Animal Origin-Free N2-like Supplement
NEW
RocketCell™ S2 Supplement (100X), AOF
Animal Origin-Free N2-like Supplement
RocketCell™ S2 Supplement (100X), AOF
Precision Minimalist Formula
A tightly defined, low-complexity formulation provides a clean, low-noise environment optimized for neuronal selectivity and signaling studies.
Neural Stem / Progenitor Cell Support
Validated component in neural induction media (NIM) protocols for transitioning hES/iPSC cells toward neuroepithelial precursors.
Neuronal Selection Utility
Selectively supports postmitotic neurons in mixed primary cultures while suppressing overgrowth of non-neuronal cell populations.
Animal Origin-Free Recombinant Components
Formulated exclusively with recombinant and high-purity synthetic ingredients for consistent, lot-to-lot reproducibility.
Broad Application Range
Supports serum-free proliferation across a wide range of cell types and model systems, including glial models, pituitary lines, and select cancer lines, as well as organoid and tumoroid applications.
RocketCell™ S2 Supplement, AOF is an animal origin-free N2-like supplement providing a minimalistic, precisely controlled serum-free environment for neuronal and non-neuronal cell culture. Formulated exclusively with recombinant and high-purity synthetic components, it delivers the consistency and traceability that modern neural and stem cell research demands.
Ideal for serum-free adaptation, neural induction, glial selection, and a wide range of non-neuronal cell line applications.
Protocols and Resources
Protocol
Product Documentation
Sale Sheet
Product Data Sheet
Material Safety Data Sheet (MSDS)
Data and References
Organoids
Mouse Intestinal Organoid Cultured using RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF
Confocal fluorescence imaging of mouse intestinal organoids.
Mouse intestinal organoids were cultured using RocketCell™ S1 & S2 Supplements, AOF and stained to assess morphology and structural integrity. (A) Nuclear localization visualised by fluorescent staining (yellow). (B) F-actin/membrane visualization highlighting crypt-like budding and luminal architecture (cyan). (C) Merged overlay of nuclear (yellow) and structural (cyan) channels demonstrating organized cellular distribution. (D) Maximum intensity projection / representative z-frame highlighting nuclear density across the 3D organoid volume. Scale bars = 50 µm.
Cryo-Recovery and Re-Culturing Intestinal Organoid with RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF
Cryo-recovery and re-culturing of intestinal organoids using RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF.
Representative bright-field images show cryopreserved intestinal organoids following recovery at Day 0 (A) and subsequent growth at Day 12 (B) in VitroGel®. Quantitative analysis demonstrates progressive organoid expansion over 12 days across three replicate wells, indicating successful recovery and sustained growth following cryopreservation.
| Size | 5 mL |
|---|
Related products
NEW
Culture Media and Supplements
Animal Origin-Free B27-like Supplement
NEW
Culture Media and Supplements
Maximize cell recovery and growth during passaging, thawing, and cryopreservation.
NEW
Culture Media and Supplements
Animal Origin-Free N2B27 Like Premixed Supplement
New
Cell Culture Media (Animal Origin-Free)
Animal origin-free medium for 2D and 3D expansion of pluripotent stem cells.
New
Cell Culture Media (Animal Origin-Free)
One Core Medium. Endless Organoid Possibilities. An animal origin-free medium that supports multiple organoid types - just add growth factors.
New
Cell Culture Media (Animal Origin-Free)
Animal origin-free complete medium for 3D and 2D neural stem cell culture
