Room Temperature Operation
Room temperature protocol/operation. No ice bucket required.
VitroGel® RGD High Concentration
RGD modified – tunable, xeno-free hydrogel – high concentration (3 mL kit)
VitroGel® RGD High Concentration
Tunable, xeno-free hydrogel with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions
during the 3D cell culture.
Xeno-free
100% synthetic. Animal & human origin-free, biofunctional hydrogel.
Tunable Hydrogel Strength
Adjust the hydrogel strength from 10 Pa to over 20,000 Pa to create the optimal cell environment.
Mix & Match
Build and create a customized multifunctional hydrogel by blending different types of VitroGel® together.
Easy-to-use No cross-linking agent required. Adjust hydrogel with Dilution Solution, mix with cells, add medium and incubate.
Easy cell harvesting Simple and efficient cell harvesting by the non-enzymatic VitroGel® Organoid Recovery Solution.
VitroGel® RGD High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. VitroGel® RGD High Concentration comes with VitroGel® Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa.
VitroGel® High Concentration hydrogels are our xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with our VitroGel® Organoid Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.
From 3D cell culture to 2D cell coating and animal injection, VitroGel® enables the seamless integration of in vitro and in vivo studies using the same platform system.
Mix & Match – 3D Cell Culture Your WAY!
Unique to VitroGel® High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel® RGD High Concentration can be “mix & matched” with other VitroGel High Concentration hydrogels such as VitroGel® IKVAV, VitroGel® YIGSR, VitroGel® MMP, and VitroGel® COL to create a customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists can customize their 3D culture microenvironment for various applications.
Specifications
| Contents | VitroGel® RGD High Concentration, 3 mL VitroGel® Dilution Solution, 50 mL |
| Hydrogel Formulation | Xeno-free tunable hydrogel modified with RGD peptide. |
| Use | Good for adhesion cells or cells requiring stronger cell-matrix interactions. |
| Mix & Match | Can be blended with other versions of VitroGel® concentrated hydrogels to create a custom multi-functional matrix. |
| Operation | Room temperature |
| Hydrogel Strength | 10 to 4,000 Pa of G’ depending on dilution ratio. Dilute with VitroGel® Dilution Solution (TYPE 1 or TYPE 2) for different concentrations. |
| pH | Neutral |
| Color | Transparent |
| Cell Harvesting | VitroGel® Organoid Recovery Solution 5-15 min cell recovery |
| Injectable | Injectable hydrogel |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Number of Uses | Dilution ratio: 1:2 = 225 uses at 50 µL per well 1:3 = 300 uses at 50 µL per well 1:5 = 450 uses at 50 µL per well |
3D Cell Culture Process in 20 Minutes
VitroGel® High Concentration hydrogels are easy to use. There is no cross-linking agent required. Work confidently at room temperature.
Tunable Hydrogel Strength
Simply diluting the hydrogel controls the gel strength.
Video Protocols & Demonstrations
Webinars
Research Highlights
Research Highlights
Game Changer for Lymphoma Therapy Screening
Research Highlights
An Artificial Womb for the Womb
Application Notes
Application Notes
3D Invasion of Glioblastoma Cells in VitroGel® Hydrogel System
Application Notes
Long-Term Neuron Culture Maturation in 3D Hydrogel Constructs
Application Notes
3D Cell Culture of Human Colon Cancer Cells (HCT116) on VitroGel® System
Application Notes
Long Term 3D Tumor Spheroid Culture in VitroGel® Hydrogel Matrix
Data and References
Cell Type Behavior Reference Table – VitroGel® RGD
Multiple studies have made use of RGD hydrogel in different tissues and cell types. RGD is commonly used as an immobilized, adhesive ligand in 3D hydrogels, allowing researchers to study many different cellular processes and behaviors in normal physiological and pathological contexts.
