analysis of spatial viscoelastic cues in 3D cell culture using magnetic microrheometry. Biophysical Journal. https://doi.org/10.1016/j.bpj.2024.12.010
• Motawea, A., Maria, S. N., Maria, D. N., Jablonski, M. M., & Mohamed Moustafa Ibrahim. (2024). Genistein transfersome-embedded topical delivery system for skin melanoma treatment: in vitro and ex vivo evaluations. Drug Delivery, 31(1). https://doi.org/10.1080/10717544.2024.2372277
• Meng, H., Miao, H., Zhang, Y., Chen, T., Yuan, L., Wan, Y., Jiang , Y., Zhang , L., & Cheng , W. (2024). YBX1 promotes homologous recombination and resistance to platinum-induced stress in ovarian cancer by recognizing m5C modification. Cancer Letters, 217064–217064. https://doi.org/10.1016/j.canlet.2024.217064
• Noriyuki Imazu, Takehiro Torisu, Akihito Yokote, Junji Umeno, Keisuke Kawasaki, Shin Fujioka, Yuichi Matsuno, Tomohiro Nagasue, Shinichiro Kawatoko, Tomohiko Moriyama, Tomoki Nitahata, Yushi Uchida, Seishi Aihara, Yoshiaki Taniguchi, Yoshinao Oda, & Takanari Kitazono. (2024). Arginase 2 attenuates ulcerative colitis by antioxidant effects of spermidine. Journal of Gastroenterology. https://doi.org/10.1007/s00535-024-02104-z
• Cheng, W.-M., Li, P.-C., Minh Tran-Binh Nguyen, Lin, Y.-T., Huang, Y.-T., Cheng, T.-S., Nguyen, T.-H., Tran, T.-H., Huang, T.-Y., Hoang, T.-H., Chen, S.-Y., Chu, Y.-C., Wu, C.-W., Lee, M.-F., Chiou, Y.-S., Liu, H.-S., Hong, Y.-R., Peter Mu-Hsin Chang, Hu, Y., & Chang, Y.-C. (2024). Repurposing pitavastatin and atorvastatin to overcome chemoresistance of metastatic colorectal cancer under high glucose conditions. Research Square (Research Square). https://www.researchsquare.com/article/rs-4218809/latest
• Badawe, H., Harouz, J., Raad, P., Abu, K., Freije, Anthony., Ghali, K., Abou-Kheir, W., Khraiche, M. (2024). Experimental and Computational Analysis of High-Intensity Focused Ultrasound Thermal Ablation in Breast Cancer Cells: Monolayers vs. Spheroids. Cancers 2024, 16(7), 1274; https://doi.org/10.3390/cancers16071274