The table below lists examples of cells that have been successfully tested on the VitroGel 3D system. To get the best results from the VitroGel 3D system, the hydrogel conditions can be optimized depending on cell types and components of culture media.A test of cell growth in a gradient of hydrogel concentrations is recommended for each cell line.

Cell types/applicationsProduct usedCulture mediumDilution (v/v)
Hela cellsVitroGel 3D-RGDDMEM with 10%FBS1: 3
MCF-7 breast cancer cellsVitroGel 3D-RGDDMEM with 10%FBS1: 3
HCT-8 Human colon cancer cellsVitroGel 3D-RGDRPMI 1640 with 10% FBS1: 3
Human iPSCsVitroGel 3D-RGDmTeSR11: 3
Human embryonic kidney 293 cellsVitroGel 3D-RGDDMEM with 10%FBS1: 3
Beta TC3 cellsVitroGel 3D-RGDDMEM1: 3
Ins-1 cellsVitroGel 3D-RGDRPMI 16401: 3
BL5 human beta cellsVitroGel 3DDMEM with 10%FBS1: 3
Priess human lymphoblastoid cellsVitroGel 3DRPMI 1640 with 10% FBS1: 3
CD8+ T cellsVitroGel 3DRMPI 1640 with 10% FBS1: 3
Human isletsVitroGel 3D-RGDCMRL 1066 medium1: 3
DLD1 (colon)VitroGel 3D-RGDhigh glucose DMEM with 10%FBS1: 3
HepG2 (liver)VitroGel 3D-RGDhigh glucose DMEM with 10%FBS1: 3
HuH7VitroGel 3D-RGDDMEM1: 3
Prostate cancer lineVitroGel 3D-RGDRPMI 16401: 3
H460 cellsVitroGel 3D-RGDDMEM with 10%FBS1: 3
A549 cellsVitroGel 3D-RGDDMEM with 10%FBS1: 1
MCF-12AVitroGel 3D-RGDDMEM-F121: 3
SKB3 cellsVitroGel 3D-RGDDMEM with 10%FBS1: 3
MDA-MB-231VitroGel 3D-RGDMammosphere medium1: 3
T47DVitroGel 3D-RGDRPMI 1640 with 10% FBS1: 3
Au565VitroGel 3D-RGDRPMI 1640 with 10% FBS1: 3
Human Nthy-ori 3-1 cellsVitroGel 3DRPMI 1640 with 10% FBS1: 3
PDX models of cervix cancerVitroGel 3DAlpha MEM1: 2
4T1 murine breast cancer cellsVitroGel 3D-RGDRPMI 1640 with 10% FBS1: 2
Melanoma cell lines (M111, M101, and CG-1241)VitroGel 3D-RGDRPMI 16401: 3
BT 474VitroGel 3D-RGDDMEM+10% FBS1: 3
OVCAR-3VitroGel 3D-RGDRPMI-1640+10% FBS1: 1
U87-MGVitroGel 3D-RGDEMEM+10% FBS1: 2
UD 145VitroGel 3D-RGDEMEM+10% FBS1: 3
HCT116VitroGel 3D-RGDMcCoy's 5A+10% FBS1: 3

Case Studies

VitroGel 3D has been successfully used on different projects with many cell lines. Scientists are impressed by this incredibly easy to use hydrogel system and happy with the promising results. The case studies below show both 3D cell culture and 2D coating of VitroGel 3D system. In each case, all images were taken under the same scale.

Case 1: Beta TC3 cells (used VitroGel 3D-RGD for 3D culture)

Seeding number: 5×10cells/mL

Day 1

Day 5

Day 14

Case 2: Ins-1 cells(used VitroGel 3D-RGD for 3D culture)

Seeding number: 5×10cells/mL

Day 1

Day 5

Day 14

Case 3: Human Foreskin Fibroblast (hFS) derived Induced Pluripotent Stem Cells (iPSCs) (used VitroGel 3D-RGD for both 2D coating and 3D culture)

Seeding number: 2.5×10cells/mL

hFS iPSCs grown on 2D Matrigel coating (comparison)

Culture on 2D VitroGel 3D-RGD coating (Day 1)

Culture on 2D VitroGel 3D-RGD coating (Day 7)

3D culture using VitroGel 3D-RGD (Day 1)

3D culture using VitroGel 3D-RGD (Day 7)

Summary
  • Both 2D coating and 3D culture are suitable for hFS iPSC culture
  • hFS iPSCs in 2D coating conditions are more defined and rounded in morphology compared to 3D  culture conditions
  • Colonies are rounded as expected within 7 days in hydrogel

Case 4: Beta Lox 5 (BL5) cells (used both VitroGel 3D and VitroGel 3D-RGD for 2D coating and used VitroGel 3D for 3D culture)

Seeding number: 2.5×10cells/mL

2D cell culture on regular well-plate (control)

Culture on 2D VitroGel 3D coating for 7 days

Culture on 2D VitroGel 3D-RGD coating for 7 days

Normal human islets grew in suspension (Comparison)

3D culture using VitroGel 3D(Day 1)

3D culture using VitroGel 3D(Day 7)

Summary
  • BL5 cells grow very well in both 2D coating and 3D culture of VitroGel 3D system
  • Under 2D coating, BL5 cells form colonies and seem to be flat
  • Under 3D culture of VitroGel 3D, BL5 cells form islet-like structures very similar to normal human islets

Case 5: Human Embryonic kidney (HEK293) cells (used VitroGel 3D-RGD for 3D culture)

Seeding number: 2.5×10cells/mL

2D cell culture on regular well-plate (control)

3D culture using VitroGel 3D-RGD (Day 7)

Summary
  • HEK 293 cells grow in the form of clusters in 3D culture within VitroGel 3D-RGD

Case 6: Human lymphoblastoid Priess cells (used VitroGel 3D for both 2D coating and 3D culture)

Seeding number: 2.5×10cells/mL

Priess cells grown in suspension (control)

Culture on 2D VitroGel 3D coating for 7 days

3D culture using VitroGel 3D(Day 7)

Summary
  • Priess cells grown in both 2D coating and 3D culture conditions are similar to cells grown in suspension

Case 7:CD8+ T cells (use VitroGel 3D for 3D culture)

Seeding number: 2.5×10cells/mL

CD8+ T cells grew in suspension (control)

3D culture using VitroGel 3D(Day 7)

Summary
  • CD8+ T cells look vibrant in 3D culture conditions of VitroGel 3D