Bone
| Cell Type | Behavior |
|---|---|
| Goat bone marrow stromal cells | Promoted osteogenic differentiation |
| Rat bone marrow stromal cells | Promoted osteogenic differentiation |
| Rat osteoblasts | Increased cell attachment and spreading |
Cancer/Tumor
| Cell Type | Behavior |
|---|---|
| Biphasic synovial sarcoma SYO-1 | Cell proliferation and cell matrix interactions |
| Bone OSA 1777 | Cell proliferation and cell matrix interactions |
| Breast 4T1 | Cell proliferation, division, migration, and invasion |
| Breast AU-565 | Cell proliferation and cell matrix interactions |
| Breast Cancer MCF-7 | Cell proliferation, intercellular connections |
| Breast E0771 | Cell proliferation and cell matrix interactions |
| Breast MDA-MB-231 | Cell proliferation, division, migration, and invasion |
| Breast T47D | Cell proliferation, division, migration, and invasion |
| Colorectal adenocarcinoma DLD-1 cells | Cell proliferation and cell matrix interactions |
| Epithelial ovarian OV-MZ-6 | Promoted spheroid formation and proliferation |
| Epithelial ovarian SKOV-3 | Promoted spheroid formation and proliferation |
| Fuji Cells | Cell proliferation and cell matrix interactions |
| Glioblastoma SF 268 | Cell proliferation and cell matirx interaction |
| Glioblastoma SF 295 | Cell proliferation and cell matirx interaction |
| Glioblastoma SNB75 | Cell proliferation and cell matirx interaction |
| Glioblastoma U-251 MG | Cell proliferation and cell matirx interaction |
| Glioma U87-MG | Increased cell spreading and actin stress fiber assembly |
| Glioma U87-MG | Cell proliferation and cell matirx interaction |
| Glioma U373-MG | Increased cell adhesion duration and migration (on higher stiffness) |
| HEK 293 | Cell proliferation and cell matrix interactions |
| Huaman colon carcinoma HCT-8 | Cell proliferation and cell matirx interaction |
| Human colorectal carcinoma HCT 116 | cell proliferation, cell survival, and intercelluar networking |
| Human pancreatic cancer PANC-1 | cell proliferation and cellular interactions |
| Insulinoma ins-1 (Rat) | Cell proliferation and cell matrix interactions |
| Liver carcinoma HepG2 | Cell proliferation and cell matirx interaction |
| Melanoma Cells | Cell proliferation and cell matrix interactions |
| Ovarian carcinoma OVCAR-3 | Cell proliferation and invasion |
| Primary glioblastom U87 | cell proliferation and cellular interactions |
| Prostate adenocarcinoma LNCaP | Increased cell attachment |
| Prostate CRPC | Cell proliferatin and invasion |
| Prostate DU145 | Cell proliferation and invasion |
| Prostate PC3 | Cell proliferation and invasion |
Cartilage
| Cell Type | Behavior |
|---|---|
| Bovine chondrocytes | Increased cell viability and proliferation |
| Bovine chondrocytes | Promoted cell attachment, viability, and stress fiber formation |
| Human chondrocytes | Promoted cell viability and proliferation |
Connect Tissues
| Cell Type | Behavior |
|---|---|
| Fibroblast NIH3T3 | Promoted cell spreading |
| Fibroblasts NIH3T3 | Increased directional cell migration toward gradient |
| Human dermal fibroblasts | Promoted cell survival and spreading |
| Human dermal fibroblasts | Increased cell adhesion and proliferation |
| Human foreskin fibroblasts | Promoted cell spreading |
Epithelial Cells
| Cell Type | Behavior |
|---|---|
| A549 cells | Cell proliferation and invasion |
| MCF-12A | Cell proliferation and invasion |
| Mouse ovarian follicle cells | Cell proliferation and invasion |
Immune Cells
| Cell Type | Behavior |
|---|---|
| Beta TC3 Cells | Cell proliferation and cellular interactions |
Kidney
| Cell Type | Behavior |
|---|---|
| Human embryonic kidney HEK293 | Promoted spheroid formation |
| Madin-Darby Canine Kidney | Promoted formation of structured epithelial cysts |
Liver
| Cell Type | Behavior |
|---|---|
| Human hepatocytes | Increased number of filopodia and synthesis of albumin |
| Mouse hepatocytes | Promoted cell viability |
Lung
| Cell Type | Behavior |
|---|---|
| Alveolar epithelial A549 | Inhibited cell detachment |
| Alveolar epithelial RLE-6TN | Increased cell attachment and mesenchymal differentiation |
Muscle
| Cell Type | Behavior |
|---|---|
| Mouse skeletal myoblasts | Promoted cell attachment, proliferation, and myofibril formation |
| Myoblasts C2C12 | Promoted cell proliferation and differentiation |
Neural
| Cell Type | Behavior |
|---|---|
| Chick dorsal root ganglion cells | Increased neurite length, neurite outgrowth, and neurite number |
| In vivo lesioned rat cortex | Supported angiogenesis and inhibited glial scars |
| In vivo lesioned rat spinal cord | Supported angiogenesis and axon regeneration |
Stem Cells
| Cell Type | Behavior |
