Is it possible to dilute VitroGel hydrogels with cell culture medium instead of the dilution solution?

For “High-Concentration” tunable hydrogels, it is not recommended.  The concentration of the ionic molecule in the medium would be much higher than the dilution solution, so diluting VitroGel with the cell culture medium may cause the gel to form too quickly and produce a chunky consistency. We recommend using the VitroGel Dilution Solution to adjust the highly concentrated VitroGel.

Alternatively, the “Ready-To-Use” VitroGel hydrogels are a better option for scientists who do not want to worry about dilution and only want to mix the hydrogel with cell suspension directly.