N2-like Animal Origin-Free Supplement
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RocketCell™ S2 Supplement (100X), AOF
N2-like Animal Origin-Free Supplement
RocketCell™ S2 Supplement (100X), AOF
Precision Minimalist Formula
A tightly defined, low-complexity formulation provides a clean, low-noise environment optimized for neuronal selectivity and signaling studies.
Neural Stem / Progenitor Cell Support
Validated component in neural induction media (NIM) protocols for transitioning hES/iPSC cells toward neuroepithelial precursors.
Neuronal Selection Utility
Selectively supports postmitotic neurons in mixed primary cultures while suppressing overgrowth of non-neuronal cell populations.
Animal Origin-Free Recombinant Components
Formulated exclusively with recombinant and high-purity synthetic ingredients for consistent, lot-to-lot reproducibility.
Broad Application Range
Supports serum-free proliferation across a wide range of cell types and model systems, including glial models, pituitary lines, and select cancer lines, as well as organoid and tumoroid applications.
RocketCell™ S2 Supplement, AOF is an animal origin-free N2-like supplement providing a minimalistic, precisely controlled serum-free environment for neuronal and non-neuronal cell culture. Formulated exclusively with recombinant and high-purity synthetic components, it delivers the consistency and traceability that modern neural and stem cell research demands.
Ideal for serum-free adaptation, neural induction, glial selection, and a wide range of non-neuronal cell line applications.
Challenges We Solve
Common Challenges
Undefined or animal-derived components in conventional supplements.
Variability in neural stem and progenitor cell expansion.
Inconsistent neural differentiation outcomes.
Limited serum-free culture options across diverse cell and model systems.
How RocketCell™ S2 Supplement, AOF Solves The Issues
Animal origin-free formulation
Provides a fully defined, animal origin-free N2-Like formulation for more consistent cell culture workflow.
Reproducible neural cell culture
Supports robust expansion and maintenance of neural stem and progenitor cells.
Consistent differentiation workflow
Provides defined supplementation to support reproducible neural differentiation workflow.
Broad serum-free and animal origin-free culture compatibility
Supports proliferation across diverse cell types and model systems, including glial and pituitary models, select cancer cell lines, organoids, and tumoroids.
Protocols and Resources
Protocol
Data and References
Organoids
Mouse Intestinal Organoid Cultured using RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF
Confocal fluorescence imaging of mouse intestinal organoids.
Mouse intestinal organoids were cultured using RocketCell™ S1 & S2 Supplements, AOF and stained to assess morphology and structural integrity. (A) Nuclear localization visualised by fluorescent staining (yellow). (B) F-actin/membrane visualization highlighting crypt-like budding and luminal architecture (cyan). (C) Merged overlay of nuclear (yellow) and structural (cyan) channels demonstrating organized cellular distribution. (D) Maximum intensity projection / representative z-frame highlighting nuclear density across the 3D organoid volume. Scale bars = 50 µm.
Cryo-Recovery and Re-Culturing Intestinal Organoid with RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF
Cryo-recovery and re-culturing of intestinal organoids using RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF.
Representative bright-field images show cryopreserved intestinal organoids following recovery at Day 0 (A) and subsequent growth at Day 12 (B) in VitroGel®. Quantitative analysis demonstrates progressive organoid expansion over 12 days across three replicate wells, indicating successful recovery and sustained growth following cryopreservation.
| Size | 5 mL |
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