|---|---|
| Human embryonic stem cells | Increased retinal pigmented epithelium and optic vesicle development |
| Human iPSC | Cell proliferation, and cell matrix interactions |
| Human mesenchymal stem cells | Increased cell viability |
| Mouse embryonic stem cells | Promoted endothelial cell differentiation |
| Mouse mesenchymal stem cells | Promoted cell spreading and migration |
| Rat mesenchymal stem cells | Increased cell adhesion and spreading |
| Rat mesenchymal stem cells | Promoted cell attachment and differentiation |
Vascular/Cardiac
| Cell Type | Behavior |
|---|---|
| Human aortic smooth muscle cells | Promoted cell attachment |
| Human umbilical vein endothelial cells | Increased cell adhesion, proliferation, migration, and angiogenesis |
| Human umbilical vein endothelial cells | Increased cell adhesion and proliferation |
| Rat neonatal cardiac | Promoted cell attachment and tissue regeneration and prevented apoptosis |
View All Cell Type Behaviors for All VitroGel Products
| TISSUE/ORGAN TYPE | CELL TYPE | READY TO USE | HIGH CONCENTRATION | BEHAVIOR | |
|---|---|---|---|---|---|
| Beta Cell | BL5 human beta cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D VitroGel® MMP | Enhance spheroids formation | |
| Beta TC3 cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cellular interactions | ||
| Bone | Bone marrow stromal cells (rat) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Osteogensic differentiation Cell attachment and osteoblast differentiation Cell proliferation cell viability and cellular networking | |
| Osteoblasts (rat) | VitroGel® MSC VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell attachment and spreading | ||
| Bone marrow stromal cells (bovine) | VitroGel® MSC VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell spreading and osteocalcin expression | ||
| Breast | Mammary gland MCF10A | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitrolGel RGD VitroGel® COL VitroGel® MMP | Spheroid formation MMP activity in response to TGF-B1 | |
| Mammary epithelium (mouse) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell invasion and dissemination | ||
| Cancer/Tumor | Human colorectal carcinoma HCT 116 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation cell survival and intercellular networking | |
| Huaman colon carcinoma HCT-8 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Glioma U87-MG | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP VitroGel® COL | Cell spreading and acting stress fiber assembly cell proliferation spreading and migration Cell migration dependent on mechancial force Cell proliferation and cell matrix interaction | ||
| Gliobastoma SF 268 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitrolGel RGD | Cell proliferation and cell matrix interaction | ||
| Gliobastoma SF 295 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Glioblastoma SNB75 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Glioblastoma U-251 MG | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Prostate PC3 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® COL VitroGel® IKVAV VitroGel® RGD VitroGel® MMP | Cell proliferation reduced MMP release invasion migration and spheroid metabolic activity. | ||
| Prostate LNCaP | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell attachment proliferation and prostate specific antigen release | ||
| Prostate CRPC | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and invasion | ||
| Prostate DU145 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and invasion | ||
| Melanoma B16F10 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® COL VitroGel® YIGSR | Cell migration invasion MMP release cell attachment and spreading | ||
| Breast MDA-MB-231 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP VitroGel® 3D | Cell invasion spreading proliferation division migration and cluster growth | ||
| Fibrosarcoma HT1080 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell infiltration attachment | ||
| Breast T47D | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® COL VitroGel® 3D VitroGel® RGD VitroGel® MMP | Force dependent tubule formation cell cluster growth spheroid formation and proliferation | ||
| Breast 4T1 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation | ||
| Breast CTC | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D VitroGel® RGD | Cell proliferation | ||
| Breast E0771 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation spheroid formation | ||
| Brest AU-565 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation cell matrix interactions | ||
| Epithelial ovarian OV-MZ-6 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Spheroid formation and proliferation | ||
