In Vivio Study

VitroGel hydrogel system has a unique shear-thinning and rapid recovery rheological property. Under the mechanical shearing force such as injection through a syringe, the hydrogel performs a gel-sol transition and becomes free-flowing status. However, once the shearing force ceased, the mechanical strength of the hydrogel can rapidly recover with a sol-gel transition and become a hydrogel status again. With this injectable property, VitroGel can be used for in vivo cells/drug delivery for cell therapy or controlled release.

The hydrogel properties and preparing protocols can be manipulated for different physical/chemical characteristic and gel formation times to fulfill the requirements for different applications.

Mechanical Strength
In VitroGel, the hydrogel strength can adjust by a simple dilution process. The typical gel strength range from 10 to 4,000 pa and the customized higher concentration hydrogel can get over 20,000 Pa. 

Degradation
The MMP modified VitroGel can be used for this study. The hydrogel can mix and match with other version of VitroGel to study the process of MMP degradation and cell-matrix interaction for cell mobility.

Functional Ligands
With VitroGel, scientists can select hydrogel with different functional adhesive ligands, such as RGD, collagen, Laminin, and combine/vary the concentration to form a heterogeneous customized microenvironment.

Serum/Growth Factors/Cytokine/Chemokines
VitroGel is the xeno-free system. Bio-functional compounds can add to cell suspension before mixing with hydrogel to create an enriched hydrogel matrix or add after the hydrogel formation to feed cells or control release

Injectable Carrier for Cell Therapy
VitroGel system is biocompatible and safe for in vivo application. Using VitroGel as an injectable carrier, scientists can achieve better cell retention and higher cell viability for cell therapy

 

Getting to the root of the problem:  Injectable VitroGel® enhance odontogenic differentiation in vivo 

The study shows that the growth of apical papilla cells in VitroGel was able to promote hard tissue regeneration in vivo, a necessary phenotype for proper root development. Show in the image that the hydrogel encapsulated samples treated with both BMP-2 and SDF-1 produced the most osteoid dentin and secreted the most collagen matrix of all the samples. And semi-quantification of the vessels via CD-31 endothelial cell staining showed twice as many vessels as other samples. Xiao was also able to show that in vitro exposure of hydrogel-encapsulated cells to BMP-2 and SDF-1a, two factors individually shown to efficiently promote odontoblast differentiation, caused cells to express the early odontoblast marker DMP-1 and later marker of mineralized bone, OCN.

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VitroGel enhances stem cell transplantation to treat intervertebral disc degeneration:

Scientists use the tunable, injectable VitroGel RGD in combine with the MSCs derived from the nucleus pulpous (npMSCs) to address intervertebral disc degeneration in a rat model. VitroGel provides a promising and viable way to help promote the proliferation, differentiation, and survival of npMSCs in vitro and in vivo. This study has laid the groundwork for ongoing studies using injectable VitroGel in regenerative studies, both in intervertebral disc degeneration and other studies that rely heavily on proper ECM-cell interaction.

Read the Research Highlight for more information