Poster Presentations

Generation of Apical-out Intestinal Organoids Using Xeno-free VitroGel® ORGANOID

Posted on by Kyra Baricaua
Poster for a talk on apical-out intestinal organoids using VitroGel ORGANOID, July 25, 2026, by Kalhara Menikdiwela, Ph.D., TheWell Bioscience.

Poster No. P02-06-26 at NUTRITION 2026

  • Poster Board Number: C-46
  • Poster Session: Applications of Novel in vitro Models in Nutrition Research
  • Presentation Date: July 25, 2026
  • Presentation Time: 11:30 A.M. -12:30 P.M.
  • Presenter: Kalhara Menikdiwela, Ph.D

Background:

  • Organoids are 3D self-organizing tissue models that closely mimic the architecture and function of native organs.​
  • Epithelial polarity is a fundamental feature of intestinal organoids, governing nutrient absorption, drug transport, and barrier function.​
  • Conventional Matrigel-grown intestinal organoids exhibit an apical-in orientation, restricting direct access to the absorptive epithelial surface.​
  • Apical-out intestinal organoids expose the brush border to the external environment, enabling direct interaction with nutrients, drugs, microbes, and
    other luminal factors.​
  • Apical-out polarity improves the physiological relevance of in vitro models for studying intestinal biology, nutrient uptake, and oral drug absorption.​
  • Most current organoid culture systems rely on animal-derived extracellular matrices, which introduce batch-to-batch variability and limit translational
    applications.​
  • Synthetic, xeno-free hydrogels provide a chemically defined and reproducible microenvironment for organoid culture, improving experimental consistency
    and scalability.​
  • Developing robust apical-out organoid models in synthetic hydrogels can enhance preclinical research in nutrition, drug discovery, disease modeling, and host–microbiome interactions.

Please complete the form below to request the poster.

ISSCR 2026 Poster - Xeno-Free iPSC Culture with VitroGel® & RocketCell™
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Products Used:

VitroGel® ORGANOID-5

RocketCell™ Organoid Essential-Core Xeno-Free Medium

CytoGrow™ Growth Factors

VitroPrime™ 3D Culture and Imaging Plate

Resources:

WEBINAR
Culture Apical-out Organoids with Xeno-free VitroGel® Hydrogel System

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