| Epithelial ovarian SKOV-3 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Spheroid formation and proliferation | ||
| Glioma U373-MG | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell adhesion invasion and migration | ||
| Rhabdomyosarcoma (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® YIGSR | Cell attachment and spreading | ||
| Melanoma SK-MEL-28 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Cell adhesion and proliferation | ||
| Melanoma K-1735 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Cell invasion | ||
| Melanoma A2058 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Collagenolytic activity | ||
| Brainstem glioma DIPG | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell proliferation and survival | ||
| Hela Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D VitroGel® RGD VitroGel® MMP | Cell proliferation | ||
| Colorectal adenocarcinoma DLD-1 cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Giloma LRM55 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® IKVAV VitroGel® MMP | Cell attachment | ||
| Melanoma WM 239A | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell invasion | ||
| Melanoma Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Insulinoma ins-1 (Rat) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Biphasic synovial sarcoma SYO-1 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation cell matrix interation and cell survival | ||
| Fuji Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and cell matrix interaction | ||
| Chordoma Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Cell proliferation | ||
| Bone OSA 1777 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Spheroid and cluster formation | ||
| Glioma RuGli | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® COL | Integrin dependent cell adhesion | ||
| Breast Cancer MCF-7 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP VitroGel® 3D | Cell proliferation intercellular connections morphological changes MMP expression and angiogenesis | ||
| Liver carcinoma HepG2 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell viability growth drug resistance proliferation and cellular matrix interaction | ||
| Human pancreatic cancer PANC-1 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell proliferation and cellular interactions | ||
| Primary breast (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell invasion migration and dissemination | ||
| Ovarian carcinoma OVCAR-3 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP | Cell proliferation cell matrix interactions | ||
| Ovarian OVCA429 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP VitroGel® COL | MMP dependent cell invasion | ||
| Human Osteosarcoma KHOS | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® 3D | Cell proliferation and spheroids formation | ||
| Human Osteosarcoma U2OS | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® 3D | Cell proliferation and spheroids formation | ||
| Human fibroblast-like synoviocytes (FLS) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Cell proliferation and inflammatory responses | ||
| Human Liposarcoma 94T778 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Cell proliferation and spheroids formation | ||
| Human diffuse large B-cell lymphoma (DLBLC) SUDHL-10 | VitroGel® Hydrogel Matrix | Cell viability growth drug resistance proliferation and cellular matrix interaction | |||
| Priess human lymphoblastoid cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Enhance spheroids and cluster formation and promote cell viability. | ||
| Cartilage | Chondrocytes (bovine) | VitroGel® MSC VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell viability and proliferation | |
| Chondrocytes (human) | VitroGel® MSC VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell viability and proliferation | ||
| Connective Tissue | Dermal Fibroblasts (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell viability and spreading | |
| Fibroblasts NIH3T3 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Directional cell migration toward gradient and cell spreading dependent on substrata rigidity | ||
| Foreskin fibroblasts (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® YIGSR VitroGel® MMP | Cell spreading substrata degradation and cell invasion | ||
| Skin fibroblasts (skin) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® IKVAV | Cell adhesion | ||
| Epidermal keratinocytes | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell viability | ||
| Epithelial Cells | Mouse ovarian follicle cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | 3D cell culture using ES-hydrogel can enhance vitro follicle culture by considering the permeability and stiffness of the gel. | |
