RocketCell™ 3D NSC Complete Growth Kit, AOF

All-in-one, ready-to-use kit for 3D neural stem cell culture

Ready-to-use, All-in-one Kit
100% synthetic kit with optimal combination of matrix and medium for 2D and 3D NSC culture and maintenance.

Supports 3D NSC Network Formation
Promotes 3D neurite and axonal outgrowth for 3D networking formation, ideal for advanced studies.

Long-Term Culture and Maintenance
Culture NSCs for over 4 weeks without compromising their multipotency. 

Simple 3D Workflow at Room Temperature
Room temperature operation with simple mixing or coating steps; supports lab automation. 

The RocketCell™ 3D NSC Complete Growth Kit, AOF is an all-in-one, ready-to-use kit designed to support the expansion and maintenance of neural stem cells (NSCs) in both 3D and 2D formats. This kit offers a fully defined, optimized combination of a synthetic, chemically defined hydrogel and a animal origin-free complete medium, eliminating variability and ensuring consistent, reproducible results.

The RocketCell™ 3D NSC Complete Growth Kit, AOF provides a robust, scalable platform for long-term culture and large-scale expansion of undifferentiated NSCs while maintaining their multipotency and functional integrity. Designed for convenience and performance, this ready-to-use system operates at room temperature, integrates seamlessly with high-resolution imaging and high-throughput screening workflows for advanced neuroscience and regenerative medicine research.

The all-in-one kit includes:

3D Cell Culture:
RocketCell™ 3D NSC Complete Growth Kit, AOF (RC01-CGK2)

• 1 x 10 mL VitroGel® NEURON, the first-of-its-kind synthetic biofunctional hydrogel for NSC and neuronal cultures​
• 1 x 10 mL, RocketCell™ 3D NSC Suspension Medium​, AOF
• 1 x 500 mL, RocketCell™ NSC Basal Medium​, AOF
• 1 x 10 mL, RocketCell™ NSC Supplement (50X)​, AOF
• 2 x 50 µL, RocketCell™ Cell Viability Enhancer (1000X)
• 1 x 10 µg CytoGrow™ Recombinant EGF (Human), CHO, Tag Free
• 1 x 10 µg, CytoGrow™ Recombinant bFGF/FGF-2 (Human), E. coli, Tag Free

Also available:

• RocketCell™ NSC Growth Medium, AOF↗ 
  Cell culture medium for NSC culture

Specifications

Use	3D proliferation and maintenance of neural stem cells
Formulation	Synthetic, chemically defined, biofunctional hydrogel and animal origin-free complete growth media
Operation	Ready-to-use at room temperature
No. of Use	10 mL kit 6-well, 24-well, 96-well: 2 plates
Cell Harvesting	VitroGel® Organoid Recovery Solution​ 5-15 min cell recovery
Shipping and Storage	Hydrogel: Ships at ambient temperature. Store at 2-8°C. Basal Medium: Ships with ice pack. Store at 2-8°C. Supplement Dry Ice shipping. Store at -20°C Growth Factors: Ships with ice pack/dry ice. Store at -20- -80°C.

Challenges We Solve

Common Challenges in
NSC Culture

Lack of a complete animal origin-free platform for 3D NSC Culture

Poor cell matrix and cell-cell interaction for 3D neurite axonal outgrowth

Lack of an optimal combination of synthetic matrix and animal origin-free complete medium for NSC culture

Difficult to perform long-term NSC Culture

How RocketCell™ NSC Complete Growth Kit, AOF Solves The Issues​

Supports 3D NSC Culture
Enables both single cells and 3D spheroids to grow within VitroGel® NEURON hydrogel.

Enhanced 3D Cell Connectivity and Network Formation
Robust network formation and axonal projections mimicking the in vivo environment.

Complete all-in-one package
Optimal combination of synthetic, chemically defined hydrogel matrix and animal origin-free culture medium for robust and reliable results every time.

Long-Term Culture and Negligible Spontaneous Differentiation
Over 4 weeks in culture without compromising multipotency.

