How fast should I transfer the sample from the mixing tube to the culture plate?

After mixing with the cell culture medium, we recommend transferring the mixture to the tissue culture plate immediately. The hydrogel formation starts after mixing the VitroGel solution with the cell culture medium.

If you have multiple samples with different hydrogel conditions or cell types to prepare, we recommend transferring the mixture of sample 1 to the tissue culture plate before mixing the hydrogel with the cell culture medium for sample 2.