| Human Nthy-ori 3-1 cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Enhance spheroids and cluster formation and promote cell viability. | ||
| A549 cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Enhance cell proliferation and cell matrix interactions. | ||
| MCF-12A | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Enhance cell proliferation and cell matrix interactions. | ||
| Immortalized bronchial epithelial cells HBEC-KRAS | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® 3D | Cell proliferation | ||
| Eye | Corneal endothelial B4G12 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit VitroGel® Angiogenesis Assay | VitroGel® RGD VitroGel® Angiogenesis Assay HC kit | Cell attachment and spreading | |
| Retinal ganglion cells (xenopus) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Neurite outgrowth | ||
| Immune Cells | CD8 + T cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Enhance spheroids and cluster formation and promote cell viability. | |
| Kidney | Human embryonic kidney HEK293 | VitroGel® Hydrogel Matrix VitroGel® HEK293 VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | 3D spheroids formation | |
| Madin-Darby Canine Kidney | VitroGel® Hydrogel Matrix VitroGel® HEK293 VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® MMP | Epithelial cysts formation | ||
| Podocytes (human) | VitroGel® Hydrogel Matrix VitroGel® HEK293 VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Glomerular capillary formation | ||
| glomerular endothelial cells (human) | VitroGel® Hydrogel Matrix VitroGel® HEK293 VitroGel® ORGANOID Disovery Kit VitroGel® Angiogenesis Assay | VitroGel® RGD VitroGel® Angiogenesis Assay HC kit | Glomerular capillary formation | ||
| Liver | Hepatocytes (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Filopodia formation and synthesis of albumin and cell attachment | |
| Hepatocytes (mouse rat swine) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® MMP | Cell viability spearding Albumin secretion | ||
| Lung | Alveolar basal epithelial A549 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell attachment | |
| Alveolar epithelial RLE-6TN | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell attachment and mesenchymal differentiation | ||
| Pulmonary fibroblasts LL2 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® IKVAV | Cell adhesion | ||
| HFL1 lung fibroblasts CCL153 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell proliferation and spindle morphology | ||
| Lung cancer associated fibroblasts (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Substrata contractility | ||
| Lung fibroblasts MCR-5 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | NGF-mediated substrata contraction | ||
| Muscle | Myoblasts C2C12 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell proliferation differentiation attachment myofibril formation myotube formation and integrin dependent cell adhesion | |
| Skeletal myoblasts (mouse) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD | Cell attachment proliferation and myofibril formation | ||
| Myoblasts (human) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell adhesion alignment along fiber and myotube formation | ||
| Myoblasts C25Cl48 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL | Cell proliferation differentiation and myotube formation | ||
| Neural | Dorsal root ganglion (chick) | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit VitroGel® NEURON | VitroGel® RGD VitroGel® COL | Neurite formation and force dependent neurite outgrowth | |
| Neural PC12 | VitroGel® Hydrogel Matrix | VitroGel® RGD VitroGel® IKVAV | Neurite outgrowth | ||
| Neural stem cell/ progenitor cell (rat) | VitroGel® STEM VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV | Cell viability attachment and differentiation | ||
| Neural stem cell/ progenitor cell (human) | VitroGel® STEM VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV VitroGel® COL | Cell viability attachment and differentiation | ||
| Schwann cells (rat) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD | Cell attachment and migration | ||
| Neural stem cell/ progenitor cell (mouse) | VitroGel® STEM VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV | Cell adhesion and differentiation | ||
| Cortical astrocytes (rat) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV | Cell adhesion | ||
| Spiral ganglion neurons (mouse) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® IKVAV | Neurite outgrowth | ||
| Motor neurons (human) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Force dependent neurite outgrowth | ||