3D NEURON CULTURE 

Figure 1. 3D Encapsulation Culture of NSC cells as a Single Cell Suspension in VitroGel® NEURON​

VitroGel® NEURON Hydrogel and RocketCell™ NSC Media, AOF support 3D NSC cultures and neurite outgrowth. (A) 3D NSC cultures were established by preparing a single-cell NSC suspension with RocketCell™ NSC NSC Growth Medium, AOF and mixing with VitroGel® NEURON for 6 days. (B) Immunofluorescence staining was performed to evaluate the presence of beta-III-tubulin, a neuron maturation marker. The images were obtained with the Keyence BZ-X microscope at 10 and 20X magnifications.

Figure 2. RocketCell™ NSC Complete Growth Kit, AOF supports the expansion of NSC spheroids for differentiation with the RocketCell™ NSC-Neuron Differentiation Kit​.

iPSCs were 3D cultured in RocketCell™ 3D IPSC Complete Growth Kit, AOF and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Complete Growth Kit, AOF. The cells were then differentiated into neurons with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The samples were fixed and stained to evaluate the expression of (A) Microtubule-associated protein 2 (MAP2; green), (B) Neurofilament-H (red), and (C) Synaptophysin (purple). The nuclei were stained with Hoescht dye.

Figure 3. VitroGel® NEURON hydrogel supports the 3D development of dopaminergic neurons.​

iPSCs were 3D cultured in RocketCell™ 3D IPSC Complete Growth Kit, AOF for 6 passages and were differentiated into Floor Plate Neuro-Epithelial Spheroids. The spheroids were recovered from the gel using VitroGel® Organoid Recovery Solution and 3D cultured with the RocketCell™ NSC Complete Growth Kit, AOF (A, Day 0). To induce NSC differentiation into dopaminergic neurons, the media were replaced with the RocketCell™ NSC-Neuron Differentiation Growth Medium. The cells were exposed to the medium for 16 days (A, Day 16). The samples were fixed and stained with beta-III-tubulin and DAPI (B). It is apparent that most spheroids are neurons, and axonal connections are clearly visible at higher magnification. ​

Resources

Poster
Development of In Vitro Developing a Biofunctional Xeno-free Hydrogel Platform for Long-term 3D Neuronal Differentiation and Maintenance

Poster
The Growth and Neuronal Differentiation of Human Induced Pluripotent Stem Cells in Xeno-Free Functional Synthetic Hydrogel​

Enhance your neuron cell culture with these products:

NEW

Hydrogels - Ready-To-Use

VitroGel® NEURON

Ready-to-use, Synthetic, chemically defined hydrogel for neural stem cell and neuron cultures.

New

Cell Culture Media (Animal Origin-Free)

RocketCell™ NSC Growth Medium, AOF

Animal origin-free complete medium for 3D and 2D neural stem cell culture

New

Culture Media and Supplements (Animal Origin-Free)

RocketCell™ 3D iPSC Complete Growth Kit, AOF

An all-in-one, animal origin-free kit with optimized matrix, medium, and reagents for 3D iPSC expansion.

CytoGrow™ - Recombinant Proteins

EGF (Human), Recombinant Protein, CHO, Tag Free

Premium CytoGrow™ growth factor suitable for cell culture supplementation, wound healing research, epithelial biology, and cancer signaling studies.

CytoGrow™ - Recombinant Proteins

bFGF/FGF-2 (Human), Recombinant Protein, E. coli, Tag Free

Premium CytoGrow™ growth factor suitable for stem cell culture, angiogenesis research, wound healing studies, and regenerative medicine.

New

3D Culture Imaging Plates

VitroPrime™ 3D Culture and Imaging Plate

A premium cover-glass bottom plate for zero sample disruption 3D cell culture workflow: from cell seeding to high-resolution imaging.

3D Culture Vessels

VitroPrime™ Spread-Attach Plate

Unique surface treated for superior hydrogel spreading, adherence, and uniform surface.

Cell Harvesting Solution

VitroGel® Organoid Recovery Solution

Non-enzymatic cell harvesting solution to recover cells/organoids from hydrogel or an animal-based ECM within 15 minutes.

Downstream Cell Analysis

Cyto3D® Live-Dead Assay Kit

Fast (15 min), versatile, live/dead cell viability analysis for 3D and 2D cell culture.  IN STOCK