NEW
Clear glass vial with white cap, labeled 'RocketCell S1 Supplement (50x), AOF' for research use only, sterile
Fluorescent micrograph of a neural network: green cell bodies with magenta-colored neurites extending between them.
RocketCell™ S1 Supplement (50X), AOF - Image 3
Clear glass vial with white cap, labeled 'RocketCell S1 Supplement (50x), AOF' for research use only, sterile
Fluorescent micrograph of a neural network: green cell bodies with magenta-colored neurites extending between them.
RocketCell™ S1 Supplement (50X), AOF - Image 3

RocketCell™ S1 Supplement (50X), AOF

B27-like, Animal Origin-Free Supplement

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RocketCell™ S1 Supplement (50X), AOF

Animal Origin-Free B27-like Supplement

Comprehensive Antioxidant protection
An optimized blend of antioxidant enzymes and vitamins that helps protect sensitive cells from oxidative damage, supporting high viability even at low plating densities.

3D-Validated
Specifically optimized for use within VitroGel® hydrogel systems for spheroid and organoid applications.

Three stacked rounded rectangles in a row with a checkmark circle above, symbolizing completion or approval of all three items.

Drop-in replacement
Protocol-compatible with standard B27 workflows at equivalent working concentrations.

Retinoid-Competent Formulation
Supports baseline CNS neuronal maintenance, maturation, and long-term culture stability.

Blue line diagram showing a culture dish at the top with three small circular cells inside, and three arrows pointing to three distinct neural cell icons below (neuron-like shapes) indicating derivation or transfer from the dish.

Broad and Versatile
Supports organoid, tumoroid, hippocampal and cortical neuron cultures, iPSC-derived neuron maturation, cancer stem cell, and cardiomyocyte maintenance.

RocketCell™ S1 Supplement, AOF is an animal origin-free replacement for conventional B27 supplements, engineered for the long-term survival, maturation, and functional maintenance of CNS neurons and a broad spectrum of other cell types. All components are of recombinant, synthetic origin, eliminating the batch-to-batch variability and safety concerns inherent in animal-derived additives.

This is the all-purpose formulation — the right choice when the primary goal is maximizing long-term cell viability without directing cell fate. Supplied as a 50X liquid concentrate for use with standard neurobasal or serum-free basal media.

Recommended Products

Protocols and Resources

 Protocol

Product Documentation

Sale Sheet

 Product Data Sheet

 Material Safety Data Sheet (MSDS)

Frequently Asked Questions 

Data and References

ORGANOIDS

Cryo-Recovery and Re-Culturing Intestinal Organoid with RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF ​

Cryo-recovery and re-culturing of intestinal organoids using RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF.

Representative bright-field images show cryopreserved intestinal organoids following recovery at Day 0 (A) and subsequent growth at Day 12 (B) in VitroGel®. Quantitative analysis demonstrates progressive organoid expansion over 12 days across three replicate wells, indicating successful recovery and sustained growth following cryopreservation.​

Mouse Intestinal Organoid Cultured using RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF ​

Panel A: Yellow fluorescent cell cluster with scattered bright dots on a black background; scale bar 50 μm.
Fluorescent image showing yellow-stained cell nuclei clustered in a dark field (panel D).

Confocal fluorescence imaging of mouse intestinal organoids.

Mouse intestinal organoids were cultured using RocketCell™ S1 & S2 Supplements, AOF and stained to assess morphology and structural integrity. (A) Nuclear localization visualised by fluorescent staining (yellow). (B) F-actin/membrane visualization highlighting crypt-like budding and luminal architecture (cyan). (C) Merged overlay of nuclear (yellow) and structural (cyan) channels demonstrating organized cellular distribution. (D) Maximum intensity projection / representative z-frame highlighting nuclear density across the 3D organoid volume. Scale bars = 50 µm.

SPheroids

iPSC-derived Neuroectodermal Spheroid Culture in VitroGel® Hydrogels
and the RocketCell™ S1 (B27-like) Supplement, AOF ​

Collage comparing ViTroGel STEM (top) and NEURON (bottom): left panels show round speckled gel disks, middle panels show grayscale electron micrographs of cells, right panels show close-up of a textured circular cell structure.
Blue nuclei stained with Hoechst in a dense cell cluster; scale bar shows 200 µm; dark background.

3D iPSC-derived Floor Plate Neuroepithelium Spheroids.

iPSC spheroids cultured with the RocketCell™ 3D iPSC Complete Growth Kit, AOF were transitioned into FPNE1 Medium, and cultured for 4 days, passaged into VitroGel® STEM or VitroGel® NEURON in FPNE medium supplemented with RocketCell™ S1 Supplement, Minus Insulin, AOF and characterized after 8 days. These spheroids expressed canonical FPNE markers such as FoxA2, Netrin, and Nkx2.2.​

Floor Plate Neuron Epithelium Spheroid Differentiation into Motor Neurons with the RocketCell™ S1 (B27-like) Supplement, AOF ​

A

Three-panel microscopy image: left shows a circular field densely populated with small dark particles; center and right show two large dark clusters with a fibrous halo.

B

Fluorescent micrograph of neuron networks in purple and green with a highlighted magnified inset on the left panel and a zoomed section on the right panel, showing dense neural connections.

Floor Plate Neuron Epithelium Culture Differentiation into Motor Neurons​

​A. FPNE cultures were transitioned to FPNE3 patterning medium for 4 days and transitioned to Neuron Differentiation Medium with the RocketCell™ S1 Supplement, AOF for 14 days. ​

​B. The cultures were fixed and processed for FL immunostaining using anti-phosphorylated neurofilament (purple) and CHAT (green) for motor neurons. This system demonstrates the power of developing complex interconnected systems capable of spanning multi-millimeter volumes in 3D with thousands of interconnected neuron spheroids and interneurons.​

Supplement Type

Standard, Minus Insulin, Minus Vitamin A

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