What is the recommended number of cells for VitroGel injection?

The typical implanting cell number is between 1 to 10 x 106, depending on different cell types. The cell suspension can be prepared in PBS to obtain 2-20 x 107 cells per mL and then be mixed with VitroGel at a 1:1 ratio (v/v). The cell density can be adjusted based on the inoculation volume (e.g., 100 or 200 μL) and the mixing ratio between VitroGel:Cell suspension. Please check the user protocol for different mixing ratios.