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Can VitroGel® be used for both 3D culture and 2D hydrogel coating? Which culture method should I choose?
< 1 min read
Yes, VitroGel® can support 3D cell culture, 2D thick gel coating, and 2D thin gel coating culture. Embedding cells in the hydrogel matrix (3D cell culture) can maximize the cell-matrix interaction and potential cell-cell communication in response to the hydrogel’s biophysical and biological properties. Some cells are hard to culture in 3D; as a result, 2D coating is an alternative way.
2D thick gel coating uses a layer of hydrogel to change the properties of the substance. It is a method between traditional 2D culture and true 3D cell culture. 2D thick gel coating is an alternative approach if scientists do not want to jump into 3D cell culture but want to see how cells behave differently based on different substances. It is also a good method for layer-by-layer co-culture.
A 2D thin gel coating is used as a thin layer of hydrogel to change the substance’s surface properties, which may change its capability for cell attachment but not its stiffness.
