Should I use treated or non-treated culture for dome methods?

For the dome method,

  • The non-tissue culture-treated plate works better for hydrogel to hold the dome shape (25 uL hydrogel in each well of the 24-well plate).
  • However, if you use the hydrogel the cover the whole bottom of the well plate, the tissue culture-treated plate helps the hydrogel to spread and cover the whole bottom of the well plate (sealing the edge of the bottom and wall of each well better), which can help to hold the hydrogel at the bottom of the well plate.
  • Alternatively, you could coat the plate with 0.1-0.5% gelatin for 10 minutes and then remove the gelatin before adding the hydrogel.