Conferences, Poster Presentations
Generation of Apical-out Intestinal Organoids Using Xeno-free VitroGel® ORGANOID
Poster No. P02-06-26 at NUTRITION 2026
- Poster Board Number: C-46
- Poster Session: Applications of Novel in vitro Models in Nutrition Research
- Presentation Date: July 25, 2026
- Presentation Time: 11:30 A.M. -12:30 P.M.
- Presenter: Kalhara Menikdiwela, Ph.D
Background:
- Organoids are 3D self-organizing tissue models that closely mimic the architecture and function of native organs.
- Epithelial polarity is a fundamental feature of intestinal organoids, governing nutrient absorption, drug transport, and barrier function.
- Conventional Matrigel-grown intestinal organoids exhibit an apical-in orientation, restricting direct access to the absorptive epithelial surface.
- Apical-out intestinal organoids expose the brush border to the external environment, enabling direct interaction with nutrients, drugs, microbes, and
other luminal factors. - Apical-out polarity improves the physiological relevance of in vitro models for studying intestinal biology, nutrient uptake, and oral drug absorption.
- Most current organoid culture systems rely on animal-derived extracellular matrices, which introduce batch-to-batch variability and limit translational
applications. - Synthetic, xeno-free hydrogels provide a chemically defined and reproducible microenvironment for organoid culture, improving experimental consistency
and scalability. - Developing robust apical-out organoid models in synthetic hydrogels can enhance preclinical research in nutrition, drug discovery, disease modeling, and host–microbiome interactions.
Please complete the form below to request the poster. Available on July 25, 2026.
Products Used:
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