What is the optimal ratio between cell suspension and gel for the mix?

For the Ready-To-Use VitroGel Hydrogel system (blue cap), the mixing ratio is 2:1. For our High-concentration VitroGel system (red cap), the mixing ratio is 4:1. The mixing ratio is also tunable depending on cell culture medium. For example, if the cell medium has low ionic molecule concentration, such as RPMI, we recommend a 1:1 mixing ratio for ready-to-use hydrogel.