| Forebrain neurons (human) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Force dependent neurite outgrowth | ||
| Cortical neurons (rat) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Neuronal viability and neurite outgrowth | ||
| Dorsal root ganglion (rat) | VitroGel® Hydrogel Matrix VitroGel® NEURON | VitroGel® RGD VitroGel® COL VitroGel® IKVAV | Neurite outgrowth | ||
| Red Blood Cells | Red Blood Cells | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® 3D | Enhance Spheroids and cluster formation and promote cell viability | |
| Pancreas | B-cells MIN6 | VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® IKVAV | Reduced apoptosis and increased insulin release | |
| Stem Cells | Mesenchymal stem cells (human) | VitroGel® MSC | VitroGel® RGD VitroGel® COL VitroGel® IKVAV VitroGel® MMP | Cell viability proliferation differentiation neuronal differntiation neurite outgrowth attachment spreading viability and osteoblast differentiation | |
| Mesenchymal stem cells (mouse) | VitroGel® MSC | VitroGel® RGD VitroGel® MMP | Cell spreading and migration | ||
| Mesenchymal stem cells (rat) | VitroGel® MSC | VitroGel® RGD | Cell adhesion and spreading | ||
| Embryonic stem cells (mouse) | VitroGel® STEM VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® COL VitroGel® YIGSR | Endothelial cell differentiation neuronal differentiation and neurite outgrowth | ||
| Induced pluripotent stem cells (human) | VitroGel® STEM VitroGel® ORGANOID Disovery Kit | VitroGel® RGD VitroGel® YIGSR VitroGel® IKVAV | Cell viability | ||
| Human stem cells from apical papilla SCAP | VitroGel® STEM | VitroGel® RGD | Cell viability | ||
| Hematopoietic Stem Cells | VitroGel® STEM | Cell viability | |||
| Adipose derived stem cells (human) | VitroGel® MSC | VitroGel® RGD VitroGel® 3D VitroGel® IKVAV | Cell viability cell attachment | ||
| Vascular/cardiac | Umbilical vein endothelial cells | VitroGel® Angiogenesis Assay VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® Angiogenesis Assay HC kit | Cell attachment proliferation migration angiogenesis gene expression changes migratory cell infiltration cell survival and VEGF dependent migration | |
| Neonatal cardiac (rat) | VitroGel® Angiogenesis Assay VitroGel® Hydrogel Matrix VitroGel® ORGANOID Disovery Kit | VitroGel® Angiogenesis Assay HC kit | Cell attachment tissue regeneration and attachment similar to laminin | ||
| Aortic smooth muscle cells | VitroGel® Hydrogel Matrix | VitroGel® Angiogenesis Assay HC kit | Cell attachment | ||
| Endothelial (human) | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Cell differentiation | ||
| Endotheliocytes | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Cell migration | ||
| Microvascular endothelial cells (human) | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Cell mobility | ||
| Aortic endothelial cells (bovine) | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Force dependent cell spreading | ||
| Capillary endothelial cells (bovine) | VitroGel® Angiogenesis Assay | VitroGel® Angiogenesis Assay HC kit | Capillary like network formation |
Data
Figure 1. Rheological properties of VitroGel® RGD with DMEM medium.
A) – C) The gel formation curve after mixing with DMEM (A), DMEM/F-12 (B), and RPMI (C) media. VitroGel RGD was diluted at 1:0,1:1, 1:2 and 1:3 (v/v) with VitroGel Dilution Solution (Type 1) and then mix with media at 4:1 (v/v) ratio; D) – F) The gel strength after 24 hrs incubation in DMEM (D), DMEM/F-12 (E), and RPMI (F) media. The hydrogel was prepared as method A and incubated at 37°C CO2 incubator for 24 hrs before the rheological test. (10 ~ 4000 Pa of G’ of regular products at dilutions. Customized high concentration product to reach over 20K Pa)
Figure 2. 3D culture of OP9 cells in VitroGel® RGD.
Hydrogel was prepared at 1:3 dilution with VitroGel® Dilution Solution (Type 1). The images were taken on days 2 and 7. VitroGel® RGD shows support for OP9 cell proliferation and cell-cell communication. The stronger cell-matrix interactions help the cells to form the cell-networking structure.
Figure 3. 3D view of OP9 cells growth in VitroGel® RGD.
Cell networking structure formed in VitroGel® RGD.
Figure 4. 3D culture of U87-MG cells in VitroGel® RGD.
Cells can grow in 3D hydrogel at 1:1 and 1:3 dilution of VitroGel® RGD. U87-MG cells exhibit a cell networking structure and morphology in VitroGel® RGD, indicating cell-cell and cell-matrix interactions.
References/Publications
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| High Concentration Kit Type | VitroGel RGD + Dilution Solution TYPE 1, VitroGel RGD + Dilution Solution TYPE 2 |
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