Case Studies, News
Complete Animal Origin-Free Platform for Organoid, NSC, iPSC
CASE STUDIES
TheWell Bioscience Inc.1 Deerpark Dr, Ste C, Monmouth Junction, NJ 08852, USA
Introduction
TheWell Bioscience’s VitroGel® hydrogels and RocketCell™ animal origin-free media, combined with PeptiGrowth’s synthetic, heat-stable peptide growth factors, form a fully synthetic culture platform that supports reliable growth and maintenance across three major stem cell applications — organoids, iPSCs, and neural stem cells — with the batch-to-batch consistency and reproducibility that animal-derived reagents can’t guarantee.
Organoid Culture
Mouse intestinal organoids cultured in VitroGel® ORGANOID with PeptiGrowth’s Synthetic EGF and Noggin-like Peptide showed strong growth, structural integrity, and cell viability over a 10-day culture period. Free of animal-derived raw materials and GMP-compatible, these synthetic growth factors perform as functional alternatives to conventional recombinant EGF and Noggin for intestinal organoid work.
iPSC Culture
PeptiGrowth’s FGFR1c Agonist paired seamlessly with RocketCell™ iPSC Growth Medium across both 2D monolayer and 3D (VitroGel® STEM) formats. iPSCs maintained key pluripotency markers (Oct4, Lin28) through 7 days in 2D, and in 3D culture the synthetic FGF2 alternative outperformed heat-stable FGF-2 — driving higher potency at lower concentrations for scalable, high-density iPSC expansion without sacrificing pluripotency.
NSC Culture
Neural stem cells cultured on VitroGel® NEURON with RocketCell™ NSC Media and PeptiGrowth’s Synthetic EGF and FGF2 alternative peptide matched — and after a brief ~24-hour adaptation period, outperformed — traditional Geltrex-based culture. Cells showed faster proliferation, more uniform morphology, reduced hydrogel usage, and consistent expression of the NSC marker Nestin, all in a fully animal origin-free system.
Key Insights:
- Discover a complete animal-origin-free workflow for organoid, iPSC, and NSC culture—from defined hydrogels and media to growth factors.
- See real application data demonstrating cell growth, viability, morphology, proliferation, and maintenance across 2D and 3D culture systems.
- Learn how to transition from animal-derived ECMs to synthetic, defined systems while maintaining robust cell culture performance
Download Case Study
Fill out the form below to access the complete case study including full methods, materials, and data for organoid, iPSC, and NSC culture.
Products Used
Matrigel® is a trademark of Corning Incorporated.
