Yes, VitroGel can be injected before or after soft hydrogel formation for in vivo study.
Before the hydrogel formation: The VitroGel solution can be injected directly into the animal. It will become a hydrogel when it encounters the ionic compounds of the physiological environment.
After soft hydrogel formation: The VitroGel hydrogel has an advanced injectable property after soft hydrogel formation. Mixing the hydrogel solution and cell culture media/PBS at a proper ratio, the final hydrogel becomes injectable for in vivo studies. Using this method, cells or other chemical compounds can be mixed in the hydrogel before injection.