Recombinant bovine transforming growth factor-beta 3 (TGF-β3) is produced in CHO cells in a tag-free format, ensuring native dimeric folding and mammalian-specific post-translational modifications. TGF-β3 is a highly conserved member of the TGF-β superfamily, functioning as a potent regulator of embryogenesis, cell differentiation, and extracellular matrix (ECM) synthesis.

In bovine and cross-species models, TGF-β3 signals through the canonical SMAD pathway via the TGF-β Type I and Type II receptor complex. It is particularly recognized for its superior ability, compared to other isoforms, to induce chondrogenic differentiation and promote scarless wound healing by modulating the deposition of collagen and fibronectin. The CHO-derived, tag-free expression system ensures high specific bioactivity and eliminates potential interference with receptor binding or downstream signaling, providing a reliable and chemically defined tool for complex tissue modeling.