VitroGel® RGD-PLUS

    xeno-free hydrogel – high concentration of RGD modified
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    • 2 mL
    • 10 mL
    Clear
    SKU: TWG003 Category:

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    Overview

    VitroGe®l RGD-PLUS is a ready-to-use tunable hydrogel system modified with a high concentration of RGD cell adhesive peptide, promoting the cell attachment and cell-matrix interactions during the 3D cell culture. VitroGel RGD-PLUS has 3X more RGD peptide compared to VitroGel 3D-RGD, resulting in a higher level of integrin binding activities to promote intercellular network even after hydrogel dilution.

    VitroGel RGD-PLUS is one system of the family of ready-to-use, xeno-free tunable hydrogel system which closely mimics the natural extracellular matrix (ECM) environment.

    VitroGel creates a functional and optimized environment to make cells feel like at home. The hydrogel system is room temperature stable, has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a tunable hydrogel matrix by simply mixing with cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

    From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.

     

    Specifications

    • Xeno-free tunable hydrogel modified with a high concentration of RGD peptide
    • Support strong cell-cell communication; Good for adhesion cells or cells requiring stronger cell-matrix interactions
    • Ready-to-use at room temperature
    • Tunable hydrogel:  Dilute with VitroGel Dilution Solution (TYPE 1 or TYPE 2) for different concentrations
    • Neutral pH
    • Transparent
    • Compatible with VitroGel Cell Recovery Solution for easy cell harvesting
    • Injectable hydrogel (Check user handbook for preparation details)
    • Ships room temperature. Store at 2-8°C
    • Size: 2 mL and 10 mL
    • Number of uses (10 mL): 2-6 of 24-well plate at 250 to 300 µL/well

    Handbook and Resources

    Product Documentation

     

    Video Resources

    Application Notes

    Data and References

    Figure 1.

    3D culture of OP9 cells in both VitroGel 3D-RGD and VitroGel RGD-PLUS. (both hydrogels have been prepared at 1:3 dilution with VitroGel Dilution Solution (Type 1), The images were taken on day 2 and 7) VitroGel RGD-PLUS has better support for OP9 cell proliferation and cell-cell communication. The stronger cell-matrix interactions help the cells to form the cell-networking structure.

    Figure 2.

    3D view of OP9 cells growth in VitroGel 3D-RGD and VitroGel RGD-PLUS. Cell networking structure formed in VitroGel RGD-PLUS and only single cell suspension has been observed in VitroGel 3D-RGD.

    Figure 3.

    3D culture of U87-MG cells in both VitroGel 3D-RGD and VitroGel RGD-PLUS. Cells can grow in 3D hydrogel at 1:1 and 1:3 dilution of both hydrogel. However, the morphologies of the 3D cell culture are very different. Unlike the spheroids structure in VitroGel 3D-RGD, U87-MG cells perform better cell networking structure and the cell morphology in VitroGel RGD-PLUS indicates a better cell-cell and cell-matrix interaction.

    Tables of successful cell types

    Cell typesApplicationsCulture mediumDilution
    4T1 cells3D cultureRPMI 1640 with 10% FBS1:2
    A549 cells3D cultureDMEM with 10% FBS1:1 to 1:3
    Au565 cells3D cultureRPMI 1640 with 10% FBS1:3
    Beta TC3 cells3D cultureDMEM with 10% FBS1:3
    BT 474 cells3D cultureDMEM with 10% FBS1:3
    DLD1 cells3D cultureDMEM with 10% FBS1:1 to 1:3
    Fuji cells3D cultureRPMI 1640 with 10% FBS1:1 to 1:3
    H460 cells3D cultureRPMI 1640 with 10% FBS1:1 to 1:3
    HCT 116 cells2D and 3D cultureMcCoys’ 5 with 10% FBS1:1
    HCT-8 cells3D cultureRPMI 1640 with 10% FBS1:3
    HEK 293 cells3D cultureDMEM with 10% FBS1:3
    Hela cells2D and 3D cultureDMEM with 10% FBS1:3
    HepG2 cells3D cultureDMEM with 10% FBS1:1 to 1:3
    Human iPSCs2D and 3D culturemTeSR11:3
    Ins-1 Cells3D cultureRPMI 1640 with 10% FBS1:3
    MCF-7 cells2D and 3D cultureDMEM with 10% FBS1:1 to 1:3
    MCF-12A cells3D cultureDMEM/F-12 with 10% FBS1:3
    MDA-MB-231 cells3D cultureL-15 medium with 10% FBS1:1 to 1:3
    Melanoma cells3D cultureRPMI 1640 with 10% FBS1:1 to 1:3
    OP9 cells2D and 3D cultureAlpha-MEM with 10% FBS1:3
    OVCAR-3 cells3D cultureRPMI 1640 with 10% FBS1:1 to 1:3
    Panc-1 cells3D cultureDMEM with 10% FBS1:1 to 1:3
    SF 268 cells2D and 3D cultureRPMI 1640 with 10% FBS1:1 to 1:3
    SF 295 cells2D and 3D cultureRPMI 1640 with 10% FBS1:1 to 1:3
    SKB3 cells2D and 3D cultureDMEM with 10% FBS1:1 to 1:3
    SNB 75 cells2D and 3D cultureRPMI 1640 with 10% FBS1:1 to 1:3
    SYO-1 cells3D cultureRPMI 1640 with 10% FBS1:1 to 1:3
    T47D cells3D cultureRPMI 1640 with 10% FBS1:3
    U87-MG cells2D and 3D cultureEMEM with 10% FBS1:1 to 1:3
    UD 145 cells3D cultureEMEM with 10% FBS1:1 to 1:3
    U-251 MG cells2D and 3D cultureEMEM with 10% FBS1:1 to 1:3

    References

    Size

    2 mL, 10 mL