NEW
Clear glass vial with white cap, labeled 'RocketCell S1 Supplement (50x), AOF' for research use only, sterile
Fluorescent micrograph of a neural network: green cell bodies with magenta-colored neurites extending between them.
RocketCell™ S1 Supplement (50X), AOF - Image 3
Clear glass vial with white cap, labeled 'RocketCell S1 Supplement (50x), AOF' for research use only, sterile
Fluorescent micrograph of a neural network: green cell bodies with magenta-colored neurites extending between them.
RocketCell™ S1 Supplement (50X), AOF - Image 3

RocketCell™ S1 Supplement (50X), AOF

B27-like, Animal Origin-Free Supplement

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RocketCell™ S1 Supplement (50X), AOF

B27-like Animal Origin-Fre Supplement

Blue shield icon with a circular emblem in the center, representing security/protection.

Comprehensive Antioxidant protection
An optimized blend of antioxidant enzymes and vitamins that helps protect sensitive cells from oxidative damage, supporting high viability even at low plating densities.

3D-Validated Specifically optimized for use within VitroGel® hydrogel systems for spheroid and organoid applications. While optimized for VitroGel® hydrogels, it also supports animal-based ECMs cultures for spheroids, neuronal cultures, complex co-cultures, and organoid model development.

Drop-in replacement
Protocol-compatible with standard B27 workflows at equivalent working concentrations.

Retinoid-Competent Formulation
Supports baseline CNS neuronal maintenance, maturation, and long-term culture stability.

Blue line diagram showing a culture dish at the top with three small circular cells inside, and three arrows pointing to three distinct neural cell icons below (neuron-like shapes) indicating derivation or transfer from the dish.

Broad and Versatile
Supports organoid, tumoroid, hippocampal and cortical neuron cultures, iPSC-derived neuron maturation, cancer stem cell, and cardiomyocyte maintenance.

RocketCell™ S1 Supplement, AOF is an animal origin-free replacement for conventional B27 supplements, engineered for the long-term survival, maturation, and functional maintenance of CNS neurons and a broad spectrum of other cell types. All components are of recombinant, synthetic origin, eliminating the batch-to-batch variability and safety concerns inherent in animal-derived additives.

This is the all-purpose formulation — the right choice when the primary goal is maximizing long-term cell viability without directing cell fate. Supplied as a 50X liquid concentrate for use with standard neurobasal or serum-free basal media.

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Challenges We Solve

Common Challenges 

Lack of a reliable animal origin-free formulation to support GMP process.

Variability in culture conditions across experiments.

Unwanted signaling that can interfere with targeted differentiation.

Difficulty in maintaining long-term cultures

How RocketCell™ S1 Supplement, AOF Solves The Issues​

Animal origin-free formulation for GMP transition
B27- like AOF formulation that simply transition to scalable and controlled GMP workflows.

Standardized B27-like formulation
Provides a standardized formulation to support reproducible culture conditions across various tissue sources and cell models.

Abstract illustration of several rounded cells with dark centers clustered together, outlined in blue.

Formulations for controlled differentiation
Available in minus Insulin and minus Vitamin A formulations for greater control over differentiation protocols.

Sustained long-term cell survival
Supports cell viability during extended culture of advanced 3D models. Supports the maturation and maintenance of differentiated cell models, including iPSC-derived neurons and cardiomyocytes.

Data and References

ORGANOIDS

Cryo-Recovery and Re-Culturing Intestinal Organoid with RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF ​

Cryo-recovery and re-culturing of intestinal organoids using RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF.

Representative bright-field images show cryopreserved intestinal organoids following recovery at Day 0 (A) and subsequent growth at Day 12 (B) in VitroGel®. Quantitative analysis demonstrates progressive organoid expansion over 12 days across three replicate wells, indicating successful recovery and sustained growth following cryopreservation.​

Mouse Intestinal Organoid Cultured using RocketCell™ S1 (B27-like) & S2 (N2-like) Supplements, AOF ​

Panel A: Yellow fluorescent cell cluster with scattered bright dots on a black background; scale bar 50 μm.
Fluorescent image showing yellow-stained cell nuclei clustered in a dark field (panel D).

Confocal fluorescence imaging of mouse intestinal organoids.

Mouse intestinal organoids were cultured using RocketCell™ S1 & S2 Supplements, AOF and stained to assess morphology and structural integrity. (A) Nuclear localization visualised by fluorescent staining (yellow). (B) F-actin/membrane visualization highlighting crypt-like budding and luminal architecture (cyan). (C) Merged overlay of nuclear (yellow) and structural (cyan) channels demonstrating organized cellular distribution. (D) Maximum intensity projection / representative z-frame highlighting nuclear density across the 3D organoid volume. Scale bars = 50 µm.

SPheroids

iPSC-derived Neuroectodermal Spheroid Culture in VitroGel® Hydrogels
and the RocketCell™ S1 (B27-like) Supplement, AOF ​

Collage comparing ViTroGel STEM (top) and NEURON (bottom): left panels show round speckled gel disks, middle panels show grayscale electron micrographs of cells, right panels show close-up of a textured circular cell structure.
Blue nuclei stained with Hoechst in a dense cell cluster; scale bar shows 200 µm; dark background.

3D iPSC-derived Floor Plate Neuroepithelium Spheroids.

iPSC spheroids cultured with the RocketCell™ 3D iPSC Complete Growth Kit, AOF were transitioned into FPNE1 Medium, and cultured for 4 days, passaged into VitroGel® STEM or VitroGel® NEURON in FPNE medium supplemented with RocketCell™ S1 Supplement, Minus Insulin, AOF and characterized after 8 days. These spheroids expressed canonical FPNE markers such as FoxA2, Netrin, and Nkx2.2.​

Floor Plate Neuron Epithelium Spheroid Differentiation into Motor Neurons with the RocketCell™ S1 (B27-like) Supplement, AOF ​

A

Three-panel microscopy image: left shows a circular field densely populated with small dark particles; center and right show two large dark clusters with a fibrous halo.

B

Fluorescent micrograph of neuron networks in purple and green with a highlighted magnified inset on the left panel and a zoomed section on the right panel, showing dense neural connections.

Floor Plate Neuron Epithelium Culture Differentiation into Motor Neurons​

​A. FPNE cultures were transitioned to FPNE3 patterning medium for 4 days and transitioned to Neuron Differentiation Medium with the RocketCell™ S1 Supplement, AOF for 14 days. ​

​B. The cultures were fixed and processed for FL immunostaining using anti-phosphorylated neurofilament (purple) and CHAT (green) for motor neurons. This system demonstrates the power of developing complex interconnected systems capable of spanning multi-millimeter volumes in 3D with thousands of interconnected neuron spheroids and interneurons.​

Supplement Type

Standard, Minus Insulin, Minus Vitamin A

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