Apical-Out Intestinal Organoid Generation
Designed to generate apical-out organoids, enabling direct access to the apical surface while maintaining stability.
New
RocketCell™ Apical-out Intestinal Organoid Xeno-Free Growth Kit
All-in-one, xeno-free kit for growth expansion and passaging of intestinal organoids.
RocketCell™ Apical-out Intestinal Organoid Xeno-Free Growth Kit
All-in-one, xeno-free kit for growth expansion and passaging of intestinal organoids with apical-out polarity.
Fully Xeno-Free and Chemically Defined System
Eliminates all animal-derived components and reduces batch-to-batch variability.
Fast and Easy-to-use
Reduces media preparation time since it is ready-to-use. User-friendly protocol to follow.
Long-term Maintenance of Apical-out Polarity
Supports long-term intestinal organoid culture while maintaining apical-out polarity.
Improves Organoid Survival and Growth
Supports initial intestinal organoid survival, maintains high post-passaging viability and consistent culture renewal.
Scalable and Automation-Friendly
Compatible with multiwell plates and high-throughput assays.
RocketCell™ Apical-Out Intestinal Organoid Growth Kit is a complete solution for generating mature apical-out intestinal organoids in a fully xeno-free environment. The kit includes three ready-to-use media formulations for initiation and maintenance, along with VitroGel® ORGANOID-5, a synthetic hydrogel.
Organoid polarity is critical for physiological relevance. Conventional systems using animal-derived matrices (e.g., Matrigel) produce apical-in organoids, limiting access to the apical surface and restricting studies of nutrient uptake, drug response, and host–microbiome interactions. Alternative methods to reverse polarity often compromise structural integrity and function. This kit is specifically designed to generate apical-out organoids, enabling direct access to the apical surface while maintaining stability.
RocketCell™ Apical Out Intestinal Organoid Xeno-Free Growth Kit
(RC03-GK)
- 1 x 10 mL, VitroGel® ORGANOID-5
- 1 x 5 mL, RocketCell™ IO XF Cell Suspension Medium
- 1 x 100 mL, RocketCell™ IO XF Growth Medium
- 1 x 50 µL, RocketCell™ Cell Viability Enhancer
RocketCell™ Apical Out Intestinal Organoid Xeno-Free Growth Medium
(RC03-GM)
- 1 x 100 mL, RocketCell™ IO XF Growth Medium
- 1 x 50 µL, RocketCell™ Cell Viability Enhancer
Protocols and Resources
RocketCell™ Apical-Out Intestinal Organoid Xeno-Free Growth Kit – Protocol
Product Documentation
RocketCell™ Apical-Out Intestinal Organoid Xeno-Free Growth Kit – Sale Sheet
Product Data Sheet
Material Safety Data Sheet (MSDS)
Data and References
Figure 1. Use of VitroGel® hydrogels to generate iPSC-derived intestinal organoids.
Representative image showing iPSC-derived intestinal organoids cultured in VitroGel® ORGANOID. iPSC cells were cultured in VitroGel® STEM to generate spheroid cells. The spheroids were then differentiated into intestinal organoids using CytoGrow™ growth factors. (A)Yellow fluorescence: Phalloidin, (B) Magenta: Villin protein, (C) Blue staining (DAPI) represents cell nuclei. (D) Merged image of the iPSC-derived intestinal organoid
| Product Format | Complete Kit, Growth Medium |
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